Seed treatments to break seed dormancy and Standardization of viability test procedure in Abrus precatorious

Pallavi, H.M.; Vishwanath, K.; Harish, B.S.; Prashanth, Y.; Thattimani, Manjunath

doi:10.5897/jmpr2013.5102

Cited by 9 publications

(7 citation statements)

References 17 publications

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“…Thus, the above concentration of TZ solution, incubation temperature and time could considered as an optimum for TZ staining in Pinus roxburghii seed and standardized for the future use for the seed viability testing in this species. The results obtained from the present study are configured with the tetrazolium test in Pongamia pinnata [30]; in Moringa oleifera [31]; in Acer caesium [27]; Ulmus wallichiana [27] and in Pinus wallichiana [8]. This is also corroborated with the ISTA rules of tetrazolium test [13].…”

Section: Standardization Of Optimum Conditions For Tz Stainingsupporting

confidence: 83%

Standardization of Quick Seed Viability Protocol for Pinus roxburghii Sarg. Using Tetrazolium Assay

Dhaka,

Thakur,

Kaler

et al. 2024

JABB

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Chir pine (Pinus roxburghii) is one of the important tree species among five indigenous pine species of the Himalayan region and is widely distributed throughout the Himalaya. It is used as a commercial timber, fuel wood and pulpwood and also provide resin for various utilities. For the ecological, silvicultural and economical significance, the regeneration of this species is required for restoration and conservation purposes. Before seed sowing in nursery, there is need to know the actual seed vigour and quality of seeds. Therefore, the present experiment was conducted at Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh of India to standardized a quick seed viability testing protocol using tetrazolium (TZ) and confirmed with laboratory seed germination testing. The tetrazolium test and germination test were conducted with 100 seeds in 4 replications in CRD design as per the procedure devised by ISTA and analyzed with OPSTAT statistical software. After pre-moistening, chir pine seeds were cut longitudinally into two parts away from the radicle end to expose the embryo. The seeds of the species stained with 0.5, 1.0, 1.5 and 2.0 % solution of TZ and incubated at 25, 30 and 35 °C temperature in the darkness for 12, 24 and 36 hours. The result of TZ test showed that the seeds of chir pine soaked in 0.5 % tetrazolium solution at 25 °C temperature for 24 hours found to be the best one for the seed vigour and seed quality testing. For predicting the accurate seed viability, RMS (root mean square) method was used and recognized six staining seed categories distinguished 1 to 6. Seed category 1 denoted as viable seeds and category 6 as non-viable seeds. Result showed that the topographical staining categories 1, 2 and 3 provided least root mean square value and recommended these as viable seed categories for TZ seed viability test. Furthermore, it is also compared with laboratory seed germination test to standardize the seed viability protocol and result in line with the conformity. Overall, the results of the present study fulfilled all the gaps and criteria of quick seed viability testing in chir pine species and standardized protocol could be used for future seed vigour testing within a short period of time for the forest restoration and conservation purpose.

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Section: Standardization Of Optimum Conditions For Tz Stainingsupporting

confidence: 83%

Standardization of Quick Seed Viability Protocol for Pinus roxburghii Sarg. Using Tetrazolium Assay

Dhaka,

Thakur,

Kaler

et al. 2024

JABB

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“…The enhanced seedling vigor may be due to the role of gibberellic acid as a growth hormone which promotes root and shoot development. Gibberellic acid stimulates germination of root and shoots in seeds by weakening tissues barriers such as seed coat, by releasing hydrolytic enzymes hence allowing embryo enlargement (Rouhi et al, 2015;Pallavi et al, 2014;Vieira et al, 2002). It also acts by balancing the concentration of abscisic acid in the seed which is a growth inhibitor thus promoting germination (Viera et al, 2002).…”

Section: Discussmentioning

confidence: 99%

Viability and Dormancy of Rice Seeds after Storage and Pre-Treatment with Dry Heat and Chemical Agents

Mutinda¹,

Muthomi²,

Kimani³

et al. 2017

JAS

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Inappropriate seed packaging and seed dormancy affects seed quality and consequently leads to reduced crop stand, low seedling vigour and reduced yields. The objective of the study was to determine the effect of seed packaging material and seed treatment on viability and dormancy in rice seeds. Effect of packaging material was evaluated by storing freshly harvested seeds of Basmati 370 and BW 196 varieties in polythene, khaki, cheese cloth, polypropylene (Pp) sack and gunny bag sat room temperature and at 6 o C for six months. Effect of seed treatment on dormancy was determined by pre-treatment of seeds with nitric acid at 0.1 M, 0.2 M, 0.5 M, 1 M and 2 M, gibberellic acid (GA 3 ) at 1000 ppm, 2000 ppm and 5000 ppm, and hydrogen peroxide at 0.5 M, 1 M, 2 M, 3 M, 5 M and dry heat at 50 o C for 48 hours. The seeds were subjected to germination test on moist paper and data on percentage germination, seedling length and seedling dry weight were taken. Packaging material had a significant (P ≤ 0.05) effect on germination and vigour. At room temperature, seed stored in polythene bag had the highest germination 88.2% and 57.8% for BW 196 and Basmati 370, respectively. Basmati 370 in khaki bag stored in the cold room had the highest germination 48.3%, while BW 196 stored in polythene had the highest (86.0%). Pre-treatment of seeds reduced dormancy, with variety BW 196 showing the highest germination of up to 67%, while variety Basmati 370 had the lowest germination of up to 40%. Gibberellic acid (GA 3 ) at 5000 ppm was the most effective in breaking seed dormancy resulting in up to 95.7% germination. The results of the study indicated that packaging rice seed in polythene bags for periods not exceeding six months and pre-treatment with GA 3 maintains high seed viability and breaks dormancy, respectively, thus ensuring rapid and uniform germination of vigorous seedlings. This would reduce the period farmers have to wait after harvest before they can plant newly harvested rice seeds.

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“…The inability of walnut seeds to germinate might be due to the hard seed coat. As the scarification treatment given to the seed helped in uptake of water, growth hormones and air which was required for seed germination (Finch-Savage and Leubner-Metzger, 2006;Pallavi et al, 2014). Prechilling stratification might have a significant effect on seed dormancy because, at low temperature, more oxygen is dissolved in water making more oxygen available for the embryo (Young & Young, 1992).…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Pre-Sowing Treatments on Germination of Persian Walnut (Juglans Regia L.) in Rukum (East) District, Nepal

Lamichhane

Thapa

et al. 2021

Turkish JAF Sci.Tech.

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A field-based experiment was carried out in Rukum (East), Nepal, from February to June 2020, to assess the effect of different pre-sowing treatments on germination of Persian walnut. The experiment was carried out in a randomized complete block design with six treatments and four replications. The treatments included hot water treatment, chilling stratification only, cracking + Gibberellic acid (500ppm) followed by chilling stratification, cracking + Gibberellic acid (750ppm) followed by chilling stratification, Gibberellic acid (500ppm) + chilling stratification and Gibberellic acid (750ppm) + chilling stratification. The minimum days for germination (15.75 days) and highest germination (53.25%) were obtained when the combination of cracking with GA3 @ 750 ppm along with chilling stratification was done. The maximum shoot length (34.83 cm) was observed in the combination of cracking with GA3 @ 500 ppm followed by stratification but statistically similar shoot length (34.63 cm) was observed when cracking, application of GA3 @ 750 ppm followed by stratification was done. Cracking, treatment with GA3 @ 500 ppm followed by chilling stratification resulted in the highest shoot fresh weight (11.93 gm) and root fresh weight (10.77 gm) compared to the other treatments used. Thus, cracking along with treatment by GA3 @ 750 ppm followed by chilling stratification could be suggested to the walnut growers for better germination and a better morphological and physiological status of the rootstocks/seedlings.

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Seed treatments to break seed dormancy and Standardization of viability test procedure in Abrus precatorious

Cited by 9 publications

References 17 publications

Standardization of Quick Seed Viability Protocol for Pinus roxburghii Sarg. Using Tetrazolium Assay

Standardization of Quick Seed Viability Protocol for Pinus roxburghii Sarg. Using Tetrazolium Assay

Viability and Dormancy of Rice Seeds after Storage and Pre-Treatment with Dry Heat and Chemical Agents

Effect of Different Pre-Sowing Treatments on Germination of Persian Walnut (Juglans Regia L.) in Rukum (East) District, Nepal

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