Seed physiological quality and seedling growth of pea under water and salt stress

Abstract

The aim of this study was to evaluate seed physiological quality and initial pea (Pisum sativum) seedling development at different water and salt potentials. Polyethylene glycol 6000, sodium chloride and potassium chloride solutions with different osmotic potentials (0; -0.2; -0.4; -0.6; -0.8; -1.0, and -1.2 MPa) were used, and the experiment had a 3 × 7 factorial arrangement. The physiological quality and initial seedling growth characteristics (first germination count, final germination count, strong normal seedlings, epicotyl and taproot lengths, epicotyl dry matter, and root dry matter) were evaluated in a completely randomized experimental design, with four repetitions. Decreases in the observed variables showed that the simulated stress conditions negatively affected physiological performance, germination and initial seedling growth. The water and salt stresses induced by the sodium chloride and potassium chloride solutions were greater than the stress induced by polyethylene glycol 6000.

Tolerance of forage pea cultivars to salinity and drought stress during germination and seedling growth

“…Those two cultivars also showed the greatest decrease in germination percentage at -4 bar of drought stress. Drought stress caused by PEG negatively affected germination on pea genotypes in the studies of Okçu et al (2005) and Pereira et al (2020). Similar results have also been obtained for lentil genotypes (Muscolo et al 2013), grass pea varieties (Aslan and Atış, 2018), and some alfalfa genotypes (Özkurt et al, 2018).…”

“…Although the reduction rates of cultivars at -4 bar of drought stress were similar, as with the reduction rates of seedling length, the Töre, Özkaynak, and Ürünlü cultivars had low reduction rates, whereas the Taşkent, Ulubatlı, and Gölyazı cultivars had high ones. Okçu et al (2005), Petrović et al (2016), andPereira et al (2020) all observed a remarkable decrease in the fresh and dry weights of pea seedlings due to the decreased availability of water as doses of NaCl and PEG increased.…”

Antioxidant activity and physiological performance of popcorn seed after saline stress and analysis of seedling images

“…Negative values of osmotic potential delay and reduce the speed and percentage of germination after impairing the sequence of germination events during water absorption (Pereira;Caixeta, 2020). When water is removed below the limit tolerated by the cell, due to drought or a saline environment, the concentration of solutes may increase.…”

“…Studies that simulate water and saline stress in the soil have been performed under laboratory conditions using aqueous solutions of different osmotic potentials to moisten the germination substrates (Pereira;Caixeta, 2020). Such stress factors usually reduce the speed and percentage of seed germination.…”

“…This may cause difficulties in performing the manual measurement of seedling length under normal and stress conditions. Moreover, this is a time-consuming task and often subject to variations between seed analysts (Pereira;Caixeta, 2020). An alternative option of reducing the time taken to complete the manual evaluation, as well as the subjectivity of the test can be the automation of the procedure.…”