Seeding of Human Vascular Cells onto Small Diameter Polyurethane Vascular Grafts

Gulbins, H; Pritisanac, A; Dauner, Martin; Petzold, R; Goldemund, Angelika; Doser, Michael; Meiser, Bruno; Reichart, Bruno

doi:10.1055/s-2005-865916

Cited by 16 publications

(20 citation statements)

References 13 publications

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“…4 Recent procedures involved the pretreatment of prosthesis material with fibrin, collagen, or other matrix molecules that promote cell attachment and retention, but clinical relevance of these biomaterials have to be confirmed by large, human studies. 5,6 Endothelial progenitors alone or cocultured with myofibroblasts and fibrin preparations give also promising results. [7][8][9] Mesenchymal stem cells (MSCs) contribute to angiogenesis in the bone marrow and support hematopoietic cells in adult bone marrow.…”

mentioning

confidence: 94%

Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall

Mirza

Hyvelin

Rochefort

et al. 2008

Journal of Vascular Surgery

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mentioning

confidence: 94%

Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall

Mirza

Hyvelin

Rochefort

et al. 2008

Journal of Vascular Surgery

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“…The great importance of EC coverage of synthetic surfaces in tissue engineering has been shown in vitro [18–21] as well as in animal studies [9,22]. One way to improve endothelialization of cell-repellent surfaces such as PU was nanoupgrading with titaniumcarboxonitride using PACVD technology [12,23].…”

Section: Discussionmentioning

confidence: 99%

In vitro Endothelialization and Platelet Adhesion on Titaniferous Upgraded Polyether and Polycarbonate Polyurethanes

Lehle

Zimmermann

et al. 2014

Materials

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Polycarbonateurethanes (PCU) and polyetherurethanes (PEU) are used for medical devices, however their bio- and haemocompatibility is limited. In this study, the effect of titaniferous upgrading of different polyurethanes on the bio- and haemocompatibility was investigated by endothelial cell (EC) adhesion/proliferation and platelet adhesion (scanning electron microscopy), respectively. There was no EC adhesion/proliferation and only minor platelet adhesion on upgraded and pure PCU (Desmopan). PEUs (Texin 985, Tecothane 1085, Elastollan 1180A) differed in their cyto- and haemocompatibility. While EC adhesion depended on the type of PEU, any proliferative activity was inhibited. Additional titaniferous upgrading of PEU induced EC proliferation and increased metabolic activity. However, adherent ECs were significantly activated. While Texin was highly thrombotic, only small amounts of platelets adhered onto Tecothane and Elastollan. Additional titaniferous upgrading reduced thrombogenicity of Texin, preserved haemocompatibility of Elastollan, and increased platelet activation/aggregation on Tecothane. In conclusion, none of the PUs was cytocompatible; only titaniferous upgrading allowed EC proliferation and metabolism on PEUs. Haemocompatibility depended on the type of PU.

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“…Similarly importants for biocompatibility testing influence the sterilization process of the device with regard to the PUR surface properties 18, 19. The standard sterilization procedure for most pump chambers of artificial hearts is ethylene oxide.…”

Section: Discussionmentioning

confidence: 99%

“…Ethylene oxide is the standard sterilization procedure of PUR made pump chambers. Because Gulbins et al hypothesized that cell attachment was dependent on the sterilization procedure of PUR specimen,18, 19 we tested the cell attachment onto formaldehyde and ethylene oxide sterilized PUR. Our reference was PUR stored in sterilized H 2 O.…”

Section: Methodsmentioning

confidence: 99%

Effect of experimental parameters on the formation of chitosan–poly(acrylic acid) nanofibrous scaffolds and evaluation of their potential application as DNA carrier

Wang

Chen

Kuo

2009

J of Applied Polymer Sci

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Gene delivery from tissue engineering scaffolds has demonstrated the ability to promote gene transfer and stimulate new tissue formation. In this article we report a novel nanofibrous scaffold based on polyelectrolyte complexes as a vehicle for delivery of DNA. When polycation chitosan (CS) was dropped into polyanion poly(acrylic acid) (PAA) suspension and freeze‐dried, CS‐PAA nanofibrous scaffold with diverse microstructure would be formed under different experimental conditions. The nanofiber size was affected by the CS molecular weight, the concentration of CS, the volume ratio of CS to PAA, the reaction temperature, the incubation time, and the final pH of the suspension as well. By using adipic acid as branch promoter, adjusting the pH value of the CS solution to 3, and then dropping CS into the PAA solution at a ratio of 3 : 1, a nanofibrous structure with average diameter 140 nm is obtained after the suspension is freeze‐dried. These nanofibrous scaffolds were nontoxic and can encapsulate plasmid DNA very well. Transgenic expression in human dermal fibroblasts seeded on the nanofibrous scaffolds was significant after 14 days compared to lipofectamine controls. This result indicates that CS‐PAA nanofibrous scaffold have favorable characteristics for nonviral gene delivery to mammalian cell, and have the potential to enhance gene transfer in tissue engineering. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010

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Seeding of Human Vascular Cells onto Small Diameter Polyurethane Vascular Grafts

Abstract: Seeding of a mixed culture consisting of FBs and SMC resulted in an excellent EC adhesion and resistance to shear stress. Cell attachment was better on gamma-sterilized PUVPs compared to Eto-sterilization. The cells obviously maintained their ability to adapt to shear stress.

Cited by 16 publications

References 13 publications

Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall

Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall

In vitro Endothelialization and Platelet Adhesion on Titaniferous Upgraded Polyether and Polycarbonate Polyurethanes

Effect of experimental parameters on the formation of chitosan–poly(acrylic acid) nanofibrous scaffolds and evaluation of their potential application as DNA carrier

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