Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism

Brown, Charles R.; Kim, T. S.; Ganga, Z.; Haynes, Kathleen G.; Jong, D. de; Jahn, Molly M.; Paran, Ilan; Jong, Walter De

doi:10.1007/bf02872013

Cited by 75 publications

(70 citation statements)

References 15 publications

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“…However, the molecular details of how this mutation affects carotenoid storage capacity remain to be elucidated. Genetic evidence has identified a major quantitative trait locus (QTL) for tuber carotenoid content on chromosome 3, and there is good evidence that the gene underpinning this QTL is crtRB-2, encoding b-carotene hydroxylase (Brown et al, 2006). Equally clear from the genetic evidence is that other genes are also involved in determining tuber carotenoid content, and it will be of interest to determine whether a tuber carotenoid QTL colocates with the map position of the potato CCD4 gene on chromosome 8.…”

Section: Discussionmentioning

confidence: 99%

“…The Y gene has been mapped to chromosome 3 (Bonierbale et al, 1988), and it has been demonstrated that an allele of b-carotene hydroxylase (bch) was likely to correspond to the Y locus. The genotype at bch accounted for a considerable proportion of the variation in total tuber carotenoid in a range of potato germplasm; however, it is apparent that other genes also contribute to this variation (Brown et al, 2006).…”

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confidence: 99%

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The Metabolic and Developmental Roles of Carotenoid Cleavage Dioxygenase4 from Potato

et al. 2010

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Dakota 58105-5677 (J.C.S.)The factors that regulate storage organ carotenoid content remain to be fully elucidated, despite the nutritional and economic importance of this class of compound. Recent findings suggest that carotenoid pool size is determined, at least in part, by the activity of carotenoid cleavage dioxygenases. The aim of this study was to investigate whether Carotenoid Cleavage Dioxygenase4 (CCD4) activity affects potato (Solanum tuberosum) tuber carotenoid content. Microarray analysis revealed elevated expression of the potato CCD4 gene in mature tubers from white-fleshed cultivars compared with higher carotenoid yellow-fleshed tubers. The expression level of the potato CCD4 gene was down-regulated using an RNA interference (RNAi) approach in stable transgenic lines. Down-regulation in tubers resulted in an increased carotenoid content, 2-to 5-fold higher than in control plants. The increase in carotenoid content was mainly due to elevated violaxanthin content, implying that this carotenoid may act as the in vivo substrate. Although transcript level was also reduced in plant organs other than tubers, such as leaves, stems, and roots , there was no change in carotenoid content in these organs. However, carotenoid levels were elevated in flower petals from RNAi lines. As well as changes in tuber carotenoid content, tubers from RNAi lines exhibited phenotypes such as heat sprouting, formation of chain tubers, and an elongated shape. These results suggest that the product of the CCD4 reaction may be an important factor in tuber heat responses.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The Metabolic and Developmental Roles of Carotenoid Cleavage Dioxygenase4 from Potato

et al. 2010

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“…β-hydroxylase control of β-carotene and β-carotene derived xanthophyll (oxygenated carotenoid) accumulation was previously demonstrated in different plant tissues, such as potato tubers and maize grains (Brown et al, 2006;Yan et al, 2010). In potato tubers, a polymorphism at a β-hydroxylase locus was shown to at least partially account for variation in β-carotene accumulation (Brown et al, 2006).…”

Section: Introductionmentioning

confidence: 98%

“…In potato tubers, a polymorphism at a β-hydroxylase locus was shown to at least partially account for variation in β-carotene accumulation (Brown et al, 2006). In maize, ZmHYD3 displayed expression patterns that correlated with hydroxylated β-carotene derivative (β-xanthophyll) accumulation during endosperm development (Vallabhaneni et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Qin

Zhang²,

Dubcovsky³

et al. 2012

Plant Mol Biol

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Carotenoid β-hydroxylases attach hydroxyl groups to the β-ionone rings (β-rings) of carotenoid substrates, resulting in modified structures and functions of carotenoid molecules. We cloned and characterized two genes (each with three homeologs), HYD1 and HYD2, which encode β-hydroxylases in wheat. The results from bioinformatic and nested degenerate PCR analyses collectively suggest that HYD1 and HYD2 may represent the entire complement of non-heme diiron β-hydroxylases in wheat. The homeologs of wheat HYDs exhibited major β-ring and minor ε-ring hydroxylation activities in carotenoid-accumulating E. coli strains. Distinct expression patterns were observed for different HYD genes and homeologs in vegetative tissues and developing grains of tetraploid and hexaploid wheat, suggesting their functional divergence and differential regulatory control in tissue-, grain development-, and ploidy-specific manners. An intriguing observation was that the expression of HYD1, particularly HYD-B1, reached highest levels at the last stage of tetraploid and hexaploid grain development, suggesting that carotenoids (at least xanthophylls) were still actively synthesized in mature grains. This result challenges the common perception that carotenoids are simply being turned over during wheat grain development after their initial biosynthesis at the early grain development stages. Overall, this improved understanding of carotenoid biosynthetic gene expression and carotenoid metabolism in wheat grains will contribute to the improvement of the nutritional value of wheat grains for human consumption.

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“…For the reactions with TG689 marker, the BCH marker of conserved regions of beta-carotene hydroxylase was added as an internal control for successful PCR (Brown et al 2006).…”

Section: Plant Materialsmentioning

confidence: 99%

Early selection of potato clones with the H1 resistance gene - the relation of nematode resistance to quality characteristics

Milczarek¹,

Przetakiewicz²,

Kamiński³

et al. 2014

CZECH J GENET PLANT

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Milczarek D., Przetakiewicz A., Kamiński P., Flis B. (2014): Early selection of potato clones with the H1 resistance gene -the relation of nematode resistance to quality characteristics. Czech J. Genet. Plant Breed., 50: 278-284.Breeding a new potato cultivar is a long-term process ending with a few elite individuals from initially large populations. Screening for resistance in the seedling and first clonal generations is a cost-effective and efficient way to reduce the time needed to create a new variety. Unlike the phenotypic assessment of resistance to nematodes, marker-assisted selection (MAS) can be applied at early stages of selection. The frequent question among breeders is about the impact of early selection for resistance on the agronomic value of finally selected resistant progeny. The study presents a relationship between the presence of markers TG689 and 57R and some agricultural traits in field grown seedlings and three successive vegetative generations. Both markers are linked to H1 gene, which confers resistance to the golden cyst nematode Globodera rostochiensis. Clones with these markers had higher total tuber and starch yield than those without the markers. A negative relationship between marker presence and quality was not observed.

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Segregation of total carotenoid in high level potato germplasm and its relationship to beta-carotene hydroxylase polymorphism

Cited by 75 publications

References 15 publications

The Metabolic and Developmental Roles of Carotenoid Cleavage Dioxygenase4 from Potato

The Metabolic and Developmental Roles of Carotenoid Cleavage Dioxygenase4 from Potato

Cloning and comparative analysis of carotenoid β-hydroxylase genes provides new insights into carotenoid metabolism in tetraploid (Triticum turgidum ssp. durum) and hexaploid (Triticum aestivum) wheat grains

Early selection of potato clones with the H1 resistance gene - the relation of nematode resistance to quality characteristics

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