Secondary structure of mouse 28S rRNA and general model for the folding of the large rRNA in eukaryotes

Abstract

We present a secondary structure model for the entire sequence of mouse 28S rRNA (1) which is based on an extensive comparative analysis of the available eukaryotic sequences, i.e. yeast (2, 3), Physarum polycephalum (4), Xenopus laevis (5) and rat (6). It has been derived with close reference to the models previously proposed for yeast 26S rRNA (2) and for prokaryotic 23S rRNA (7-9). Examination of the recently published eukaryotic sequences confirms that all pro- and eukaryotic large rRNAs share a largely conserved secondary structure core, as already apparent from the previous analysis of yeast 26S rRNA (2). These new comparative data confirm most features of the yeast model (2). They also provide the basis for a few modifications and for new proposals which extend the boundaries of the common structural core (now representing about 85% of E. coli 23S rRNA length) and bring new insights for tracing the structural evolution, in higher eukaryotes, of the domains which have no prokaryotic equivalent and are inserted at specific locations within the common structural core of the large subunit rRNA.

Variability of D2/D3 segment sequences of several populations and pathotypes of potato cyst nematodes (Globodera rostochiensis, Globodera pallida)

“…The secondary structure of D2/D3 segments of both species matches the general eukaryote model (Michot et al 1984).…”

Structure and function of ribosomal RNA

“…Glotz et al, 1981;Branlant et al, 1981) as well as for eukaryotic 26S RNA (Veldman et al, 1981) and 28S RNA (e.g. Michot et al, 1984;Hadjiolov et al, 1984). The equivalence of eukaryotic 5.8S RNA to the 5'-terminal region of prokaryotic 23S RNA has already been mentioned in the previous section, and the interaction between 5.8S RNA and the 5'-region of the 26S or 28S RNA is now well-documented (Vaughn et al, 1984;Walker et al, 1983;see Brimacombe et al, 1983, for review).…”

Morphology and rDNA phylogeny of a Mediterranean <em>Coolia monotis</em> (Dinophyceae) strain from Greece

“…D1-D6 are phylogenetically useful variable domains ("D" regions; Hassouna et al, 1984;Michot et al, 1984) within the LSU sequence of dinoflagellates Edvardsen et al, 2003;Murray et al, 2004). The SSU sequences evolve much slower and therefore provide information for comparing groups at a higher taxonomic level than the LSU permits; however, they are also used for distinguishing between species (Saunders et al, 1997;Grzebyk et al, 1998;Saldarriaga et al, 2001).…”