Segmentation techniques in X-ray images for the analysis of mango stem boring insects

Crocker, T. E.; Thamburaj, Robinson; Nagar, Atulya K.

doi:10.13164/ma.2014.03

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…Several data can be obtained from the evaluation of these images, such as positions related to each other of some developing stages, the spatial orientation of larvae, pupae, adults in plant tissue, or larvae dispersion after the period of the egg hatching. Similar results are reported by Martel and Belanger (1977), Crocker et al (2014), andOrr et al (2015). The feature of some ecological interactions (i.e.…”

Section: Discussionsupporting

confidence: 89%

A conceptualisation of computed tomography outputs in entomological research by step by step displaying trough the CT-based visualization of a wood-boring larvae

Donkó¹,

Petneházy²,

Fajtai³

et al. 2022

APhyt

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The non-invasive diagnostic methods represent a new branch of insect diagnostics, which can provide novel information especially about insects with hidden lifestyle. Computed tomography (CT) is one of the most useful non-destructive techniques allowing for both qualitative and quantitative assessments. The aim of the present study was to attain entomological information through the implementation of CT imaging, hence contributing to the spread of non-invasive imaging in entomological research. Through monitoring the development of wood-dwelling cerambycid larvae in beech branches, we point out some outputs applicable in entomological studies, which originate from CT image post-processing. We present findings on the location, and size of specimens of some hidden arthropods, as well as cavities formed by them, stemming from the maximum and minimum intensity projections, windowing, 3D-reconstruction, or virtual endoscopy, as steps of the imaging. In summary, it is expected that our findings contribute to a wider recognition of the entomological information that can be gathered from these non-invasive imaging techniques.

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Section: Discussionsupporting

confidence: 89%

A conceptualisation of computed tomography outputs in entomological research by step by step displaying trough the CT-based visualization of a wood-boring larvae

Donkó¹,

Petneházy²,

Fajtai³

et al. 2022

APhyt

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“…It happens because their different densities generates the attenuation. In radiographic images of infested seeds, regions that are less dense, such as signs of damage and oviposition, appear darker in color; while denser areas, such as intact seeds, appear in light gray (Crocker, Thamburaj, & Nagar, 2014).…”

Section: Resultsmentioning

confidence: 99%

Determination of Sitotroga cerealella infestation in wheat seeds by radiographic and multispectral images

et al. 2020

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Radiographic and multispectral image analysis have potential to be efficient, objective methods for assessing seed quality and internal insect infestation. The aim of this study was to verify the efficiency of radiographic and multispectral analysis in detecting signs and damage caused by Angoumois grain moth [Sitotroga cerealella (Olivier)] and its different developmental stages in wheat (Triticum aestivum L.) seeds. The experiment was conducted in a completely randomized design with six replications of 50 seeds. The samples were subjected to laboratory-induced infestation and after 5 and 10 d, radiographic and multispectral analysis were conducted. Afterwards, the seeds were immersed in water for 24 h and then sectioned with a cutting blade. The number of seeds with signs of eggs or oviposition, larvae, pupae, adult insects and insect galleries was quantified. The generalized linear models (GLM) methodology was used and the Tukey test (p < .05) was used to compare the means. In general, the radiographic (with or without contrast) and multispectral methods are viable tools to evaluate insect-infested and uninfested wheat seeds. Multispectral analysis was efficient only in identifying eggs on the seed surface and does not detect the presence of larvae and pupae inside the seeds.

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“…A probable explanation for the results obtained in the aforementioned works is that the X-rays form different levels of attenuation of the objects (seed/insect) X-rayed. This attenuation is obtained by reducing the intensity of X-rays as they pass through objects of various densities [25]. Thus, a less dense medium, such as damage and oviposition signs, is displayed in black on the radiographs, while a denser medium, such as undamaged seeds, is highlighted in white.…”

Section: Discussionmentioning

confidence: 99%

The Compared Efficiency of the Traditional Method, Radiography without Contrast and Radiography with Contrast in the Determination of Infestation by Weevil (Sitophilus zeamais) in Maize Seeds

Carvalho

Leite

Carvalho

et al. 2019

Insects

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Technologies that increase safety and efficiency, while facilitating and streamlining the work of seed analysts, are increasingly required by the seed industry. X-ray image analysis is a technique that has been used in the analysis of grain and seeds because it is fast, accurate and non-destructive. The traditional method to verify the presence of insect damage in seeds involves manual cutting of the seeds, which endangers the safety of the analyst and is time-consuming and repetitive work that leads to visual fatigue. The objective of this study was to compared the efficiency of radiographic analysis with and without contrast in the determination of infestation by Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), at different stages of development, in maize seeds, compared to the traditional method required by seed legislation, which consists of cutting and visual evaluation. Seeds were evaluated regarding the presence of eggs/oviposition signs, larvae, pupae, adult insects, insect damage in five infestation periods (5, 18, 33 and 35 days after infestation), while evaluating the total number of seeds infested, comparing the three methods. For characterization of the oviposition stage, the use of contrast was best at all times of infestation. For the larval stage, there was no difference between the evaluation methods; however, at 18 days, larger infestations were observed by the traditional method. At 5 days, the identification of pupae was better by the traditional method and radiography without contrast, while for the identification of adult insects the best method was the use of radiography without contrast. The characterization of the level of infestation with maize weevil damage was best verified using contrast radiography. Radiographic analysis is efficient in the detection of damage caused by S. zeamais in maize seeds. This method of radiographic analysis (with or without contrast) is thus an auxiliary tool to assess the damage and presence of S. zeamais in maize seeds.

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Segmentation techniques in X-ray images for the analysis of mango stem boring insects

Cited by 4 publications

References 2 publications

A conceptualisation of computed tomography outputs in entomological research by step by step displaying trough the CT-based visualization of a wood-boring larvae

A conceptualisation of computed tomography outputs in entomological research by step by step displaying trough the CT-based visualization of a wood-boring larvae

Determination of Sitotroga cerealella infestation in wheat seeds by radiographic and multispectral images

The Compared Efficiency of the Traditional Method, Radiography without Contrast and Radiography with Contrast in the Determination of Infestation by Weevil (Sitophilus zeamais) in Maize Seeds

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