This paper summarizes the management framework, organizing plan, and results of a multi-state, multi-institutional partnership delivering a targeted “train-the-trainer” program addressing food chain security in the southeastern U.S. The partnership provided good agricultural practices (GAPs) and good manufacturing practices (GMPs) –based training to fresh fruit and vegetable (produce) growers and packers throughout the region. Twelve southern states cooperated in this project: Arkansas, Florida, Georgia, Kentucky, Louisiana, Mississippi, North Carolina, Oklahoma, South Carolina, Tennessee, Texas, and Virginia. This 2000–04 work was funded by National Food Safety Initiative grants. Although proposed long before events of 11 Sept. 2001, the project and its results are increasingly relevant since that time. This is because consumer expectations regarding the nation's food supply now include a new security consciousness addressed in this project.