Seed germination of Egyptian Pancratium maritimum under salinity with regard to cytology, antioxidant and reserve mobilization enzymes, and seed anatomy

Mohamed, Elsayed; Kasem, Ahmed M M A; Farghali, K. A.

doi:10.1016/j.flora.2018.03.011

Cited by 14 publications

(9 citation statements)

References 55 publications

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“…2) evaluate the antioxidant potential of promising isolated strains along with their host plants; 3) determine the phenolic and flavonoid components responsible for antioxidant activity using HPLC analysis, and 4) analyze these components to determine their binding affinities to target myeloperoxidase, an enzyme that catalyzes the formation of reactive intermediates. somniferum, Piper nigrum, and Portulaca oleracea, were collected from Assiut governorate (27 °10′48.4824″N, 31 °11′21.4188″E), Egypt, while Pancratium maritimum mature seeds were collected from the Alexandrian coast of the Mediterranean Sea in Egypt (30 °56′51ʺN, 29 °30′41ʺE) (Mohamed et al, 2018) for the isolation of endophytic fungi. Plant seeds were identified by plant taxonomy staff members based on reference books with species descriptions, keys, and illustrations.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Hassane

Hussien

Abouelela

et al. 2022

Front. Bioeng. Biotechnol.

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Oxidative stress is involved in the pathophysiology of multiple health complications, and it has become a major focus in targeted research fields. As known, black seeds are rich sources of bio-active compounds and widely used to promote human health due to their excellent medicinal and pharmaceutical properties. The present study investigated the antioxidant potency of various black seeds from plants and their derived mycoendophytes, and determined the total phenolic and flavonoid contents in different extracts, followed by characterization of major constituents by HPLC analysis. Finally, in silico docking determined their binding affinities to target myeloperoxidase enzymes. Ten dominant mycoendophytes were isolated from different black seed plants. Three isolates were then selected based on high antiradical potency and further identified by ITS ribosomal gene sequencing. Those isolated were Aspergillus niger TU 62, Chaetomium madrasense AUMC14830, and Rhizopus oryzae AUMC14823. Nigella sativa seeds and their corresponding endophyte A. niger had the highest content of phenolics in their n-butanol extracts (28.50 and 24.43 mg/g), flavonoids (15.02 and 11.45 mg/g), and antioxidant activities (90.48 and 81.48%), respectively, followed by Dodonaea viscosa and Portulaca oleracea along with their mycoendophytic R. oryzae and C. madrasense. Significant positive correlations were found between total phenolics, flavonoids, and the antioxidant activities of different tested extracts. The n-butanol extracts of both black seeds and their derived mycoendophytes showed reasonable IC50 values (0.81–1.44 mg/ml) compared to the control with significant correlations among their phytochemical contents. Overall, seventeen standard phenolics and flavonoids were used, and the compounds were detected in different degrees of existence and concentration in the examined extracts through HPLC analysis. Moreover, the investigation of the molecular simulation results of detected compounds against the myeloperoxidase enzyme revealed that, as a targeted antioxidant, rutin possessed a high affinity (−15.3184 kcal/mol) as an inhibitor. Taken together, the black seeds and their derived mycoendophytes are promising bio-prospects for the broad industrial sector of antioxidants with several valuable potential pharmaceutical and nutritional applications.

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Section: Introductionmentioning

confidence: 99%

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Hassane

Hussien

Abouelela

et al. 2022

Front. Bioeng. Biotechnol.

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“…The results obtained seem to indicate tolerance can come from high levels of esterase, amylase, catalase, and peroxidase activities, and the unique black, spongy, thick seed coat which may act as barrier to saline ionic. In this way, they concluded that this species is a good candidate in their conservation, being able to be used in restoration of coastal area of the Mediterranean Sea [53]. New technologies in study of seeds have helped reveal mechanisms of salinity tolerance in halophytes.…”

Section: Germination Under Saline Conditionsmentioning

confidence: 97%

Adaptation of Halophytes to Different Habitats

Bueno¹

2020

Seed Dormancy and Germination

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In recent years, global climate change has been altering environmental (severe drought, soil salinization, irregular precipitation, etc.), around world, decreasing crop yield and upsetting the balance of ecosystems. Nonetheless, a group of plants known as halophytes have the ability to survive and develop in saline soils (wetlands, deserts or temperate zones), may be used in agriculture as a possible alternative to crops (salt-sensitive), as well as for fodder, energy production, medicinal purposes, and desalination of salt-affected areas (phytoremediation). This chapter provides a comprehensive summary of the adaptive strategies used by the annual and perennial halophytes on ecophysiological perspectives, to survive in diverse habitats. The results show a great diverse strategies, such as heteromorphism, seed banks, dormancy, rapid germination, and recovery capacity, from saline shock, favoring the chances of seed survival, although these mechanisms depend on light, moisture, temperature, and the type of salt, in which seeds germinate. In addition, it has been included some molecular, and biochemical aspects, discovered in last years, that might improve our understanding of physiology of these plants. It can conclude that halophytes may be as a possible alternative to ease pressure on cropping systems, restored lands degraded, or confer stress tolerance trough gene transfer.

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“…They emphasized that the ZhongmuNo.3 cultivar was tolerant to 200 mM NaCl, but Daxiyang was sensitive to salinity at the germination stage. Mohamed et al (2018) revealed that the maximum seed germination percentage was observed in distilled water (100%) followed by 50 mM NaCl (86%) at Pancratium maritimum. They demonstrated that the germination percentage was drastically decreased (50%) in 100 mM NaCl and was completely inhibited at both 200 mM and 400 mM NaCl.…”

Section: Figure 3 Root Relative Growth Index (Rgi) Of Zinnia Cultivarsmentioning

confidence: 98%

“…With the water imbibition, germination stage starts, and different pathways occur (molecular, biochemical, and cellular) in the seed. As a result of these pathways, radicle and hypocotyl protrusion occurs (Nonogaki et al, 2010;Mohamed et al, 2018). Depending on seed characteristics (morphological, genetic, physiological, etc) and environmental effects, seed germination occurs specifically for each plant.…”

Section: Introductionmentioning

confidence: 99%

“…Depending on seed characteristics (morphological, genetic, physiological, etc) and environmental effects, seed germination occurs specifically for each plant. Since salinity affects all pathways during germination, it not only causes germination inhibition but also reduces and delays it (Kaur et al, 1998;Sebei et al, 2007;Mohamed et al, 2018). Seed germination is affected by salinity with osmotic stress and ion toxicity.…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Salt Stress in Zinnia (Zinnia sp.) Cultivars During Seed Germination and at the Early Stages of Seedling Growth

Yasemin

Değer

Köksal

2020

Türkiye Tarımsal Araştırmalar Dergisi

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The Zinnia genus which belongs to the Asteraceae family is an annual, multipurpose ornamental plant. Zinnia plants are cultivated not only in landscape but also as the potted plant and cut flower. One of the most important problems in the world is salinity in soil and water. The aim of this study was to determine the effects of salinity on twenty Zinnia cultivars during seed germination and early seedling growth. The salt was applied by irrigating seeds with 0 and 100 mM salt solutions. Radicle emergence, seed germination, root, hypocotyl and cotyledon lengths, relative growth index of the root, and seedling fresh weight were evaluated. At the end of the study, the radicle emergence reduction was the highest value in Zinnita Rose (52%). The highest reduction rates on seed germination were at Swizzle Cherry-Ivory, Double Zahara Raspberry Ripple (48%), and Double Zahara Yellow (48%). Root lengths of all Zinnia cultivars were dramatically decreased by salt stress. According to weighted ranked evaluation, Dreamland Ivory and Dreamland Coral were more tolerant to salt stress than other Zinnia cultivars. However, in general Zinnia cultivars were relatively sensitive to salt stress at the germination stage.

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Seed germination of Egyptian Pancratium maritimum under salinity with regard to cytology, antioxidant and reserve mobilization enzymes, and seed anatomy

Cited by 14 publications

References 55 publications

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

In Vitro and In Silico Antioxidant Efficiency of Bio-Potent Secondary Metabolites From Different Taxa of Black Seed-Producing Plants and Their Derived Mycoendophytes

Adaptation of Halophytes to Different Habitats

The Effects of Salt Stress in Zinnia (Zinnia sp.) Cultivars During Seed Germination and at the Early Stages of Seedling Growth

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