Secretion of angiogenic activity by placental tissues of cows at several stages of gestation

Growth and in-vitro metabolism of placental tissues of cows from Day 100 to Day 250 of gestation

“…In the present study, the in-vitro rate of oxygen consumption by bovine placental tissues was similar to that reported by us and others for ovine placental tissues (Bell et al, 1987a;Reynolds & Redmer, 1987). The large proportion of 02 taken up by the gravid uterus and utilized to support placental metabolism varies from «80% at mid-gestation to «40% during late gestation in cows and ewes (Battaglia & Meschia, 1981;Bell et al, 1986;Reynolds et al, 1986).…”

“…In the present study, in-vitro uptake of 02 by maternal placental tissues was comparable to that reported for placental tissues in vivo and was 2-3-fold greater than 02 uptake of fetal placental tissues. Similar observations have been reported for sheep placental tissues (Reynolds & Redmer, 1987). Therefore, maternal placental tissues probably account for a major portion of the high metabolic demand of the placenta.…”

Cell culture on thin tissue sections commonly prepared for histopathology

“…Bovine placental tissues have angiogenic activity (27). Also, the conditioned media of placental explants obtained from cows during days 100–200 of gestation support the migration of bovine aortic endothelial cells (28). This angiogenic activity may have relevance to the expression of heparanase in bovine placenta during gestation (29).…”

Imunolocalização do bFGF em corpo lúteo de búfalas em diferentes estágios do ciclo estral

“…SHING, 1992;KUWANO et al, 1994, LIN et al, 2002. Sabe-se que o rápido aumento da vascularização luteínica é estimulado por fatores angiogênicos produzidos no próprio corpo lúteo (REYNOLDS;REDMER, 1988), como bFGF (basic fibroblast growth factor (GRAZUL-BILSKA et al, 1992;GRAZUL-BILSKA et al, 1993;BERISHA et al, 2000;SCHAMS et al, 2001) e VEGF (vascular endothelial growth factor) (PLENDL, 2000).…”

“…Embora alguns estudos tenham caracterizado a expressão desses fatores de crescimento no corpo lúteo dos bovinos, tanto durante o ciclo estral quanto ao longo da gestação (REYNOLDS et al, 1988;BERISHA et al, 2000;HAYASHI et al, 2000), até o presente momento existe apenas um estudo realizado em nosso grupo a respeito da expressão do VEGF no corpo lúteo bubalino (MOURA, 2003). Assim sendo, o presente estudo hipotetiza que a expressão da proteína e do mRNA do bFGF em corpo lúteo de búfalas varia de acordo com os diferentes estágios do ciclo estral e que o corpo lúteo de búfalas submetidas à superovulação se comporta diferentemente em relação à expressão deste fator.…”