Seed Priming and Tolerance to Salt and Water Stress in Divergent Grain Sorghum Genotypes

Abstract

Tolerance to water and salt stress during germination and growth of agricultural species might have superior results when seeds are submitted to priming processes. The objective of the present study was to evaluate the use of hydropriming and hormonal priming with gibberellic acid (GA 3 ) on the tolerance of divergent genotypes of sorghum to salt and water stress during germination and seedling growth. The genotypes analyzed were cultivar BRS 330 and lineage 201420, which are the most and least tolerant to water and salt stress, respectively. Sowing was undertaken under control (no stress), water stress (−0.6 MPa), and salt stress (20 dS·m −1 ) conditions, and the seeds were subjected to the following treatments: control-no immersion; hydropriming-immersion for 2 hours in distilled water; and hormonal priming-immersion in GA 3 solutions, at concentrations of 50, 75, and 100 ppm, for 2 hours. After soaking, the seeds were dried for 24 hours at a temperature of 30˚C ± 2˚C. The variables analyzed were percentage and germination speed index, root and shoot lengths, and root/shoot ratio. The stress conditions analyzed negatively affected the two genotypes; however, the seeds that underwent priming processes improved the performance of the genotypes under salt and water stress conditions, especially when using seeds of the more tolerant genotype (BRS 330) subjected to hormonal priming at 100 ppm GA 3 concentration.

Pengaruh Osmopriming Benih terhadap Pertumbuhan dan Morfofisiologi Tanaman Kangkung Darat (Ipomoea reptans Poir) pada Cekaman Kekeringanngkung Darat (Ipomoea reptans Poir) pada Kondisi Kekeringan

“…Gorzi Gorzi et al (2018) menemukan bahwa priming dengan kombinasi 1 mM SA, 0.5% Fe, 0.5% Zn pada biji Stevia (Stevia rebaudiana) menunjukkan nilai persentase perkecambahan, laju perkecambahan, panjang semaian, indeks vigor semaian, klorofil, aktivitas CAT, dan aktivitas POD maksimum dibandingkan dengan perlakuan lain dan kontrol pada semua tingkat stres kekeringan (0, -3, -6, dan -9 bar). Dampak positif dari priming tersebut juga ditunjukkan pada penelitian Pinheiro et al (2018) yaitu meningkatnya persentase perkecambahan dengan 50 ppm GA 3 serta meningkatnya indeks kecepatan perkecambahan pada biji sorghum genotip BRS-330 dan L-20, pertumbuhan akar, dan keseimbangan rasio akar terhadap pucuk pada genotip BRS-330 dengan 100 ppm GA 3 pada kondisi stres kekeringan.…”

Safeners improve early‐stage chilling‐stress tolerance in sorghum

“…Among the many approaches used to enhance abiotic stress tolerance in plants, exogenous application of agrochemicals such as safeners is considered a new strategy (Abu‐Qare & Duncan, 2002; Bianchi et al., 2020; Dashevskaya et al., 2013; Taylor et al., 2013). Another approach of seed priming using chemicals such as salicylic acid, glycinebetaine, KCl, PEG, GA3 and CaCl 2 has been studied in maize (Farooq et al., 2008; Farooq, Aziz, Basra, Wahid, et al., 2008; Farooq et al., 2008; Farooq, Aziz, Hussain, et al., 2008), and sorghum (Zhang et al., 2015; Pinheiro et al, 2018) to induce abiotic stress tolerance, leading to improved seedling vigour. Herbicide safeners in maize protected seedlings from chilling stress‐induced injury and enhanced dry weight by twofold compared to an untreated control (Kocsy et al., 2001).…”

Sugar Beet Extract Acts as a Natural Bio-Stimulant for Physio-Biochemical analysis of Hordeum Vulgare L. under Induced Salinity Stress

“…Seed priming technique, best pregermination seed treatment has been revealed to boost seedling and yield germination (Nasri et al 2013). The application of SBE as bio-stimulants is boosting speedily in agronomic crops for valuable effects under stressful conditions (Pinheiro et al 2018;Mahdy et al 2020). It was assumed priming of seeds with SBE could lower the damaging effects of salinity stress on seed seedling emergence, germination, and establishment.…”