Seed and pollen transmission of tobacco streak virus in tomato (Lycopersicon esculentum cv. Grosse Lisse)

Abstract

ELISA tests showed that 40-7670 of the seeds of tomato (Lycopersicon esculenturn cv. Grosse Lisse) contained tobacco streak virus (TSV) when either the female (ovule) parent only, the male (pollen) parent only, or both parents were virus infected. When seeds with either or both parents infected were soaked in distilled water overnight and dissected, TSV was found more frequently in the endosperm (40-90%) than the embryo (10-50%), and little or no TSV was present in the seed coat. The seeds which had an infected female (ovule) parent were lighter in weight and had a lower germination rate (3-27%) than seeds which had either an infected male (pollen) parent or two healthy parents (germination rate of 88-96%). Only about 10% of seedlings which had one or both parents infected were found to be infected. TSV was not transmitted to the leaves of healthy tomato plants when flowers were pollinated with pollen from infected tomato plants.

Epidemiology and genetic diversity of Tobacco streak virus and related subgroup 1 ilarviruses

“…While pollination of TSVinfected or healthy plants with TSV-infected pollen can result in infection of the endosperm, embyro and seedlings, transmission to the leaves of healthy mother plants was not detected (Sdoodee and Teakle 1988). Similar to the suggestion of Aparicio et al (1999) for seed transmission of PNRSV, it may be the movement of TSV through the pollen tube (Klose et al 1992) that enables the transmission of TSV to the embryo.…”

“…AgLV was shown to be transmitted by three thrips species and seed transmitted at high rates in some inoculated hosts but not from ageratum, its major alternative host (Greber et al 1991b;Sdoodee and Teakle 1988). However, did later detect seed transmission of TSV-Ag at a low rate (less than 1 %) in seedlings from naturally infected A.…”

“…However, seed transmission between generations of plants (vertical transmission) has also been reported in a range of plant species Sdoodee and Teakle 1988). The rate of reported transmission from naturally infected hosts is variable, including 3% in Melilotus alba (Kaiser et al 1982), 1.4-6% in Phaseolus vulgaris cv.…”

“…When TSV-Ag infected pollen was used to fertilise healthy tomato mother plants, about 11% of the resulting seedlings were TSV-infected (Sdoodee and Teakle 1988). While pollination of TSVinfected or healthy plants with TSV-infected pollen can result in infection of the endosperm, embyro and seedlings, transmission to the leaves of healthy mother plants was not detected (Sdoodee and Teakle 1988).…”

Μελέτη σπορομεταδιδόμενων ιών των χορτοδοτικών ψυχανθών vicia sativa και lathyrus cicera

“…Ο ιός της ασπερμίας της τομάτας {Tomato aspermy cucumovirus, TAV) δε μεταδίδεται με το σπόρο της τομάτας επειδή η μόλυνση επιδρά στη μείωση, προκαλώντας στειρότητα της γύρης και των ωοθηκών (Caldwell, 1952). Επιπλέον, πολλά μολυσμένα φυτά παράγουν μικρότερους σπόρους με χαμηλότερη βλαστικότητα, ενώ τα μολυσμένα σπορόφυτα διακρίνονται από μικρότερη ανταγωνιστική ικανότητα (Hemmati and McLean, 1977• Suteri, 1981• Cooper et al, 1984• Hicks et al, 1986• Sdoodee and Teakle, 1988.…”