Secondary Metabolites of Microbials as Potential Pesticides

Maharana, Chandan; Padala, Vinod Kumar; Hubballi, Aravinda; Raj, M. Nikhil; Paschapur, Amit Umesh; Bhat, Chaitra; Singh, A. K.; Subbanna, A. R. N. S.

doi:10.1007/978-981-16-7695-6_5

Cited by 6 publications

(1 citation statement)

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“…The outer cuticle of the insects is usually composed of chitin, embedded with a protein matrix. The extracellular enzymatic compounds produced by EPF can degrade these chitinous compounds and obtain nutrients from the insect’s hemolymph 24 , 25 . The fungal chitinases usually belong to class 3 chitinases and are considered an important part of the family 18 of glycosyl hydrolases 26 .…”

Section: Introductionmentioning

confidence: 99%

Chitinase of Trichoderma longibrachiatum for control of Aphis gossypii in cotton plants

Anwar,

Amin,

Khan

et al. 2023

Sci Rep

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Chitinase-producing fungi have now engrossed attention as one of the potential agents for the control of insect pests. Entomopathogenic fungi are used in different regions of the world to control economically important insects. However, the role of fungal chitinases are not well studied in their infection mechanism to insects. In this study, Chitinase of entomopathogenic fungi Trichoderma longibrachiatum was evaluated to control Aphis gossypii. For this purpose, fungal chitinase (Chit1) gene from the genomic DNA of T. longibrachiatum were isolated, amplified and characterised. Genomic analysis of the amplified Chit1 showed that this gene has homology to family 18 of glycosyl hydrolyses. Further, Chit1 was expressed in the cotton plant for transient expression through the Geminivirus-mediated gene silencing vector derived from Cotton Leaf Crumple Virus (CLCrV). Transformed cotton plants showed greater chitinase activity than control, and they were resistant against nymphs and adults of A. gossypii. About 38.75% and 21.67% mortality of both nymphs and adults, respectively, were observed by using Chit1 of T. longibrachiatum. It is concluded that T. longibrachiatum showed promising results in controlling aphids by producing fungal chitinase in cotton plants and could be used as an effective method in the future.

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Section: Introductionmentioning

confidence: 99%