Seed protein diversity among lentil cultivars

Yüzbaşıoğlu, Ertuğrul; Açık, Leyla; Özcan, Sebahattin

doi:10.1007/s10535-008-0025-4

Cited by 25 publications

(10 citation statements)

References 15 publications

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“…Diverse tools have been used for the evaluation of the genetic diversity in cultivated and wild species, the most widely used being the seed storage proteins together with the microsatellites (Stoilova et al 2006, Mirali et al 2007, Yao et al 2008, Yüzbaşioğlu et al 2008. Furthermore, because the lack of genetic diversity is an important burden on future breeding advances, they could be used to develop new germplasm.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity for morphological traits and seed storage proteins in Spanish rivet wheat

Carmona¹,

Álvarez²,

Caballero³

2010

Biologia plant.

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The objective of the current work was to analyse the variability of high and low molecular mass (HMM and LMM) glutenin subunits, along with some morphological characteristics in sixty Spanish accessions of rivet wheat (Triticum turgidum L. ssp. turgidum). The lines were grouped in sixteen botanical varieties and five additional types, according the morphological criteria. Up to 13 allelic variants (four alleles for the Glu-A1 locus and nine alleles for the Glu-B1 locus) and 34 B-LMMGs patterns were found in the evaluated lines. The current data indicated a clear reduction of morphological variability, along with an asymmetric distribution of the alleles and patterns for seed storage proteins. This polymorphism could be useful for enlarging the genetic background of modern durum wheat.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity for morphological traits and seed storage proteins in Spanish rivet wheat

Carmona¹,

Álvarez²,

Caballero³

2010

Biologia plant.

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“…The dendogram constructed under SDS-PAGE buffer extract grouped C. mannii and C. edulis according to their genus which is in agreement with the taxonomic classification of these species. SDS-PAGE profiles of buffer soluble seed proteins is thus the best method for the study of genetic diversity and has been utilized by other investigators to study genetic diversity in a number of crops (Panda et al, 1986;Panella et al,1993;Sammour, 1991;Javid et al, 2004;Iqbal et al, 2005;Sheidai et al, 2000Sadia et al,2009Ghafoor et al,2002;Koenig et al,1990;Karihaloo et al, 2002;Lioli et al, 2005;Aiken et al, 1998;Yüzbaşıoğlu et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Genetic Diversity within Some Cucurbitaceae Seeds from Nigeria using Electrophoresis

Lukong¹,

Udedi²,

Ezebuo³

et al. 2018

TJANS

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Taxonomic relationships within seven species of Cucurbitaceae; melon seeds were examined at the molecular level with the help of seed storage proteins using electrophoretic methods. Water soluble seed proteins were resolved into 99 and 218 bands respectively under Native-and SDS-PAGE. Similarly 132 and 123 bands respectively were observed for buffer soluble proteins. For water soluble proteins under Native-PAGE, the Jaccard's similarity indexes indicated that Lagenaria siceraria/Cucurbita moschata showed highest similarity index (~96%) while Cucumis sativus/Colocynthis vulgaris showed the lowest similarity index (~35%). For buffer extracts, highest similarity index (~81%) was exhibited by C. sativus/C. maxima while C. sativus /C. mannii showed lowest similarity index (~51%). For water soluble proteins under SDS-PAGE, highest similarity index (~78%) was found between C. maxima /C. vulgaris and the lowest similarity index (34%) was observed between C. sativus /L. siceraria. For buffer extracts, highest similarity index (~98%) was exhibited by C. mannii /C. moschata while C. maxima/C. vulgaris showed the lowest similarity index (~41%). However, from the dendograms constructed, buffer soluble proteins under SDS-PAGE proved to be the best method as it was the only one that grouped C. mannii and C. edulis according to their taxonomic classification.

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“…Also, hybridization is a common incident in this genus, leading to the formation of many interspecific hybrids that increase the taxonomic complexity (Albaladejo et al 2004;Albaladejo et al 2005;Albaladejo and Aparicio, 2007;Yuzbasioglu et al 2008b). Seed storage protein markers have been sucessfully used to resolve taxonomic relationships and characterize cultivated varieties in a number of crop plant species (Igrejas et al 1999;Vladova et al 2000;Jha and Ohri, 2002;Karihaloo et al 2002;Syros et al 2003;Bhargava et al 2005;Cherdouh et al 2005;Alvarez et al 2006;Stoilova et al 2006;Mirali et al 2007;Rout and Chrungoo, 2007;Yuzbasioglu et al 2008a) because they are stable, uniform, reliable, reproducible and largely independent of environmental fluctuations (Ghafoor et al 2002;Panigrahi et al 2007). The taxonomic relationships among species of Phlomis based on seed protein electrophoresis have not been studied so far.…”

Section: *Corresponding Authormentioning

confidence: 99%

Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism

Yüzbaşıoğlu¹,

Dadandi²,

Özcan³

2009

Electron. J. Biotechnol.

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Abbreviations: DTT: dithiothreitolRAPDs: randomly amplified polymorphic DNA SDS-PAGE: sodium dodecyl sulphate polyacrylamide gel electrophoresis TES: TE-saline UPGMA: unweighted pair-group method with arithmetic averagesIn this study, phylogenetic relationships among 39 Phlomis taxa were investigated based on seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). A total of 21 polypeptide bands were scored, of which, 19 were polymorphic among the taxa of the genus Phlomis. A distance matrix was generated from the similarity matrix which was computed by using Jaccard's similarity coefficients, based on polymorphic bands and then an UPGMA tree was established through cluster analysis performed on the distance matrix. Genetic distances ranged from 0.00 to 0.50 within subsection Dendrophlomis; from 0.00 to 0.625 within subsection Gymnophlomis and from 0.00 to 0.769 within subsection Oxyphlomis. The UPGMA tree formed four groups. The topology of the tree is in agreement with the taxonomic view regarding the section Phlomis as it is divided into three subsections as Dendrophlomis, Gymnophlomis and Oxyphlomis based on morphological characters. The grouping pattern of the tree also indicated that subsection Dendrophlomis is more closely related to subsection Gymnophlomis than subsection Oxyphlomis.

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Seed protein diversity among lentil cultivars

Cited by 25 publications

References 15 publications

Genetic diversity for morphological traits and seed storage proteins in Spanish rivet wheat

Genetic diversity for morphological traits and seed storage proteins in Spanish rivet wheat

Evaluation of Genetic Diversity within Some Cucurbitaceae Seeds from Nigeria using Electrophoresis

Estimation of phylogenetic relationships of Phlomis species based on seed protein polymorphism

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