Secretor status and humoral immune responses to <i>Neisseria lactamica</i> and <i>Neisseria meningitidis</i>

Zorgani, Abdulaziz; Stewart, Joanna; Blackwell, C. Caroline; Elton, R. A.; Weir, D. M.

doi:10.1017/s0950268800050433

Cited by 14 publications

(16 citation statements)

References 21 publications

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“…An ELISA to detect the anti-idiotypic IgG against anti-Lewis a was adapted from that used in previous studies [19]. Two-fold dilutions of the affinity purified monoclonal IgM antibody used to immunise the mice were prepared (1 in 10 to 1 in 80) in coating buffer (15 mM NaCO3, 35 mM NaH2CO 3 3 mM NAN3, pH 9.6).…”

Section: Detection Of Anti-idiotypic Antibodiesmentioning

confidence: 99%

Detection of microbial surface antigens that bind Lewisa antigen

Essery

Weir

James

et al. 1994

FEMS Immunol Med Microbiol

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There is evidence that the Lewis(a) blood group antigen is one of the receptors for a number of potentially pathogenic microorganisms. To determine how widely distributed the microbial adhesins are that bind this antigen, anti-idiotypic antibodies produced against monoclonal anti-Lewis(a) were used in coagglutination assays to screen a variety of species. The following were agglutinated: 7/7 strains of Staphylococcus aureus; 10/19 (53%) strains of Neisseria meningitidis; 8/13 (62%) strains of Haemophilus influenzae; 1/3 strains of Helicobacter pylori; 1/2 strains of Neisseria gonorrhoeae; 1/2 strains of Candida albicans. The application of the anti-idiotypic antibodies to studies of host cell receptors, isolation of adhesins and development of new epidemiological typing reagents is discussed.

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Section: Detection Of Anti-idiotypic Antibodiesmentioning

confidence: 99%

Detection of microbial surface antigens that bind Lewisa antigen

Essery

Weir

James

et al. 1994

FEMS Immunol Med Microbiol

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“…Individuals who are non-secretors of their ABO blood group antigens are over-represented among patients with disease due to Neisseria meningitidis [3]. In a previous study we demonstrated that non-secretors had lower levels of total salivary IgM and salivary IgM specific for Neisseria lactamica and five strains of meningococci [4]. In this study, a flow cytometry assay was adapted from that described by Saadi et al [5] to assess the effect of salivary components on attachment of meningococci to epithelial cells.…”

Section: Introductionmentioning

confidence: 77%

“…The elute was dialysed against three changes of PBS. The presence and purity of IgA, IgG and IgM antibodies was tested using an ELISA system [4].…”

Section: Antibody Purification With Sepharose Beadsmentioning

confidence: 99%

Inhibitory effect of saliva from secretors and non-secretors on binding of meningococci to epithelial cells

Zorgani¹,

Stewart²,

Blackwell³

et al. 1994

FEMS Immunology &Amp; Medical Microbiology

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Carriage of Neisseria meningitidis B:4:P1.15 was higher among non-secretors during a school outbreak of meningitis; non-secretors had lower levels of anti-meningococcal salivary IgM. Flow cytometry was used to assess effects of secretor and non-secretor saliva on binding of B:4:P1.15 to buccal epithelial cells: (1) to assess inhibition by IgA and IgM; and (2) to assess contributions of salivary antibodies to inhibitory activities. Greater inhibition was obtained with secretor saliva: pooled (P = 0.049); fresh (P = 0.0001). Purified IgA (P = 0.02) and IgM (P = 0.03) were equally inhibitory. After absorption of anti-meningococcal antibodies, there was still significant inhibitory activity in the pools: secretors (P = 0.018); non-secretors (P = 0.005). These results indicate that both secretory immunoglobulins and other factors contribute to protection against colonisation by meningococci and might explain the increased carriage of B:4:1.15 in this population.

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“…The micro-method described previously was used (Zorgani et al, 1992). The complement source was prepared from serum of a donor with blood-group O.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Moraxella catarrhalis isolates from children and adults for growth on modified New York City medium and potential virulence factors

Ahmer

Braun

Amyes

et al. 2003

Journal of Medical Microbiology

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Initial studies found that Moraxella catarrhalis isolates from adults that grew on modified New York City medium (MNYC þ ) that contained antibiotics selective for pathogenic neisseriae differed from strains that did not grow on this medium (MNYC À ) in their potential virulence properties. It was predicted that higher usage of antibiotics to treat respiratory illness in children might result in higher proportions of MNYC þ isolates if antibiotics were an important selective pressure for this phenotype. Two of 100 adult isolates (2 %) were MNYC þ , compared to 88 of 88 isolates (100 %) from children (P ¼ 0·000). MNYC þ strains were serum-resistant and bound in higher numbers to HEp-2 cells that were infected with respiratory syncytial virus (RSV). Endotoxin from an MNYCþ isolate induced significantly higher pro-inflammatory response levels than endotoxin from an MNYC À strain. MNYC À adult isolates expressed haemagglutinins and bound in lower numbers to RSV-infected cells, but serum resistance was variable. All isolates from children were MNYC þ , serum-resistant and bound in greater numbers to RSV-infected cells. These results indicate that both RSV infection and antibiotic usage select for the MNYC þ phenotype.

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Secretor status and humoral immune responses to Neisseria lactamica and Neisseria meningitidis

Cited by 14 publications

References 21 publications

Detection of microbial surface antigens that bind Lewisa antigen

Detection of microbial surface antigens that bind Lewisa antigen

Inhibitory effect of saliva from secretors and non-secretors on binding of meningococci to epithelial cells

Comparison of Moraxella catarrhalis isolates from children and adults for growth on modified New York City medium and potential virulence factors

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