Secondary metabolites production of epigallocatechin gallate through in vitro culture of Camellia sinensis l with cinnamic acid precursors

Sutini,; Widiwurjani,; Augustien, Nora; Purwanto, Djoko; Muslihatin, Wirdhatul

doi:10.1063/5.0002565

Cited by 1 publication

(1 citation statement)

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“…This result is also supported by another study revealing that Bacillus sp. inoculation can produce indole-3acetic-acid (auxin) that induce plant growth and improve the synthesis of amino acids like phenylalanine and tyrosine, which enter shikimic acid pathway, which has the responsibility to product some phenolic compounds such as gallic acid, ferulic acid and p-coumaric acid (Cisternas-Jamet et al, 2020;Sutini et al, 2020). Gowtham et al (2018) also showed that Bacillus sp.…”

Section: Total Phenol Contentsmentioning

confidence: 99%

Fruit Morphology, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Salacca zalacca (Gaertner) Voss by Applications of Goat Manures and Bacillus velezensis B-27

Ilmiah

Sulistyaningsih

Joko

2021

Caraka Tani J. Sustain. Agric.

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Snake fruit (Salacca zalacca (Gaertner) Voss) is one of indigenous fruits from Southeast Asia that has been consumed for its antioxidant contents. Improving the fruit quality might increase its benefits for human health. This study aims to analyze fruit morphology, antioxidant activity, total phenolic and flavonoid contents of the fruit applied with goat manures and Bacillus velezensis B-27. The research used two-factor Randomized Complete Block Design (RCBD) with three blocks as replications. The first factor is the level of goat manure application i.e. 0 kg plant-1, 5 kg plant-1 and 10 kg plant-1, while the second factor is the bacteria application i.e. applied with Bacillus velezensis B-27 and without Bacillus velezensis B-27. The research was conducted in June until December 2019 at Turi, Sleman, Yogyakarta, Indonesia. Fruit length, diameter and shape were measured as morphology parameters. Antioxidant activities were measured using 2,2-Diphenyl-1-picrylhydrazyl, whereas flavonoid and phenolic contents were determined by the aluminium-chloride colorimetric and Folin-Ciocalteu methods, respectively. Means of each parameter were analyzed using ANOVA, continued with the Tukey’s HSD test at a 5% significance level. The result showed that the application of 10 kg goat manure per plant with bacteria increased the fruit length (76.78 mm) and the diameter (62.72 mm). Addition of 10 kg goat manure per-plant combined with Bacillus velezensis B-27 gave the highest antioxidant (IC50 of 37.83 µg.mL-1), flavonoid (5.35 mgGAE.100 g-1) and total phenolic contents (44 mgQE.100 g-1).

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Section: Total Phenol Contentsmentioning

confidence: 99%