Secondary osteoporosis in collagen-induced arthritis rats

Abstract

Numerous studies have demonstrated that rheumatoid arthritis (RA) is often associated with bone loss; however, few experiments have focused on cancellous and cortical bone changes in rats during the process of arthritis. We have investigated bone changes in rats with collagen-induced arthritis (CIA) and have explored the characteristics of how RA induces osteoporosis by means of bone histomorphometry, bone biomechanics studies, bone mineral density studies, micro computer tomography, enzyme-linked immunosorbant assay, immunohistochemistry, and Western blot analysis. Bone mineral density of the femur and lumbar vertebrae and biomechanical properties of the femur were decreased in CIA rats. Trabecular bone volume of the tibia and lumbar vertebrae was decreased whereas bone resorption was increased in CIA rats. Bone formation of the tibial shaft in periosteal surfaces was decreased in CIA rats. Furthermore, the trabecular bone loss in CIA rats was severer at 16 weeks than at 8 weeks, as was cortical bone loss. The serum level of tumor necrosis factor α in CIA rats was increased, and the expression of dickkopf 1 and that of receptor activator of nuclear factor κB (RANKL) ligand (RANKL) in the ankle joints were also increased, but the expression of osteoprotegerin (OPG) was decreased. We conclude that CIA rats developed systemic osteoporosis, and that osteoporosis became more serious with CIA development. The mechanism may be related to the increase of bone resorption in cancellous bone cause by upregulation of the expression of DKK-1 and regulation of the RANKL/RANK/OPG signaling pathway, and the decrease of bone formation in cortical bone caused by an increase in the expression of DKK-1.

Myeloid thrombomodulin lectin-like domain inhibits osteoclastogenesis and inflammatory bone loss

“…In contrast, high-turnover osteoporosis indicates a condition where increased bone resorption results from increased osteoclast activity. Low-turnover osteoporosis is generally induced by corticosteroids, whereas high-turnover osteoporosis is associated with estrogen deficiency or rheumatoid arthritis and other chronic inflammatory disorders2728.…”

The Effect of Ozone on Bone Strenght in Animal Model of Rheumatoid Arthritis

“…Bu sonuç ozonun organizma için sitokinler üzerinden zararlı bir etki oluşturmadığını da göstermektedir. RA sinovyal inflamasyonla karakterize, kemik ve kıkırdakta destrüksiyona yol açan otoimmün bir hastalıktır (30 (14) ise ozon tedavisi sonrasında serum TNF-α düzeylerinde değişiklik olmadığını, ancak intraartiküler TNF-α düzeylerinin azaldığını saptamışlar. Sonucun bizim çalışmadan farklı olması bu çalışmada ozonun intraartiküler olarak uygulanmasından kaynaklanıyor olabilir.…”

“…Bunun yanı sıra ozonun nöro-endokrin aksı stimüle ederek tedavi süresince hastanın kendisini daha iyi hissetmesini sağladığı da gösterilmiştir (40). RA'da periartiküler ve sistemik osteoporoz normal popülasyondan daha fazladır (30). FCAA'nın RA'ya bağlı osteoporozu belirlemede uygun bir model olduğu önceki çalışmalarla gösterilmiştir (30,(40)(41)(42) (30,46).…”

“…RA'da periartiküler ve sistemik osteoporoz normal popülasyondan daha fazladır (30). FCAA'nın RA'ya bağlı osteoporozu belirlemede uygun bir model olduğu önceki çalışmalarla gösterilmiştir (30,(40)(41)(42) (30,46). Gerek bu, gerekse önceki çalışmalarda ozon uygulamasının özellikle serum TNF-α düzeylerini olumlu etkilediği gösterilmiştir (13,14,38,39).…”

Micro-CT imaging analysis for the effects of ibandronate and eldecalcitol on secondary osteoporosis and arthritis in adjuvant-induced arthritis rats

“…Clinical signs of arthritis in each hind paw were assessed once weekly by investigators blind to the treatment group, as previously described (25,42). Hind footpad width was also measured once weekly with calipers (Dial Thickness Gauge; Ozaki Mfg.…”