Seed dormancy release of Halenia elliptica in response to stratification temperature, duration and soil moisture content

Chen, Da Li; Luo, Xin; Yuan, Zhen; Bai, Meng Jie; Hu, Xiao Wen

doi:10.1186/s12870-020-02560-8

Cited by 19 publications

(17 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seed dormancy is the result of natural evolution of plants in response to adverse environments, but it is not conducive to agricultural production for human beings ( Agrawal et al, 2021 ). Seed dormancy is regulated by temperature, light, hormones and other factors, and affects mRNA splicing, translation and chromatin remodeling ( Chen D. L. et al, 2020 ; Tognacca and Botto, 2021 ). P. cyrtonema seeds began to enter the epicotyl dormancy after the bulb appeared.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

et al. 2022

View full text Add to dashboard Cite

Polygonatum cyrtonema Hua is one of the most useful herbs in traditional Chinese medicine and widely used in medicinal and edible perennial plant. However, the seeds have the characteristics of epicotyl dormancy. In this study, the molecular basis for relieving epicotyl dormancy of P. cyrtonema seeds under exogenous 6-benzylaminopurine (6-BA) treatment was revealed for the first time through transcriptome and metabolomics analysis. We determined the elongation of epicotyl buds as a critical period for dormancy release and found that the content of trans-zeatin, proline, auxin and gibberellin was higher, while flavonoids and arginine were lower in the treatment group. Transcriptome analysis showed that there were significant differences in gene expression in related pathways, and the expression patterns were highly consistent with the change of metabolites in corresponding pathways. Co-expression analysis showed that cytokinin dehydrogenase of P. cyrtonema (PcCKXs) and pelargonidin in flavonoid biosynthesis, as well as L-proline, L-ornithine, and L-citrulline in arginine and proline metabolism form network modules, indicating that they have related regulatory roles. Above all, our findings provide new insight into the exogenous 6-BA relieving epicotyl dormancy of P. cyrtonema seeds.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

et al. 2022

View full text Add to dashboard Cite

show abstract

“…For example, covering the seeds under the litter to ensure effective stratification in winter will facilitate seed germination. On the other hand, collecting seeds to artificially release seed dormancy can prevent animal foraging (Chen et al, 2020). Furthermore, the reintroduction of C. migao seedlings into wild population should be considered an effective method to increase the population of this arbor (Wright et al, 2009).…”

Section: Release Of Seed Dormancy and Natural Regeneration Of The C Migao Populationmentioning

confidence: 99%

Seed Dormancy Release and Germination Requirements of Cinnamomum migao, an Endangered and Rare Woody Plant in Southwest China

et al. 2022

View full text Add to dashboard Cite

Seed dormancy is a complex adaptive trait of plants that are influenced by several physiological and environmental factors. The endangered plant Cinnamomum migao is also known to exhibit seed dormancy and low germination, which may influence its regeneration; however, these characteristics remain unexplored. To our knowledge, this study is the first to examine the type of dormancy and improve the germination percentage of C. migao seeds. We evaluated the structure and characteristics of the embryo and endocarp of C. migao seeds as well as the effects of endogenous inhibitors. Furthermore, we assessed the effects of light, stratification, alternating temperature, and gibberellic acid 3 (GA3) on the dormancy release of these seeds. The embryo was well developed the endocarp was water-permeable, and no obvious mechanical hindrance to germination was observed. However, the endocarp and embryo contained phenols and other germination inhibitors. The seed extracts of C. migao delayed the germination of cabbage and ryegrass seeds, which indicates the presence of endogenous inhibitors. These findings suggest that C. migao seeds exhibit physiological dormancy. Light and an alternating temperature (15/20°C) did not influence germination. However, GA3 pretreatment, alternating temperatures, and warm stratification relieved dormancy. GA3 pretreatment combined with the 15°C stratification treatment was most effective in rapidly releasing the C. migao seed dormancy. Our findings may facilitate the storage and conservation of this endangered plant, which is currently underrepresented in ex situ collections.

show abstract

“…Abscisic acid has been linked to seed dormancy induction and Horticulturae 2021, 7, 490 2 of 12 maintenance, whereas GA is linked to dormancy breaking and germination [10,11]. In addition, cold stratification (CS) is an important environmental element that alleviates seed dormancy [12,13]. Depending on the species, the effective temperature for seed dormancy release during CS ranges from 0 to 10 • C [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, cold stratification (CS) is an important environmental element that alleviates seed dormancy [12,13]. Depending on the species, the effective temperature for seed dormancy release during CS ranges from 0 to 10 • C [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Baek

Kwak

et al. 2021

Horticulturae

View full text Add to dashboard Cite

This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.

show abstract

Seed dormancy release of Halenia elliptica in response to stratification temperature, duration and soil moisture content

Cited by 19 publications

References 34 publications

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

Transcriptomics and metabolomics changes triggered by exogenous 6-benzylaminopurine in relieving epicotyl dormancy of Polygonatum cyrtonema Hua seeds

Seed Dormancy Release and Germination Requirements of Cinnamomum migao, an Endangered and Rare Woody Plant in Southwest China

Non-Deep Physiological Dormancy in Seed and Germination Requirements of Lysimachia coreana Nakai

Contact Info

Product

Resources

About