Segmental Pancreatic Autotransplantation in the Pig

Shokouh-Amiri, M H; Rahimi‐Saber, S.; Andersen, Henrik Ørbeek

doi:10.1097/00007890-198901000-00010

Cited by 20 publications

(6 citation statements)

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“…In our study, as well as in others20,21, few variations were found in the venous drainage of the pancreas. In only one pig the IMV drained into the splenic vein instead of the SMV.…”

Section: Discussionsupporting

confidence: 81%

“…They found that only 2 pigs exhibited a major pancreatic artery coming from the hepatic artery, but a small arterial branch from the splenic artery was also observed. Shokouh-Amiri MH et al20 had classified the variations in arterial blood supply of the porcine pancreas with three types as they related to the major pancreatic artery (PPA in our study). Nonetheless, they found a PPA arising from the root of the CT.…”

Section: Discussionmentioning

confidence: 93%

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Pig Pancreas Anatomy: Implications for Pancreas Procurement, Preservation, and Islet Isolation

Ferrer¹,

Scott²,

Weegman³

et al. 2008

Transplantation

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Background Islet transplantation is emerging as a treatment option for selected patients with type 1 diabetes. The limited human islet supply from cadavers and poor islet yield and quality remain substantial impediments to progress in the field. Use of porcine islets holds great promise for large-scale application of islet transplantation. Consistent isolation of porcine islets is dependent on advances in pancreas procurement and preservation, and islet isolation requiring detailed knowledge of the porcine pancreatic anatomy. The primary aim of this study was to describe the vascular and ductal anatomy of the porcine pancreas in order to guide and improve organ preservation and enzyme perfusion. Methods Pancreata were removed by en bloc viscerectomy from 65 female Landrace pigs. Results 15% of organs exhibited inconsistent vascular branching from the celiac trunk. All organs had uniform patterns of branching at the superior mesenteric artery. The superior and inferior mesenteric veins (IMV) merged to become the portal vein in all but one case in which the IMV drained into the splenic vein. 97% of pancreata had three lobes: duodenal (DL), connecting (CL), and splenic (SL); 39% demonstrated ductal communication between the CL and the other two lobes; 50% had ductal communication only between the CL and DL; and 11% presented other types of ductal delineation. Conclusions Accounting for the variations in vascular and ductal anatomy, as detailed in this study, will facilitate development of protocols for preservation, optimal enzyme administration, and pancreas distention and digestion, and ultimately lead to substantial improvements in isolation outcomes.

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“…In our study, as well as in others20,21, few variations were found in the venous drainage of the pancreas. In only one pig the IMV drained into the splenic vein instead of the SMV.…”

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 93%

Pig Pancreas Anatomy: Implications for Pancreas Procurement, Preservation, and Islet Isolation

Ferrer¹,

Scott²,

Weegman³

et al. 2008

Transplantation

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“…The PE technique, first described clinically by our group in 1992, 23 was based on experimental work by Shokouh-Amiri et al in a porcine model. 24 This new technique employed a tributary of the superior mesenteric vein to reestablish portal venous drainage and differed substantially from the original reports of portal pancreatic transplants (Calne in 1984, 25 Muhlbacher et al in 1990, 26 and Rosenlof et al in 1992 27 ). In 1993, our group reported that PE pancreas transplantation with Roux limb diversion not only achieves acceptable metabolic control and eliminates hyperinsulinemia, but is also associated with a reduced incidence of postoperative complications.…”

Section: Discussionmentioning

confidence: 97%

Evolution in Pancreas Transplantation Techniques: Simultaneous Kidney-Pancreas Transplantation Using Portal-Enteric Drainage Without Antilymphocyte Induction

et al. 1999

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“…This artery resembles the great pancreatic artery of humans although the major pancreatic artery of pigs does not anastomose with other pancreatic arteries. While the major pancreatic artery is not mentioned in the Nomina Anatomica Veterinaria [13], it has been commonly described in other published reports on the vascular anatomy of the swine pancreas [5, 6, 14–16]. Meticulous dissections allowed the description of an anatomical variation concerning its origin, which was referred to being from the spleen artery (85.5%) or the hepatic artery (14.5%) [14].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Wedged Arterial Injection as a New Technique for Delivery of Experimental Therapeutic Sustances into the Porcine Pancreas

Latorre

Hernández²,

Sun³

et al. 2011

Experimental Diabetes Research

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Objectives. To prospectively evaluate the technical feasibility and efficacy of wedged arterial injection (WAI) as a potential route for experimental selective therapy to the pancreas of healthy pigs. Materials and Methods. Selective angiographies were completed in ten pigs under general anaesthesia. By superselective angiography, the catheter was inserted and wedged into the major pancreatic artery, blocking the blood flow. In order to evaluate the efficacy of the WAI method, a DNA-specific fluorescent dye (Hoechst 33258) was used. Results. Histological study revealed a uniform distribution of the fluorescent dye within the nuclei of the endocrine and exocrine pancreatic cells. Pancreatic and liver enzymes as well as histopathology of the pancreas were normal. Conclusion. WAI is a highly effective minimally invasive methodology to target the porcine pancreas. The findings suggest that WAI may contribute to developing preclinical assays of pancreas gene or cell-transfer therapies in swine model.

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Segmental Pancreatic Autotransplantation in the Pig

Cited by 20 publications

References 0 publications

Pig Pancreas Anatomy: Implications for Pancreas Procurement, Preservation, and Islet Isolation

Pig Pancreas Anatomy: Implications for Pancreas Procurement, Preservation, and Islet Isolation

Evolution in Pancreas Transplantation Techniques: Simultaneous Kidney-Pancreas Transplantation Using Portal-Enteric Drainage Without Antilymphocyte Induction

Evaluation of Wedged Arterial Injection as a New Technique for Delivery of Experimental Therapeutic Sustances into the Porcine Pancreas

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