Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface

Choi, Byeong-Dae; Lee, Seung-yeon; Jeong, Soon‐Jeong; Lim, Do-Seon; Cha, Hee‐Jae; Chung, Won-Gyun; Jeong, Moon‐Jin

doi:10.3892/mmr.2016.5381

Cited by 16 publications

(14 citation statements)

References 27 publications

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“…Our results corroborate findings of osteogenesis-angiogenesis coupling in vivo [ 50 ] that may have been mediated by the elevated expression of CTGF [ 51 , 52 ] and HIF1A [ 53 ] in HWJSC spheroids during osteogenic differentiation. We examined the top 10 upregulated genes in day 7 versus day 1 HWJSC spheroids and observed that 5 out of the 10 are directly ( ZBTB16 [ 54 – 56 ]) or indirectly related to osteogenesis or mineralization ( SAA1 and SAA2 [ 57 ], SLPI [ 58 ], IL1RL1 [ 59 ]), while upregulated HMCN2 may be attributed to its role in calcium regulation and cell binding via gene ontology. We observed an increase in STAT3 and IL1RL1 expression in day 7 versus day 1 HWJSC spheroids consistent with elevated IL1RL1 in a STAT3 overexpression model exhibiting osteoblast differentiation[ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Engineering human cell spheroids to model embryonic tissue fusion in vitro

et al. 2017

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Epithelial-mesenchymal interactions drive embryonic fusion events during development, and perturbations of these interactions can result in birth defects. Cleft palate and neural tube defects can result from genetic defects or environmental exposures during development, yet very little is known about the effect of chemical exposures on fusion events during human development because of a lack of relevant and robust human in vitro assays of developmental fusion behavior. Given the etiology and prevalence of cleft palate and the relatively simple architecture and composition of the embryonic palate, we sought to develop a three-dimensional culture system that mimics the embryonic palate and could be used to study fusion behavior in vitro using human cells. We engineered size-controlled human Wharton’s Jelly stromal cell (HWJSC) spheroids and established that 7 days of culture in osteogenesis differentiation medium was sufficient to promote an osteogenic phenotype consistent with embryonic palatal mesenchyme. HWJSC spheroids supported the attachment of human epidermal keratinocyte progenitor cells (HPEKp) on the outer spheroid surface likely through deposition of collagens I and IV, fibronectin, and laminin by mesenchymal spheroids. HWJSC spheroids coated in HPEKp cells exhibited fusion behavior in culture, as indicated by the removal of epithelial cells from the seams between spheroids, that was dependent on epidermal growth factor signaling and fibroblast growth factor signaling in agreement with palate fusion literature. The method described here may broadly apply to the generation of three-dimensional epithelial-mesenchymal co-cultures to study developmental fusion events in a format that is amenable to predictive toxicology applications.

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Section: Discussionmentioning

confidence: 99%

Engineering human cell spheroids to model embryonic tissue fusion in vitro

et al. 2017

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“…The images of stained cells were captured using a phase contrast microscope with a digital camera (IM50, Leica, Germany). Cell mineralization was determined by measuring the absorbance of Alizarin Red S-stained cells at 570 nm [ 44 ].…”

Section: Methodsmentioning

confidence: 99%

Dietary Natural N-Acetyl-d-Glucosamine Prevents Bone Loss in Ovariectomized Rat Model of Postmenopausal Osteoporosis

Jiang

Zhang

et al. 2018

Molecules

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Postmenopausal osteoporosis has seriously affected the life quality of elderly women. A natural polymer, chitin, obtained from shells of crab and shrimp, has been widely used in the biomedical field owing to its nontoxicity, biocompatibility, and biodegradability. In this study, natural N-acetyl-d-glucosamine (NAG) was prepared from liquefied chitin. The protective activities of NAG in postmenopausal osteoporosis were evaluated on Sprague Dawley rats and osteoblast-based models. Results showed that oral administration of NAG boosted trabecular bone volume and trabecular numbers. Additionally, the calcium content in the femur and tibia increased, and femoral biomechanical properties improved. Furthermore, NAG supplementation significantly lowered alkaline phosphatase levels and increased calcium content in the serum of ovariectomized rats. In vitro studies showed that NAG markedly promoted cell proliferation and stimulated osteoblast differentiation of mouse calvaria origin MC3T3-E1 cells with increased alkaline phosphatase activity in a concentration-dependent manner. Moreover, NAG effectively protected osteoblasts from oxidative damage induced by hydrogen peroxide. In conclusion, our data provide an additional foundation for dietary supplementation of NAG, which could protect and reverse osteopenia in postmenopausal women.

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“…The main function of this experiment was to quantify the new production of hydroxyapatite (the main component of bone mineralization) by osteoblasts, which allows bone tissue surrounding titanium surfaces to differentiate and mature ( 29 ).…”

Section: Methodsmentioning

confidence: 99%

Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

Velázquez-Cayón

Castillo-Dalí²,

Corcuera-Flores³

et al. 2017

Med Oral

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BackgroundThe study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix deposition, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish.Material and MethodsAn in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apoptosis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours.ResultsA statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group).ConclusionsIn comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone synthesis as a result of the osteoblasts in contact with it. Key words:BMP-2, Cytoskeleton, cell culture, bone matrix, apoptosis, cell viability.

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Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface

Cited by 16 publications

References 27 publications

Engineering human cell spheroids to model embryonic tissue fusion in vitro

Engineering human cell spheroids to model embryonic tissue fusion in vitro

Dietary Natural N-Acetyl-d-Glucosamine Prevents Bone Loss in Ovariectomized Rat Model of Postmenopausal Osteoporosis

Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium

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