Sedimentation field-flow-fractionation: emergence of a new cell separation methodology

Chianea, Thierry; Assidjo, Nogbou Emmanuel; Cardot, Philippe

doi:10.1016/s0039-9140(99)00335-5

Cited by 71 publications

(52 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With no DEP signal applied, elution profiles corresponded to sedimentation field-flow fractionation 50,51 and were controlled by the balance of sedimentation and hydrodynamic lift forces. Elution of MOLT4 occurred much more slowly than SF295 under these conditions, reflecting both the lower density and greater mechanical flexibility (higher U) of SF295 cells compared with MOLT4.…”

Section: Resultsmentioning

confidence: 99%

Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems

et al. 2013

View full text Add to dashboard Cite

The number of circulating tumor cells (CTCs) found in blood is known to be a prognostic marker for recurrence of primary tumors, however, most current methods for isolating CTCs rely on cell surface markers that are not universally expressed by CTCs. Dielectrophoresis (DEP) can discriminate and manipulate cancer cells in microfluidic systems and has been proposed as a molecular markerindependent approach for isolating CTCs from blood. To investigate the potential applicability of DEP to different cancer types, the dielectric and density properties of the NCI-60 panel of tumor cell types have been measured by dielectrophoretic field-flow fractionation (DEP-FFF) and compared with like properties of the subpopulations of normal peripheral blood cells. We show that all of the NCI-60 cell types, regardless of tissue of origin, exhibit dielectric properties that facilitate their isolation from blood by DEP. Cell types derived from solid tumors that grew in adherent cultures exhibited dielectric properties that were strikingly different from those of peripheral blood cell subpopulations while leukemia-derived lines that grew in non-adherent cultures exhibited dielectric properties that were closer to those of peripheral blood cell types. Our results suggest that DEP methods have wide applicability for the surface-marker independent isolation of viable CTCs from blood as well as for the concentration of leukemia cells from blood.

show abstract

Section: Resultsmentioning

confidence: 99%

Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials.…”

mentioning

confidence: 99%

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

et al. 2005

View full text Add to dashboard Cite

A number of researches have been devoted to the developments of novel separation principles and methodology, because material separation is one of the most important fundamentals in scientific and technological disciplines. In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials. A number of dissolved molecules, from simple ions to macromolecules, can be separated by chromatography or electrophoresis, FFF and electrophoresis being capable of resolving even particles. The dimensions of solutes to be separated by these methods thus range from 10 -10 m to 10 -5 m. This situation may suggest that separation science and technology have been completely matured. However, studying new separation principles is still important, because they can allow the separation based on material properties that have not been utilized for conventional methods, provide higher performance and more efficient separation than existing means, and facilitate the understanding of materials through separation processes.According to chromatographic theories, the intrinsic separation performance of liquid chromatography is restricted in comparison with that of gas chromatography because of much lower solute diffusion in liquid phases. 11However, a liquid flow obviously provides versatile ways for material separation because liquids have higher density and dissolution power than gases, and thus has made possible application to various types of solutes with wide ranges in molecular weights and sizes. In the usual flow separation methods, appropriate chemical (chromatography) or physical (FFF) separation fields are created, which retain solutes to different extents and separate them. A variety of stationary phases for chromatography 2,[12][13][14] and physical forces for FFF [15][16][17][18][19] have been exploited, and have provided different capabilities and selectivity from those performed by conventional separation. In contrast, a separation method that requires neither chemical interactions nor special external fields should also be useful because of its instrumental simplicity. Hydrodynamic chromatography (HDC) is such a case, which allows separation of particles or macromolecules according to their hydrodynamic sizes just by passing them through a narrow channel. [20][21][22][23][24] Particles with larger diameter for example cannot approach the channel wall, and thus flow through the channel faster than the smaller particles, when the laminar flow profile is maintained therein. Although the applicability of this method was very restricted, recent developments of chip technology have brought about a remarkable progress. 23,24 If a capillary of radius a is used for HDC, the time for the elution of a particle of radius rp is given bywhere t0 is the time required ...

show abstract

“…Separation of particles is in general more difficult than that of dissolved molecules, to which various well-established methods such as chromatography, electrophoresis, and solvent extraction are applicable, and thus various separation concepts still deserve attempting. Field flow fractionation (FFF) [1][2][3] is one of the most powerful methods for separating particles with diameters ranging from 10 -3 to 10 2 µm. This method is classified into several categories (e.g.…”

mentioning

confidence: 99%

Elution Control of Microparticles with a Coupled Acoustic-Gravity Field and Orthogonal Laminar Flow

Masudo

Okada

2004

ANAL. SCI.

View full text Add to dashboard Cite

show abstract

Sedimentation field-flow-fractionation: emergence of a new cell separation methodology

Cited by 71 publications

References 47 publications

Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems

Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

Elution Control of Microparticles with a Coupled Acoustic-Gravity Field and Orthogonal Laminar Flow

Contact Info

Product

Resources

About