Seed Transmission of Maize Chlorotic Mottle Virus

Jensen, S. G.; Wysong, D. S.; Ball, Ellen M.; Higley, P. M.

doi:10.1094/pd-75-0497

Cited by 66 publications

(48 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transmission rates of HPV in those two seedlots were 0.10 and 0.11%. Thus, our results indicate that HPV is seed transmitted at a low level in the corn lines used in this study, a phenomenon consistent with other viruses infecting corn (4,7,10,16,19,20).…”

Section: Discussionsupporting

confidence: 89%

Seed Transmission of the High Plains virus in Sweet Corn

et al. 2001

View full text Add to dashboard Cite

The High Plains virus (HPV), which infects corn and other cereals, was first found in 1993 in the United States. Research was initiated in 1995 to investigate the potential for seed transmission of HPV. Sweet corn seeds of various cultivars harvested in 1994 to 1996 from 13 fields and research plots in southwestern Idaho, Colorado, and Nebraska were seeded in potting mix in the greenhouse. Leaf samples collected at the three- to six-leaf stage from both symptomatic and asymptomatic plants were tested by enzyme-linked immunosorbent assay (ELISA). Of the 46,600 seeds planted, 38,473 seedlings emerged, and three tested positive by ELISA, exhibited mosaic symptoms, and had the presence of HPV confirmed by an additional test. One of the positive plants was used for successful acquisition and transmission of HPV by the wheat curl mite to Westford barley. The other two plants were used to successfully transfer HPV to other corn plants by vascular puncture inoculation of seed. These results indicate that HPV can be seed transmitted at a very low frequency in sweet corn.

show abstract

Section: Discussionsupporting

confidence: 89%

Seed Transmission of the High Plains virus in Sweet Corn

et al. 2001

View full text Add to dashboard Cite

show abstract

“…The serological detection method is a suitable large-scale detection technique due to its high sensitivity, rapidity, and specificity. It has been widely used to detect and diagnose plant viruses (Jensen et al, 1991;Yu et al, 2005;Wu et al, 2009;Shang et al, 2011). Jensen et al (1991)'s work, a DAS-ELISA for MCMV detection based on the prepared PAbs, was established and the virus was easily detectable in homogenates of infected leaves at a dilution of 1:4 000 (w/v, g/ml).…”

Section: Discussionmentioning

confidence: 99%

“…The results of our study indicate that MCMV has only occurred in Yunnan and Sichuan provinces, indicating that MCMV has not yet spread in other parts of China. Since MCMV can be introduced into corn production areas through seed transmission (Jensen et al, 1991;Zhang et al, 2011), quarantine measures are necessary to prevent the further spread of MCMV in China.…”

Section: Discussionmentioning

confidence: 99%

“…MCMV often induces corn lethal necrosis (CLN) resulting from synergistic interaction between this virus and maize dwarf mosaic virus (MDMV) (Niblett and Clafin, 1978;Goldberg and Brakke, 1987), wheat streak mosaic virus (WSMV) (Scheets, 1998), or sugarcane mosaic potyvirus (SCMV) (Uyemoto et al, 1980), leading to serious yield losses in corn (Uyemoto, 1983;Scheets, 1998;Morales et al, 1999;Xie et al, 2011). Adults of six species of chrysomelid beetle were reported to transmit MCMV under experimental conditions (Nault et al, 1978), and seed transmission was also reported for the virus (Jensen et al, 1991;Zhang et al, 2011). Recently, Dr. Bressan found thrips transmitting MCMV in Hawaii, USA (personal communication, Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Wang

Huan

et al. 2013

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, purified MCMV particles were used as the immunogen to produce monoclonal antibodies (MAbs) and polyclonal antibodies (PAbs). Four murine MAbs (4B8, 8C11, 6F4, and 9G1) against MCMV were obtained through the hybridoma technology. The triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), dot-immunobinding assay (DIBA), and immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) using the MAb 4B8 were then developed for sensitive, specific, and rapid detection of MCMV in fields. MCMV could be detected in infected leaf crude extracts at dilutions of 1:327 680, 1:64 000, and 1:3 276 800 (w/v, g/ml) by TAS-ELISA, DIBA, and IC-RT-PCR, respectively. One hundred and sixty-one maize field samples showing virus-like symptoms and sixty-nine symptomless maize field samples from ten different provinces of China were collected and screened for the presence of MCMV using the established serological methods. A phylogenetic tree was constructed based on the full length CP genes and Chinese MCMV isolates formed one branch with Thailand isolates. The detection results demonstrated that MCMV is one of most prevalent viruses infecting maize in the Yunnan and Sichuan provinces of China.

show abstract

“…MLN causing viruses can be transmitted by a range of insect vectors endemic in East Africa including thrips (Cabanas et al 2013) or chrysomelid beetles for MCMV (Nault 1978) and predominantly aphid species for potyviruses (Brault et al 2010). Both viruses can also be transmitted by seed contamination, mechanism which can contribute to rapid and long-range dissemination of the disease (Jensen 1991;Zhang et al 2011). Management of MLN in East Africa has been hampered by continuous cultivation of maize throughout the year, lack of resistant maize germplasm and the complicated nature of disease spread and development.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of tropical maize inbred lines for resistance to maize lethal necrosis disease

et al. 2017

View full text Add to dashboard Cite

Maize lethal necrosis (MLN) disease is a recent outbreak in eastern Africa and has emerged as a significant threat to maize production in the region. The disease is caused by the co-infection of Maize chlorotic mottle virus and any member of potyviridae family. A total of 28 maize inbred lines with varying levels of tolerance to MLN were crossed in a halfdiallel mating design, and the resulting 340 F 1 crosses and four commercial checks were evaluated under MLN artificial inoculation at Naivasha, Kenya in 2015 and 2016 using an alpha lattice design with two replications. The objectives of the study were to (i) investigate the magnitude of general combining ability variance (r GCA 2 ) and specific combining ability variance (r SCA 2 ) and their interaction with years; (ii) evaluate the efficiencies of GCA based prediction and hybrid performance by means of a cross-validation procedure; (iii) estimate trait correlations in the hybrids; and (iv) identify the MLN tolerant single cross hybrids to be used as female parents for threeway cross hybrids. Results of the combined analysis of variance revealed that both GCA and SCA effects were significant (P \ 0.05) for all traits except for ear rot. For MLN scores at early and late stages, GCA effects were 2.5-3.5 times higher than SCA effects indicating that additive gene action is more important than non-additive gene action. The GCA based prediction efficiency for MLN resistance and grain yield accounted for 67-90% of the variations in the hybrid performance suggesting that GCA-based prediction can be proposed to predict MLN resistance and grain yield prior to field evaluation. Three parents, CKDHL120918, CML550, and CKLTI0227 with significant GCA effects for GY (0.61-1.21; P \ 0.05) were the most resistant to MLN. Hybrids ''CKLTI0227 9 CML550'', ''CKDHL120918 9 CKLTI0138'', and ''CKDHL120918 9 CKLTI0136'' ranked among the best performing hybrids with grain yield of 6.0-6.6 t/ha compared with mean yield of commercial check hybrids (0.6 t/ha). The MLN tolerant inbred lines and single cross hybrids identified in this study could be used to improve MLN tolerance in both public and private sector maize breeding programs in eastern Africa.

show abstract

Seed Transmission of Maize Chlorotic Mottle Virus

Cited by 66 publications

References 0 publications

Seed Transmission of the High Plains virus in Sweet Corn

Seed Transmission of the High Plains virus in Sweet Corn

Monoclonal antibody-based serological methods for maize chlorotic mottle virus detection in China

Genetic analysis of tropical maize inbred lines for resistance to maize lethal necrosis disease

Contact Info

Product

Resources

About