1988
DOI: 10.1007/bf02009166
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Secretin-regulated chloride channel on the apical plasma membrane of pancreatic duct cells

Abstract: Using the patch clamp technique we have identified a small conductance ion channel that typically occurs in clusters on the apical plasma membrane of pancreatic duct cells. The cell-attached current/voltage (I/V) relationship was linear and gave a single channel conductance of about 4 pS. Since the reversal potential was close to the resting membrane potential of the cell, and unaffected by changing from Na+-rich to K+-rich pipette solutions, the channel selects for anions over cations in cell-attached patches… Show more

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Cited by 189 publications
(140 citation statements)
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“…A low-conductance Cl channel has been reported in the shark rectal gland which might have similar conductance under comparable experimental conditions ( [25]; 11 pS, 2O"C, 280 Cl); however, its gating behaviour is different from the low-conductance channel in Ts4 CellS and it iS frequently observed in excised patches. Another Cl channel which strongly resembles the one described here occurs in the apical membrane of rat (4 pS) and human (7 pS) pancreatic duct, where it is believed to mediate Cl recycling at the apical membrane during secretin-stimulated HC03 secretion [21,22]. A similar channel has also been demonstrated recently in cultured thyroid gland epithelium (5.5 pS), where it may be involved in thyrotrophin-modulated fluid transport [23].…”
Section: Discussionsupporting
confidence: 68%
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“…A low-conductance Cl channel has been reported in the shark rectal gland which might have similar conductance under comparable experimental conditions ( [25]; 11 pS, 2O"C, 280 Cl); however, its gating behaviour is different from the low-conductance channel in Ts4 CellS and it iS frequently observed in excised patches. Another Cl channel which strongly resembles the one described here occurs in the apical membrane of rat (4 pS) and human (7 pS) pancreatic duct, where it is believed to mediate Cl recycling at the apical membrane during secretin-stimulated HC03 secretion [21,22]. A similar channel has also been demonstrated recently in cultured thyroid gland epithelium (5.5 pS), where it may be involved in thyrotrophin-modulated fluid transport [23].…”
Section: Discussionsupporting
confidence: 68%
“…The low-conductance channel in T84 CdS iS SiIIIikir t0 those described previously in pancreatic duct [21,22] and thyroid gland [23]; except for its somewhat higher conductance (8.7 vs 5-7 pS). To determine if temperature could account for this difference we measured single-channel currents as the bath was allowed to cool.…”
Section: S Temperature Dependence Of Low-conductance CL Channelsupporting
confidence: 61%
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“…CFTR also regulates secretion of mucins and serous proteins in epithelial cells (5,6). Under normal physiological conditions, opening of the CFTR channel is triggered by secretagogues that elevate intracellular cAMP (4,7), resulting in protein kinase A-mediated phosphorylation at multiple sites on the R domain (8). In CF, mutations in the gene produce proteins that are not correctly processed * This work was supported by doctoral fellowships from the Association Française de Lutte contre la Mucoviscidose (AFLM) (to C. M.-M. and R. D.), by institutional grants from INSERM and CNRS, and by specific grants from AFLM and the CF Trust, United Kingdom.…”
Section: Chloride Channels Play An Important Role In the Physiology Amentioning
confidence: 99%
“…X.-S. Yang Medicine,Japan Secretion of HC0 3 -across the luminal membrane of pancreatic duct epithelium is usually attributed to Cl--HC0 3 -exchange with Cl-recycling via CFTR Clchannels (Gray et al 1988). In the guinea-pig, the secreted fluid may contain HC0 3 -at concentrations as high as 150 mM, and it is questionable whether the luminal Clconcentration would be suffici ent to drive Cl--HC0 3 -exchange.…”
mentioning
confidence: 99%