Secretory phospholipase A2-IIA overexpressing mice exhibit cyclic alopecia mediated through aberrant hair shaft differentiation and impaired wound healing response

Chovatiya, Gopal; Sarate, Rahul M.; Sunkara, Raghava R.; Gawas, Nilesh P.; Kala, Vineet; Waghmare, Sanjeev K.

doi:10.1038/s41598-017-11830-9

Cited by 7 publications

(8 citation statements)

References 50 publications

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“…6 Bone morphogenetic proteins (BMPs) can inhibit hair follicle development. 7 The growth and development of hair follicles are also regulated by many signal pathways. The Wnt/⊎-catenin signal pathway plays an important role in the growth, development, and differentiation of hair follicles.…”

Section: Introductionmentioning

confidence: 99%

Vitamin A alleviates heat stress‐induced damage to hair follicle development in Rex rabbits

Yue

Liu

et al. 2021

J Sci Food Agric

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BACKGROUND Rex rabbits are important fur rabbits. Heat stress severely reduces the fur quality of Rex rabbits. The aim of this study was to experimentally investigate the effect of dietary vitamin A (VA) addition on hair follicle development and related signal pathways in Rex rabbits under heat stress. RESULTS In the experiment, 90 Rex rabbits were randomly divided into three groups: control group (20–25 °C, fed basic diet), heat stress group (30–34 °C, fed basic diet), and heat stress + VA group (20–25 °C, fed 12 000 IU/kg VA in addition to the basic diet). VA could significantly increase the hair follicle density (P < 0.01), hair length (P < 0.05), and the ratio of secondary to primary hair follicles (P < 0.05). In addition, VA could significantly inhibit the expression of BMP2, BMP4, FGF5, TGF‐β1, and miR‐214 in heat‐stressed Rex rabbits and significantly increase the expression of noggin, IGF1, IGF1R, Wnt10b, CTNNB1, SHH, and miR‐203 and the levels of Wnt10b and p‐β‐catenin; however, there was no significant effect of VA on the expression of EGF and miR‐205. CONCLUSION The dietary addition of VA can increase the hair follicle density and fur quality of heat‐stressed Rex rabbits. Wnt10/β‐catenin, insulin‐like growth factor 1 (IGF1), fibroblast growth factor 5 (FGF5), noggin–BMP, and sonic hedgehog (SHH) signaling were associated with VA regulation under heat stress. It is possible that miR‐205 and miR‐194 contribute to the regulation of Wnt10/β‐catenin and bone morphogenetic protein (BMP) signaling. © 2021 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Vitamin A alleviates heat stress‐induced damage to hair follicle development in Rex rabbits

Yue

Liu

et al. 2021

J Sci Food Agric

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“…For cryosectioning, the skin samples were directly embedded in OCT compound (Tissue-Tek). The embedded skin was cryosectioned at 5 to 10-μm thickness on lysine coated slides and used for immunofluorescence staining as described earlier [2,21,22,37,38]. For the IHC of the tumour tissues, the antigen retrieval was performed by heating the slides in 10 mM sodium citrate (pH 6.0) in a microwave for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…In the skin, sPLA 2 -IIA is predominantly expressed in the proliferating keratinocytes of the basal layer of the epidermis [18,19]. Moreover, overexpression of sPLA 2 -IIA in transgenic mice led to epidermal hyperplasia and alopecia [[20], [21], [22]]. However, how the sPLA 2 -IIA affects the long-term maintenance of HFSC pool and its ultimate fate in the in vivo system is yet to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Context-dependent effect of sPLA2-IIA induced proliferation on murine hair follicle stem cells and human epithelial cancer

et al. 2019

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Background: Tissue stem cells (SCs) and cancer cells proliferation is regulated by many common signalling mechanisms. These mechanisms temporally balance proliferation and differentiation events during normal tissue homeostasis and repair. However, the effect of these aberrant signalling mechanisms on the ultimate fate of SCs and cancer cells remains obscure. Methods: To evaluate the functional effects of Secretory Phospholipase A 2-IIA (sPLA 2-IIA) induced abnormal signalling on normal SCs and cancer cells, we have used K14-sPLA 2-IIA transgenic mice hair follicle stem cells (HFSCs), DMBA/TPA induced mouse skin tumour tissues, human oral squamous cell carcinoma (OSCC) and skin squamous cell carcinoma (SCC) derived cell lines. Findings: Our study demonstrates that sPLA 2-IIA induces rapid proliferation of HFSCs, thereby altering the proliferation dynamics leading to a complete loss of the slow cycling H2BGFP positive HFSCs. Interestingly, in vivo reversion study by JNK inhibition exhibited a significant delay in post depilation hair growth, confirming that sPLA 2-IIA promotes HFSCs proliferation through JNK/c-Jun signalling. In a different cellular context, we showed increased expression of sPLA 2-IIA in human OSCC and mouse skin cancer tissues. Importantly, a xenograft of sPLA 2-IIA knockdown cells of OSCC and SCC cell lines showed a concomitant reduction of tumour volume in NOD-SCID mice and decreased JNK/c-Jun signalling. Interpretation: This study unravels how an increased proliferation induced by a common proliferation inducer (sPLA 2-IIA) alters the fate of normal SCs and cancer cells distinctively through common JNK/c-Jun signalling. Thus, sPLA 2-IIA can be a potential target for various diseases including cancer. Fund: This work was partly supported by the Indian Council of Medical Research (ICMR-3097) and ACTREC (42) grants.

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“…Using HF patch transplantation assay it has been demonstrated that HF stem cells contribute to generating new follicles in wounded mouse skin areas [72]. A complete reduction of HF stem cells in transgenic mice displayed a delay in wound healing after a full-thickness wound in the dorsal area [73]. Similarly, a delay in re-epithelization after the wound is observed in Edaraddcr/cr mice, that have a mutation in HF development [74].…”

Section: Major Functionsmentioning

confidence: 99%

Keratinocyte Stem Cells: Role in Aging

Ansary¹,

Kamiya²,

Ohtsuki³

2022

Keratinocyte Biology - Structure and Function in the Epidermis

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Stem cells located in the skin are responsible for continual regeneration, wound healing, and differentiation of different cell lineages of the skin. The three main locations of skin stem cells are the epidermis, dermis, and hair follicles. The keratinocyte stem cells are located in the epidermal basal layer (the interfollicular stem cells), hair follicle bulge region (the hair follicle stem cells), and sebaceous glands (the sebaceous gland stem cells) and are responsible for the epidermal proliferation, differentiation, and apoptosis. The interfollicular (IF) stem cells are responsible for epidermis regeneration by proliferating basal cells that attach to the underlying basement membrane and with time they exit from the cell cycle, start terminal differentiation, and move upward to form the spinous, the granular, and the stratum corneum layers. The hair follicle (HF) stem cells are responsible for hair regeneration and these stem cells undergo a cycle consists three stages; growth cycles (anagen), degeneration (catagen), and relative resting phase (telogen). The sebaceous gland (SG) stem cells located in between the hair follicle bulge and the gland and are responsible for producing the entire sebaceous gland which secretes oils to moisture our skin. The role of epidermal stem cells is extremely crucial because they produce enormous numbers of keratinocytes over a lifetime to maintain epidermal homeostasis. However, the age-associated changes in the skin; for example; alopecia, reduced hair density, gray or thin hair, reduced wound healing capacity are related to skin stem cells’ decline functionality with age.

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Secretory phospholipase A2-IIA overexpressing mice exhibit cyclic alopecia mediated through aberrant hair shaft differentiation and impaired wound healing response

Cited by 7 publications

References 50 publications

Vitamin A alleviates heat stress‐induced damage to hair follicle development in Rex rabbits

Vitamin A alleviates heat stress‐induced damage to hair follicle development in Rex rabbits

Context-dependent effect of sPLA2-IIA induced proliferation on murine hair follicle stem cells and human epithelial cancer

Keratinocyte Stem Cells: Role in Aging

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