Secretion of prostaglandins and progesterone by cells from corpora lutea of mares

Watson, E.D.; Sertich, Patricia L.

doi:10.1530/jrf.0.0880223

Cited by 39 publications

(18 citation statements)

References 9 publications

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“…Therefore, in a recent study we examined whether P4 and its precursor pregnenolone may affect the synthesis of the luteotrophic PGE2 in the equine luteal tissue (Ferreira-Dias et al 2006b). Mare's early and mid-luteal phases CL were able to release PGE2 in vitro (Fig 2), as previously reported for equine dispersed luteal cells (Watson and Sertich 1990). There was a significant increase in the production of this eicosanoid from early-luteal phase to mid-luteal phase CL.…”

Section: Physiological Mechanisms Of Regulation Of CL Formationsupporting

confidence: 85%

Some aspects of regulation of luteal function and luteolysis in equine corpora lutea

Ferreira‐Dias¹,

Skarzynski²

2008

PHK

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SummaryLike in other mammalian species, in the mare, in every ovarian cycle, a complex process of luteinization leads to corpus luteum (CL) formation. The CL produces progesterone (P4), a required hormone for establishment and maintenance of early pregnancy. Luteal angiogenesis in the mare, necessary for the CL formation, is followed by vascular regression during tissue involution, coordinated with the development and regression of non-vascular tissue (steroidogenic cells included). These processes might be regulated by several factors, still not completely understood. This work reviews some of our studies on this field in the mare. In the equine CL, tumour necrosis factor (TNFα) and nitric oxide (NO) may stimulate the production of angiogenic factor(s) and prostaglandin (PG) E2, necessary for vascular and non-vascular development during the early luteal phase. Increased microvascularization is accompanied by the synthesis of P4, rise in P4 receptor number and an increase in the number of large luteal cells. This suggests the importance of large luteal cells in the auto/paracrine regulation of P4 synthesis in the equine CL. However, when angiogenesis is complete, long term exposure to progestagens appears to inhibit the proliferative/angiogenic activity. PGF2α may also induce luteal (including vascular) regression of the equine CL. Moreover, NO and P4 dependent process of inhibition of angiogenic factor(s) production might be preparing the CL for functional and structural regression. Oppositely to the early luteal phase, during late luteal phase NO and cytokines may induce several processes leading to the regression of equine CL. Besides, caspase-3, the effector enzyme of apoptosis, might also play an important role during luteal tissue involution. Nevertheless, further studies are needed to elucidate the mechanisms and the relationship between the dichotomy of loss of function and/or luteal involution in the mare.

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Section: Physiological Mechanisms Of Regulation Of CL Formationsupporting

confidence: 85%

Some aspects of regulation of luteal function and luteolysis in equine corpora lutea

Ferreira‐Dias¹,

Skarzynski²

2008

PHK

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“…Prostaglandins are known to play important role in the ovulatory process during which levels of PGE2 and PGF2a are highly increased just prior to ovulation (Espey 1980, Watson & Sertich 1990, Sirois & Doré 1997, Watson & Hinrichs 1998. PGDH is an important enzyme involved in PGE2 and PGF2a catabolism.…”

Section: Discussionmentioning

confidence: 99%

“…This process is accompanied with a selective induction of PGHS-2 in granulosa cells and a marked increase in intrafollicular levels of PGE2 and PGF2a (Espey 1980, Watson & Sertich 1990, Sirois & Doré 1997, Watson & Hinrichs 1998. Studies on the regulation of PGDH during ovulation are limited to one recent report from the preovulatory follicle of a primate (Duffy et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Cloning of equine prostaglandin dehydrogenase and its gonadotropin-dependent regulation in theca and mural granulosa cells of equine preovulatory follicles during the ovulatory process

Sayasith

Bouchard²,

Doré³

et al. 2007

Reproduction

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The mammalian ovulatory process is accompanied by a gonadotropin-dependent increase in follicular levels of prostaglandin E2 (PGE2) and PGF2a, which are metabolized by 15-hydroxy prostaglandin dehydrogenase (PGDH). Little is known about ovarian PGDH regulation in non-primate species. The objectives of this study were to characterize the structure of equine PGDH and its regulation in follicles during human chorionic gonadotropin (hCG)-induced ovulation. The full-length equine PGDH was obtained by RT-PCR, 5 0 -and 3 0 -rapid amplification of cDNA ends (RACE). Its open reading frame encodes a 266-amino acid protein that is 72-95% homologous to other species. Semi-quantitative RT-PCR/Southern blot were used to study PGDH regulation in follicles isolated 0-39 h post-hCG. Results showed that PGDH mRNA expression was low in follicles obtained at 0 h, increased at 12 and 24 h (P!0.05), and decreased at 36-h post-hCG. This induction of expression was biphasic, with elevated abundance of transcripts at 12 and 33 h post-hCG (P!0.05) in mural granulosa and theca cells. Immunohistochemistry and immunoblotting confirmed regulated expression of PGHD protein in both cell types of preovulatory follicles after hCG. High levels of PGDH mRNA were observed in corpus luteum and other non-ovarian tissues tested, except kidney, muscle, brain, and heart. Thus, this study is the first to report the gonadotropin-dependent regulation of PGDH during ovulation in a non-primate species. PGDH induction was biphasic in theca and mural granulosa cells differing from primates in which this induction was monophasic and limited to granulosa cells, suggesting species-specific differences in follicular control of PGDH expression during ovulation.Reproduction (

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“…During early pregnancy, days 13-17, endogenous uterine PGF2u secretion into the uterine vein is reduced (Gleeson et ai, 1974;Moeljono et ai, 1977), and luteal PGF2a receptor concentrations are considerably lower than in nonpregnant pigs (Gadsby et ai, 1990). However, the corpus luteum of cows (Shemesh & Hansel, 1975;Milvae & Hansel, 1983;Pate, 1988), ewes (Rexroad & Guthrie, 1979), women (Challis et al, 1976), mares (Watson & Sertich, 1990), monkeys (Johnson et al, 1988;Ottobre et al, 1989), rabbits (Schlegel et al, 1988;Schlegel & Daniels, 1989) and cyclic (Patek & Watson, 1976;Walker et al, 1977;Guthrie et al, 1978) or pregnant pigs (Watson & Patek, 1979; Guthrie & Rexroad, 1981) can synthesize prostaglandins.…”

Section: Introductionmentioning

confidence: 99%

Effect of prostaglandins on luteal function during early pregnancy in pigs

Więsak

Hunter²,

Foxcroft³

1992

Reproduction

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Summary. Luteal cells were obtained by digestion of luteal tissue of cyclic (day 12) and early pregnant (days 12, 20 and 30) pigs. Suspensions of the dispersed luteal cells (5 x 104 cells ml \m=-\1) were incubated for 2 h in minimum essential medium (MEM) alone (control) and MEM with different concentrations of prostaglandin FF2\ g=a\(PGF2\g=a\) and PGE2 (0\m=.\01,0\m=.\1,1, 10, 100 and 1000 ng ml \m=-\1) and luteinizing hormone (LH) 100 and 1000 ng ml\m=-\1, or with combinations of LH+ PGF2\g=a\and LH + PGE2. Net progesterone production was measured in the incubation media by direct radioimmunoassay.The overall response pattern of the luteal cells to exogenous hormones on day 12 of the oestrous cycle and pregnancy differed (P < 0\m=.\5) from treatment on day 20 and 30 of pregnancy. In general progesterone production was higher (P < 0\m=.\5) and the response to PGF2\g=a\ and PGE2 treatment was most obvious on day 12 of the oestrous cycle and pregnancy. Overall, PGF2\g=a\ stimulated progesterone production in a dosedependent manner (P < 0\m=.\05). The response to PGE2 was of a quadratic nature (P < 0\m=.\05) in which the lowest and the highest doses of PGE2 were associated with a greater production of progesterone than were the intermediate doses. Treatment of luteal cells with PGF2\g=a\ + LH or PGE2 + LH caused overall inhibition (P < 0\m=.\05) of progesterone production compared with treatment with each hormone alone. This interaction was not affected by the dose of LH used.These findings indicate that PGF2\g=a\ and PGE2 are involved in the autocrine control of corpus luteum function.

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Secretion of prostaglandins and progesterone by cells from corpora lutea of mares

Abstract: Summary. Corpora lutea (CL)

Cited by 39 publications

References 9 publications

Some aspects of regulation of luteal function and luteolysis in equine corpora lutea

Some aspects of regulation of luteal function and luteolysis in equine corpora lutea

Cloning of equine prostaglandin dehydrogenase and its gonadotropin-dependent regulation in theca and mural granulosa cells of equine preovulatory follicles during the ovulatory process

Effect of prostaglandins on luteal function during early pregnancy in pigs

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