Seed Oil Bodies from Gevuina avellana and Madia sativa

Acevedo, Francisca; Rubilar, Mónica; Shene, Carolina; Navarrete, Patricia; Romero, Fernando; Rabert, Claudia; Jolivet, Pascale; Valot, Benoı̂t; Chardot, Thierry

doi:10.1021/jf301390d

Cited by 10 publications

(11 citation statements)

References 45 publications

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“…A putative role has been proposed for this protein as a peroxygenase involved in oxylipin metabolism during biotic and abiotic stress responses in Arabidopsis . The presence of proteins involved in vesicular transport, protein synthesis, and solute transport, in addition to canonical seed OB proteins has also been recently reported . Thus, it appears that different protein classes found in various types of cells and organisms, are also found in seed OBs, which despite the presence of plant‐specific proteins, may harbor a collection of proteins similar to that found in other eukaryotes.…”

Section: Introductionmentioning

confidence: 93%

“…Both salt and alkaline treatments disrupt the electrostatic and hydrophobic interactions between peripheral and integral membrane proteins [31]. The purity of OBs isolated under alkaline conditions and with only three flotation steps, [23,27] was comparable to that of OBs purified using the method of Tzen et al [25]. During most of these procedures, which used relatively harsh conditions, any proteins that are not strongly associated with OBs are certainly removed or degraded, and therefore a lot of the proteins playing a crucial role in OB function and turnover are lost.…”

Section: Preparation Of Highly Pure Obsmentioning

confidence: 99%

“…To date, only a few proteomes of crop seed OBs have been exhaustively described, [23,24,[45][46][47] mostly due to the technical difficulties mentioned above. In all the other studies focused on OB proteins from various species, only the most abundant proteins, such as oleosins, caleosins, and steroleosins were identified.…”

Section: Seed Ob Proteomes: 2012 Censusmentioning

confidence: 99%

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Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

et al. 2013

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Oleaginous seeds store lipids in specialized structures called oil bodies (OBs). These organelles consist of a core of neutral lipids bound by proteins embedded in a phospholipid monolayer. OB proteins are well conserved in plants and have long been grouped into only two categories: structural proteins or enzymes. Recent work, however, which identified other classes of proteins associated with OBs, clearly shows that this classification is obsolete. Proteomics-mediated OB protein identification is facilitated in plants for which the genome is sequenced and annotated. However, it is not clear whether this knowledge can be dependably transposed to less well-characterized plants, including the well-established commercial sources of seed oil as well as the many others being proposed as novel sources for biodiesel, especially in Africa and Asia. Toward an update of the current data available on OB proteins this review discusses (i) the specific difficulties for proteomic studies of organelles; (ii) a 2012 census of the proteins found in seed OBs from various crops; (iii) the oleosin composition of OBs and their role in organelle stability; (iv) PTM of OB proteins as an emerging field of investigation; and finally we describe the emerging model of the OB proteome from oilseed crops.

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Section: Introductionmentioning

confidence: 93%

Section: Preparation Of Highly Pure Obsmentioning

confidence: 99%

Section: Seed Ob Proteomes: 2012 Censusmentioning

confidence: 99%

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Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

et al. 2013

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“…The structure of oil body is similar to liposome [ 34 ]. The liposome can carry drugs into the wound tissue [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

A new nanoscale transdermal drug delivery system: oil body-linked oleosin-hEGF improves skin regeneration to accelerate wound healing

et al. 2018

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BackgroundEpidermal growth factor (EGF) can promote cell proliferation as well as migration, which is feasible in tissue wound healing. Oil bodies have been exploited as an important platform to produce exogenous proteins. The exogenous proteins were expressed in oil bodies from plant seeds. The process can reduce purification steps, thereby significantly reducing the purification cost. Mostly, the diameter of oil body particle ranges between 1.0 and 1.5 µm in the safflower seeds, however, it reduces to 700–1000 nm in the transgenic safflower seeds. The significant reduction of particle size in transgenic seeds is extremely beneficial to skin absorption.ResultsThe diameter of oil body in the transgenic safflower seeds was recorded in the range of 700–1000 nm. The smaller particle size improved their skin absorption. The expression level of oleosin-hEGF-hEGF in T3 transgenic seeds was highest at 69.32 mg/g of seeds. The oil body expressing oleosin-hEGF-hEGF had significant proliferative activity on NIH/3T3 cells and improved skin regeneration thereby accelerating wound healing in rats. The wound coverage rate exceeded 98% after treatment for 14 days with oil body expressing oleosin-hEGF-hEGF, while the saline without EGF group and wild type oil body group both showed less than 80%. The neonatal fibroblast and collagen were found to be increased in the safflower oil body expressing oleosin-hEGF-hEGF treatment group. TGF-β1, bFGF and VEGF were noted as important growth factors in the repair of cutaneous wounds. Their expression level increased after 4 and 7 day treatment, but decreased after 14 days. Therefore, it can promote skin regeneration to accelerate wounds healing.ConclusionsThe expression of oleosin-hEGF-hEGF in T3 transgenic seeds was 80.43 ng/μL oil body. It had significant proliferative activity on NIH/3T3 cells and improved skin regeneration to accelerate wound healing in rats. The expression process of TGF-β1, bFGF and VEGF increased at first and then gradually declined.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0387-5) contains supplementary material, which is available to authorized users.

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“…According to Angelini et al (1997) the average amount of the glyceride oil in Madia sativa seeds was 31.0% with the highest value of 42.0%. Linoleic acid (52.0-72.4%) predominated in fatty acid fraction followed by palmitic and oleic acid (Schmeda-Hirschmann 1995;Angelini et al 1997;Acevedo et al 2012). Despite that, there is scanty information about the content and composition of bioactive compounds such as tocopherols, phospholipids and sterols in Madia sativa seed oil.…”

Section: Introductionmentioning

confidence: 99%

Biologically active components in Madia sativa seed oil

et al. 2017

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Biologically active components in lipids (fatty acids, phospholipids, sterols and tocopherols) from three varieties of seeds introduced in Bulgaria (BGR 457 and BGR 458 with German origin, and BGR 459 with US origin) were investigated. Glyceride oil in the seeds was found to be 36.6, 34.2 and 35.4%, respectively. Total phospholipid content was 2.4, 1.7 and 2.6% and the main classes were phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine. The amount of sterols in the oil was 0.3% for all samples and the major component was β-sitosterol, followed by campesterol and stigmasterol. Total tocopherols in the oils were 768, 795 and 856 mg kg, respectively and α-tocopherol predominated (more than 70.0%). Fatty acid composition of triacylglycerols and sterol esters was also established. Main fatty acids in triacylglycerols were linoleic (47.5-50.5%), oleic (30.2-32.4%) and palmitic acids (13.0-13.5%). The content of saturated fatty acids (palmitic and stearic) in sterol esters (40.1-50.9%) was significantly higher than in triacylglycerols (18.3-19.4%).

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Seed Oil Bodies from Gevuina avellana and Madia sativa

Cited by 10 publications

References 45 publications

Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

A new nanoscale transdermal drug delivery system: oil body-linked oleosin-hEGF improves skin regeneration to accelerate wound healing

Biologically active components in Madia sativa seed oil

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