Secretion of Growth Hormone (GH)-Releasin Hormone by GH-Producing Pituitary Adenoma Assessed by Cell Immunoblot Analysis.

Kawamoto, Keiichi; Uozumi, Tohru; Arita, Kazunori; Takechi, Akihiko; Kawamoto, Hitoshi

doi:10.1507/endocrj.42.89

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…After the colour reaction, the membranes were rinsed with distilled water for 10 min and allowed to dry in air. These procedures for blocking and staining were essentially performed according to the method described by Arita et al [1,12] with some modifications. The specificity of the cell immunoblot for MMP-9 was confirmed by omitting the antiserum, under which conditions no cells were detected.…”

Section: Cell Lmmunoblot Assaymentioning

confidence: 99%

Matrix metalloproteinase-9 secretion by human pituitary adenomas detected by cell immunoblot analysis

Kawamoto

Mizoue

et al. 1996

Acta neurochir

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Twenty-two pituitary adenomas were examined on the secretion of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) using a cell immunoblot assay, and discussed regarding an association between cavernous sinus invasion and the secretion of these proteins. The cell immunoblot assay, a kind of immunoblot procedure, is able to detect proteins at the single cell level and to detect the incidence of tumour cells secreting the target proteins in the total tumour cell population. The incidence of tumour cells secreting MMP-9 was significantly higher in invasive adenomas than in noninvasive ones. On the other hand, TIMP-1 secretion was not detected in any adenomas in this study. This result suggested that MMP-9 secretion, and especially the number of MMP-9-secreting cells, may be associated with cavernous sinus invasion of pituitary adenomas.

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Section: Cell Lmmunoblot Assaymentioning

confidence: 99%

Matrix metalloproteinase-9 secretion by human pituitary adenomas detected by cell immunoblot analysis

Kawamoto

Mizoue

et al. 1996

Acta neurochir

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Secretory activity of gonadotropin and the responsiveness of gonadotrophs to gonadotropin-releasing hormone during the annual reproductive cycle of male bats,Rhinolophus ferrumequinum: Analysis by cell immunoblot assay

2000

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Secretion of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 by Meningiomas Detected by Cell Immunoblot Analysis

Mizoue

Kawamoto

Arita

et al. 1999

Acta Neurochirurgica

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To identify features of meningiomas that infiltrate dura mater, and to examine the role Matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) secretion in meningiomas, a cell immunoblot assay study was performed in 20 meningiomas. MMP-9 secretion was detected in 20 (100%) meningiomas and was independent of histological features. TIMP-1 secretion was detected in 8 (40%) meningiomas. For a cell immunoblot volume which included approximately 1350 cells, there were 50.4 +/- 49.4 dots which showed immunoreactivity to MMP-9 and 2.0 +/- 4.2 which showed immunoreactivity to TIMP-1. The number of dots that showed immunoreactivity to MMP-9 was not significantly different between tumours with atypical and benign histological features. TIMP-1 secretion was found in only 8 (40%) specimens and the percentage of TIMP-1 secreting cells was significantly lower than that of MMP-9 secretion. Thus, we propose that meningiomas classified histologically as both atypical and benign have the potential to infiltrate dura mater.

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Secretion of Growth Hormone (GH)-Releasin Hormone by GH-Producing Pituitary Adenoma Assessed by Cell Immunoblot Analysis.

Cited by 4 publications

References 17 publications

Matrix metalloproteinase-9 secretion by human pituitary adenomas detected by cell immunoblot analysis

Matrix metalloproteinase-9 secretion by human pituitary adenomas detected by cell immunoblot analysis

Secretory activity of gonadotropin and the responsiveness of gonadotrophs to gonadotropin-releasing hormone during the annual reproductive cycle of male bats,Rhinolophus ferrumequinum: Analysis by cell immunoblot assay

Secretion of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 by Meningiomas Detected by Cell Immunoblot Analysis

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