Seed image analysis provides evidence of taxonomical differentiation within the Lavatera triloba aggregate (Malvaceae)

Bacchetta, Gianluigi; García, Pedro Escobar; Grillo, Oscar; Mascia, Francesco; Venora, Gianfranco

doi:10.1016/j.flora.2011.01.006

Cited by 37 publications

(12 citation statements)

References 17 publications

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“…A cross‐validation procedure was applied to test the performance of the classifiers, as reported by Bacchetta et al . (). Following this approach, statistical classifiers were developed in order to distinguish sample clusters within the five sampled populations and the different substrata and habitat.…”

Section: Methodsmentioning

confidence: 97%

Variability on morphological and ecological seed traits of Limonium avei (De Not.) Brullo & Erben (Plumbaginaceae)

Santo

Mattana

Grillo

et al. 2017

Plant Species Biology

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Limonium avei is an annual species occurring in the salt-marshes and in limited surfaces of rocky areas around the Mediterranean coasts. Seed lots from five populations of this species, along a latitudinal gradient, were analyzed using an image analysis system to detect differences in seed morphology among populations. Germination requirements at constant (5-25 C) and alternating temperatures (25/10 C), both in light and in darkness, were evaluated for all populations, as well as the effect of the calyx removal on final seed germination and its rate. Morpho-colorimetric analysis clearly identified seeds from different populations, habitats and substrates without misattributions among them. The calyx slowed the germination process, influencing both final germination and rate with respect to naked seeds. Seeds from all populations germinated with significantly higher percentages in the light, with respect to those incubated in the darkness, and showed rapid germination (time in days to reach 50% of germination: 0.5 days) at the warmer tested temperature (25 C). High germination (>80%) was also detected for seeds of all the investigated populations, except for those from the Apulian region (South Italy, ca. 60%). Our results highlight that L. avei has a high variability in seed morphology, probably habitat induced, and a fast germination response for all populations. Rapid germination may be an adaptive strategy that allows L. avei seeds to take advantage of transient favorable conditions during the germination stage, to ensure seedling establishment under the unpredictable rainfall pattern in the Mediterranean climate.

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Section: Methodsmentioning

confidence: 97%

Variability on morphological and ecological seed traits of Limonium avei (De Not.) Brullo & Erben (Plumbaginaceae)

Santo

Mattana

Grillo

et al. 2017

Plant Species Biology

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“…This achievement highlights the importance of the introduction of these descriptors, improving the image analysis system previously developed by Grillo et al (2010) in which morphometric features were the first discriminant parameters. Also in Bacchetta, Garc ıa, Grillo, Mascia and Venora (2011), regarding the Lavatera triloba aggregate, the first three parameters with the highest discriminatory power were of morphological type, although colour evaluation was very important in this work for correct seed identification. The present results confirmed the validity of the proposed method for the taxonomic differentiation of Medicago at specific levels, and its identification capability of regional and population groups.…”

Section: Sardiniamentioning

confidence: 87%

Seed image analysis provides evidence of taxonomic differentiation within the Medicago L. sect. Dendrotelis (Fabaceae)

Bianco

Ferrer‐Gallego

Grillo

et al. 2015

Systematics and Biodiversity

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2015): Seed image analysis provides evidence of taxonomic differentiation within the Medicago L. sect. Dendrotelis (Fabaceae), Systematics and Biodiversity,Morpho-colorimetric quantitative variables describing seed size, shape, colour and texture were analysed using image analysis techniques, in order to evaluate the variability among Medicago taxa sect. Dendrotelis and verify the current taxonomic treatment which divides this section into three species: M. arborea L., M. citrina (Font Quer) Greuter and M. strasseri Greuter, Matth€ as & H. Risse. Further comparisons were conducted to discriminate among populations and regions of provenance. Data obtained were statistically analysed applying stepwise Linear Discriminant Analysis method (LDA), recording an overall cross-validated classification performance of 100% at species level. With regard to inter-population comparisons, percentages of correct discrimination above 98% were achieved and high performance was recorded in the discrimination among M. arborea taxa distinguished by region of provenance. For each of these statistical comparisons, the best discriminant variables chosen by the stepwise LDA were related to colour and textural information. Finally, the obtained results confirmed the validity of the proposed method to be highly diagnostic in the statistical assessment of the morpho-colorimetric traits variability of Medicago taxa seeds, both for the taxonomic differentiation at species level and regional and population groups.

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“…This approach is commonly used to classify/identify unknown groups of quantitative and qualitative variables ) and find the combination of predictor variables that simultaneously minimises the within-class distance and maximises the between-class distance, thus achieving maximum class discrimination (Holden et al 2011). A cross-validation procedure was applied to test performance of the classifiers (Bacchetta et al 2011). Following this approach, statistical classifiers were developed to distinguish sample clusters of sampled populations, harvest years and colour of the seed accessions.…”

Section: Discussionmentioning

confidence: 99%

“…Bacchetta et al (2008) from digital images, characterised seeds of wild vascular plants of the Mediterranean Basin, and implemented statistical classifiers to discriminate seeds belonging to different genera and species. Improvements of the applied method and tests on other wild species were then conducted, proving the potential of biometric features measured with image analysis Grillo et al 2010;Bacchetta et al 2011;Orr u et al 2012).…”

Section: Introductionmentioning

confidence: 98%

Morpho‐colorimetric analysis and seed germination of Brassica insularis Moris (Brassicaceae) populations

et al. 2014

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Brassica insularis is a perennial plant growing on both coastal and inland cliffs. Three seed lots from Sardinia were analysed using an image analysis system to detect differences in seed morphology, both within and among populations. Germination requirements at constant (5-25 °C) and alternating temperatures (25/10 °C), both in light and in darkness, were evaluated for all populations. In addition, the effect of a dry after-ripening period (90 days at 25 °C) was also investigated. Morpho-colorimetric analysis clearly identified seeds from different populations and discriminated three chromatic categories for seeds belonging to the Isola dei Cavoli coastal population, but not for the inland Masùa and the coastal Planu Sartu. Inter-population variability was also observed in germination behaviour. B. insularis seeds germinated, with percentages up to 60%, in a wide range of temperatures (5-25 °C), and neither light nor dry after-ripening affected final germination percentages. Moisture content measurements were made for seeds of each colour, but there were no particular differences among colours. Inter-populational variability in germination behaviour may be a survival strategy for species growing under unpredictable environmental conditions, such as under Mediterranean climate, while heteromorphy may be due to independent evolutionary divergence processes of the Isola dei Cavoli population.

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Seed image analysis provides evidence of taxonomical differentiation within the Lavatera triloba aggregate (Malvaceae)

Cited by 37 publications

References 17 publications

Variability on morphological and ecological seed traits of Limonium avei (De Not.) Brullo & Erben (Plumbaginaceae)

Variability on morphological and ecological seed traits of Limonium avei (De Not.) Brullo & Erben (Plumbaginaceae)

Seed image analysis provides evidence of taxonomic differentiation within the Medicago L. sect. Dendrotelis (Fabaceae)

Morpho‐colorimetric analysis and seed germination of Brassica insularis Moris (Brassicaceae) populations

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