Secretory phosphoprotein 1 secreted by fibroblast-like synoviocytes promotes osteoclasts formation via PI3K/AKT signaling in collagen-induced arthritis

Cai, Xiaoyu; Zheng, Yan; Ren, Fujia; Zhang, Shiwei; Wu, Liquan; Yao, Yao

doi:10.1016/j.biopha.2022.113687

Cited by 15 publications

(6 citation statements)

References 40 publications

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“…Wnt1 is mainly expressed in synovial cells, after the activation of Wnt signaling pathway, the expression of βcatenin increases, which promotes the secretion of inflammatory factors. RA-FLS is activated and induces RA, Cai et al (2022) confirmed that blocking Wnt1/β-catenin signaling pathway and inhibiting TNF-induced migration, invasion and inflammation of RA-FLS cells can effectively alleviate adjuvant arthritis (AA). Some miRNAs can be used as inhibitors of Wnt1/β-catenin signaling pathway to further prevent RA (Miao et al, 2014;Sujitha et al, 2020) showed that berberine could inhibit Wnt1/β-catenin signal transduction through miR-23a activation, thereby improving RA.…”

Section: Wnt1/β-catenin Signaling Pathwaymentioning

confidence: 83%

“…However, the types of arthritis treated with these targets are not completely clear. Targets, β2-adrenergic receptor (ADRB2), AHR, CRP, IRF1, prostaglandin G/H synthase 1 (PTGS1), SPP1 and other targets, exert key effects in the pathogenesis of arthritis, but role of them in the treatment of arthritis with CC has not been confirmed ( Table 3 ) ( Xu et al, 2004 ; Koskela et al, 2012 ; Ko et al, 2013 ; Li et al, 2013 ; Xue et al, 2014 ; Song et al, 2016 ; Trzybulska et al, 2018 ; Bonelli et al, 2019 ; Chu et al, 2019 ; Lin et al, 2019 ; Sun et al, 2020 ; Orecchini et al, 2020 ; Yang et al, 2020 ; Nalesso et al, 2021 ; Tang et al, 2021 ; Xu and Xu, 2021 ; Zou et al, 2021 ; Balasundaram et al, 2022 ; Cai et al, 2022 ; Liu et al, 2022 ; Wang et al, 2022 ; Zhang et al, 2022 ; Zhang et al, 2022 ; Han et al, 2022 ; Man et al, 2022 ; Nguyen et al, 2022 ; Qian et al, 2022 ; Tavallaee et al, 2022 ; Zhuang et al, 2022 ; Afnan et al, 2023 ; Baggio et al, 2023 ; Sun et al, 2023b ; Zhou et al, 2023 ; Fan et al, 2023 ; Huang et al, 2023 ; Ning et al, 2023 ; Qin et al, 2023 ; Skougaard et al, 2023 ; Tu et al, 2023 ; Xie et al, 2023 ; Yan et al, 2023 ; Yu et al, 2023 ; Zhao et al, 2023 ). We have elaborated on the role of targets such as MMP-3, IL-1β, MAPK, IL-6, ADRB2, AHR, CRP, CAV1, CXCL2, SPP1 and other targets in treating arthritis, explored the potential targets and mechanisms of CC in treating arthritis, analyzed the feasibility of CC as an anti-arthritis drug, and provided a theoretical basis for subsequent research.…”

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

“…In collagen induced arthritis, SPP1 secreted by FLSs promotes the formation of osteoclast through PI3K/AKT signals. Regulating the expression of SPP1 gene in FLSs may be a potential method to treat RA bone injury in the joint microenvironment ( Cai et al, 2022 ). Therefore, ADRB2, AHR, CRP, IRF1, PTGS1, SPP1 and other targets can be used as potential targets for CC in the treatment of arthritis, and it is of great significance to explore its role of CC in the treatment of arthritis.…”

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

“…However, the types of arthritis treated with these targets are not completely clear. Targets, β2adrenergic receptor (ADRB2), AHR, CRP, IRF1, prostaglandin G/H synthase 1 (PTGS1), SPP1 and other targets, exert key effects in the pathogenesis of arthritis, but role of them in the treatment of arthritis with CC has not been confirmed (Table 3) (Xu et al, 2004;Koskela et al, 2012;Ko et al, 2013;Li et al, 2013;Xue et al, 2014;Song et al, 2016;Trzybulska et al, 2018;Bonelli et al, 2019;Chu et al, 2019;Lin et al, 2019;Sun et al, 2020;Orecchini et al, 2020;Yang et al, 2020;Nalesso et al, 2021;Tang et al, 2021;Zou et al, 2021;Balasundaram et al, 2022;Cai et al, 2022;Liu et al, 2022;Zhang et al, 2022;Zhang et al, 2022;Han et al, 2022;Man et al, 2022;Nguyen et al, 2022;Qian et al, 2022;Tavallaee et al, 2022;Zhuang et al, 2022;Afnan et al, 2023;Baggio et al, 2023;Sun et al, 2023b;Zhou et al, 2023;Fan et al, 2023;Huang et al, 2023;Ning et al, 2023;Qin et al, 2023;Skougaard et al, 2023;Tu et al, 2023;Xie et al, 2023;…”

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

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Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

Tian

Guo

et al. 2023

Front. Pharmacol.

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Arthritis is a common degenerative disease of joints, which has become a public health problem affecting human health, but its pathogenesis is complex and cannot be eradicated. Coptis chinensis (CC) has a variety of active ingredients, is a natural antibacterial and anti-inflammatory drug. In which, berberine is its main effective ingredient, and has good therapeutic effects on rheumatoid arthritis (RA), osteoarthritis (OA), gouty arthritis (GA). RA, OA and GA are the three most common types of arthritis, but the relevant pathogenesis is not clear. Therefore, molecular mechanism and prevention and treatment of arthritis are the key issues to be paid attention to in clinical practice. In general, berberine, palmatine, coptisine, jatrorrhizine, magnoflorine and jatrorrhizine hydrochloride in CC play the role in treating arthritis by regulating Wnt1/β-catenin and PI3K/AKT/mTOR signaling pathways. In this review, active ingredients, targets and mechanism of CC in the treatment of arthritis were expounded, and we have further explained the potential role of AHR, CAV1, CRP, CXCL2, IRF1, SPP1, and IL-17 signaling pathway in the treatment of arthritis, and to provide a new idea for the clinical treatment of arthritis by CC.

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Section: Wnt1/β-catenin Signaling Pathwaymentioning

confidence: 83%

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

Section: Deep Exploration Based On Potential Therapeutic Targets For ...mentioning

confidence: 99%

See 2 more Smart Citations

Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

Tian

Guo

et al. 2023

Front. Pharmacol.

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“…Xie et al reported that CCL7 promoted the M1 polarization of macrophages [40], which may also be involved in the secretory phenotypic changes in macrophages. Spp1 secreted from SFs in a collagen-induced arthritis model is known to promote osteoclast formation via PI3K/AKT signaling [41]. For proliferating macrophages, the Csf1-Csfr1 signaling was downregulated by treatment with clodronate liposomes.…”

Section: Discussionmentioning

confidence: 99%

Macrophage depletion in inflamed rat knees prevents the activation of synovial mesenchymal stem cells by weakening Nampt and Spp1 signaling

Kodama,

Endo,

Sekiya

2024

Preprint

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Background Macrophages and mesenchymal stem cells (MSCs) engage in crucial interplay during inflammation and have significant roles in tissue regeneration. Synovial MSCs, as key players in joint regeneration, are known to proliferate together with macrophages in synovitis. However, the crosstalk between synovial MSCs and macrophages remains unclear. In this study, we investigated changes in the activation of synovial MSCs in inflamed rat knees following selective depletion of macrophages with clodronate liposomes. Methods Acute inflammation was induced in rat knee joints by injection of carrageenan (day 0). Clodronate liposomes were administered intra-articularly on days 1 and 4 to deplete macrophages, with empty liposomes as a control. Knee joints were collected on day 7 for evaluation by histology, flow cytometry, and colony-forming assays. Concurrently, synovial MSCs were cultured and subjected to proliferation assays, flow cytometry, and chondrogenesis assessments. We also analyzed their crosstalk using single-cell RNA sequencing (scRNA-seq). Results Clodronate liposome treatment significantly reduced CD68-positive macrophage numbers and suppressed synovitis. Immunohistochemistry and flow cytometry showed decreased expression of CD68 (a macrophage marker) and CD44 and CD271 (MSC markers) in the clodronate group, while CD73 expression remained unchanged. The number of colony-forming cells per 1,000 nucleated cells and per gram of synovium was significantly lower in the clodronate group than in the control group. Cultured synovial MSCs from both groups showed comparable proliferation, surface antigen expression, and chondrogenic capacity. scRNA-seq identified seven distinct synovial fibroblast (SF) subsets, with a notable decrease in the Mki67+ SF subset, corresponding to synovial MSCs, in the clodronate group. Clodronate treatment downregulated genes related to extracellular matrix organization and anabolic pathways in Mki67+ SF. Cell-cell communication analysis revealed diminished Nampt and Spp1 signaling interaction between macrophages and Mki67+ SF and diminished Ccl7, Spp1, and Csf1 signaling interaction between Mki67+ SF and macrophages in the clodronate group. Conclusions Macrophage depletion with clodronate liposomes suppressed synovitis and reduced the number and activity of synovial MSCs, highlighting the significance of macrophage-derived Nampt and Spp1 signals in synovial MSC activation. These findings offer potential therapeutic strategies to promote joint tissue regeneration by enhancing beneficial signals between macrophages and synovial MSCs.

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The resistance to anoikis, mediated by Spp1, and the evasion of immune surveillance facilitate the invasion and metastasis of hepatocellular carcinoma

Zhang,

Chen,

et al. 2024

Apoptosis

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Anoikis-Related Genes (ARGs) lead to the organism manifesting resistance to anoikis and are associated with unfavorable prognostic outcomes across various malignancies.Therefore, it is crucial to identify the pivotal target genes related to anoikis in HCC .We found that ARGs were significantly correlated with prognosis and immune responses in HCC. The core gene, SPP1, notably promoted anoikis resistance and metastasis in HCC through both in vivo and in vitro studies. The PI3K-Akt-mTOR pathway played a critical role in anoikis suppression within HCC contexts. Our research unveiled SPP1’s role in enhancing PKCα phosphorylation, which in turn activated the PI3K-Akt-mTOR cascade. Additionally, SPP1 was identified as a key regulator of MDSCs and Tregs migration, directly affecting their immunosuppressive capabilities.These findings indicate that in HCC, SPP1 promoted anoikis resistance and facilitated immune evasion by modulating MDSCs and Tregs. Graphical Abstract

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Secretory phosphoprotein 1 secreted by fibroblast-like synoviocytes promotes osteoclasts formation via PI3K/AKT signaling in collagen-induced arthritis

Cited by 15 publications

References 40 publications

Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

Macrophage depletion in inflamed rat knees prevents the activation of synovial mesenchymal stem cells by weakening Nampt and Spp1 signaling

The resistance to anoikis, mediated by Spp1, and the evasion of immune surveillance facilitate the invasion and metastasis of hepatocellular carcinoma

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