Seeing biomass recalcitrance through fluorescence

Auxenfans, Thomas; Terryn, Christine; Paës, Gabriel

doi:10.1038/s41598-017-08740-1

Cited by 46 publications

(31 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The negative correlation between saccharification yields and both lignin structure and content was strengthened by the strong negative coefficient obtained for fluorescence intensity, either considering all biomasses (− 0.73) or each biomass separately, with statistically significant values obtained for miscanthus (− 0.99) and poplar (− 0.90). Fluorescence was previously found to be a relevant predictive marker of enzymatic digestibility of steam-exploded biomass samples [ 59 ]. Although the exact mechanism of plant cell wall fluorescence needs to be further investigated, our results indicate that fluorescence intensity can be considered as a good marker of biomass susceptibility to enzymatic hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance

Herbaut

Zoghlami

Habrant

et al. 2018

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

BackgroundBiomass recalcitrance to enzymatic hydrolysis has been assigned to several structural and chemical factors. However, their relative importance remains challenging to evaluate. Three representative biomass species (wheat straw, poplar and miscanthus) were submitted to four standard pretreatments (dilute acid, hot water, ionic liquid and sodium chlorite) in order to generate a set of contrasted samples. A large array of techniques, including wet chemistry analysis, porosity measurements using NMR spectroscopy, electron and fluorescence microscopy, were used in order to determine possible generic factors of biomass recalcitrance.ResultsThe pretreatment conditions selected allowed obtaining samples displaying different susceptibility to enzymatic hydrolysis (from 3 up to 98% of the initial glucose content released after 96 h of saccharification). Generic correlation coefficients were calculated between the measured chemical and structural features and the final saccharification rates. Increases in porosity displayed overall strong positive correlations with saccharification efficiency, but different porosity ranges were concerned depending on the considered biomass. Lignin-related factors displayed highly negative coefficients for all biomasses. Lignin content, which is likely involved in the correlations observed for porosity, was less detrimental to enzymatic hydrolysis than lignin composition. Lignin influence was highlighted by the strong negative correlation with fluorescence intensity which mainly originates from monolignols in mature tissues.ConclusionsOur results provide a better understanding of the factors responsible for biomass recalcitrance that can reasonably be considered as generic. The correlations with specific porosity ranges are biomass species-dependent, meaning that enzymes cocktails with fitted enzyme size are likely to be needed to optimise saccharification depending on the biomass origin. Lignin composition, which probably influences its structure, is the most important parameter to overcome to enhance enzymes access to the polysaccharides. Accordingly, fluorescence intensity was found to be a rapid and simple method to assess recalcitrance after pretreatment.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1053-8) contains supplementary material, which is available to authorised users.

show abstract

Section: Resultsmentioning

confidence: 99%

Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance

Herbaut

Zoghlami

Habrant

et al. 2018

Biotechnol Biofuels

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plant cell walls are autofluorescent materials, containing some endogenous fluorophores, especially aromatic molecules: monolignols in lignin, ferulic, acid and cinnamic acids in hemicellulose (Auxenfans et al, 2017b). Fluorescence can be easily and fastly measured on lignocellulosic samples through spectrofluorimetry.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

Lignocellulosic Biomass: Understanding Recalcitrance and Predicting Hydrolysis

2019

Self Cite

View full text Add to dashboard Cite

Lignocellulosic biomass (LB) is an abundant and renewable resource from plants mainly composed of polysaccharides (cellulose and hemicelluloses) and an aromatic polymer (lignin). LB has a high potential as an alternative to fossil resources to produce second-generation biofuels and biosourced chemicals and materials without compromising global food security. One of the major limitations to LB valorisation is its recalcitrance to enzymatic hydrolysis caused by the heterogeneous multi-scale structure of plant cell walls. Factors affecting LB recalcitrance are strongly interconnected and difficult to dissociate. They can be divided into structural factors (cellulose specific surface area, cellulose crystallinity, degree of polymerization, pore size and volume) and chemical factors (composition and content in lignin, hemicelluloses, acetyl groups). Goal of this review is to propose an up-to-date survey of the relative impact of chemical and structural factors on biomass recalcitrance and of the most advanced techniques to evaluate these factors. Also, recent spectral and water-related measurements accurately predicting hydrolysis are presented. Overall, combination of relevant factors and specific measurements gathering simultaneously structural and chemical information should help to develop robust and efficient LB conversion processes into bioproducts.

show abstract

“…During anther development in wild-type plants, the lignification of the endothecium layer appears after anther stage 10 (Sanders et al, 1999;Wilson et al, 2011). We used confocal laser scanning microscopy to observe the lignin autofluorescence in the endothecium layer of wild type, arf17, and myb108 (Auxenfans et al, 2017). The lignin autofluorescence was observed in the endothecia of wild-type anthers at anther stages 11 and 12.…”

Section: Delayed Lignification In the Endothecium Leads To Defective mentioning

confidence: 99%

AUXIN RESPONSE FACTOR17 Directly Regulates MYB108 for Anther Dehiscence

Wang

Feng

et al. 2019

Plant Physiol.

View full text Add to dashboard Cite

The timely release of mature pollen following anther dehiscence is essential for reproduction in flowering plants. AUXIN RESPONSE FACTOR17 (ARF17) plays a crucial role in pollen wall pattern formation, tapetum development, and auxin signal transduction in anthers. Here, we showed that ARF17 is also involved in anther dehiscence. The Arabidopsis (Arabidopsis thaliana) arf17 mutant exhibits defective endothecium lignification, which leads to defects in anther dehiscence. The expression of MYB108, which encodes a transcription factor important for anther dehiscence, was dramatically downregulated in the flower buds of arf17. Chromatin immunoprecipitation assays and electrophoretic mobility shift assays showed ARF17 directly binds to the MYB108 promoter. In an ARF17-GFP transgenic line, in which ARF17-GFP fully complements the arf17 phenotype, ARF17-GFP was observed in the endothecia at anther stage 11. The GUS signal driven by the MYB108 promoter was also detected in endothecia at late anther stages in transgenic plants expressing promoterMYB108::GUS. Thus, the expression pattern of both ARF17 and MYB108 is consistent with the function of these genes in anther dehiscence. Furthermore, the expression of MYB108 driven by the ARF17 promoter successfully restored the defects in anther dehiscence of arf17. These results demonstrated that ARF17 regulates the expression of MYB108 for anther dehiscence. Together with its function in microcytes and tapeta, ARF17 likely coordinates the development of different sporophytic cell layers in anthers. The ARF17-MYB108 pathway involved in regulating anther dehiscence is also discussed.

show abstract

Seeing biomass recalcitrance through fluorescence

Cited by 46 publications

References 48 publications

Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance

Multimodal analysis of pretreated biomass species highlights generic markers of lignocellulose recalcitrance

Lignocellulosic Biomass: Understanding Recalcitrance and Predicting Hydrolysis

AUXIN RESPONSE FACTOR17 Directly Regulates MYB108 for Anther Dehiscence

Contact Info

Product

Resources

About