Megathrust earthquakes have occurred repeatedly at intervals of 100 to 150 years along the Nankai Trough, situated in the southwest of Japan. Given that it has been 70 years since the last event, the occurrence of the next devastating earthquake is anticipated in the near future. On April 1, 2016, a moderate earthquake (M w 5.9, M JMA 6.5) occurred off the southeastern coast of Mie Prefecture in the source region of the 1944 Tonankai earthquake (M w 8.2). In this study, we investigated the influence of the 2016 earthquake on future megathrust earthquakes. We first determined the hypocenter distributions using a precise velocity structure obtained from seismic surveys in the source region. Using data obtained from the DONET ocean-bottom observation network, we found that this earthquake occurred along the plate boundary fault, which is also believed to have slipped during past megathrust earthquakes. We then performed a preliminary numerical simulation to reproduce the occurrence of a moderate earthquake in the middle of a megathrust earthquake cycle. We used a hierarchical asperity model, in which smaller asperities causing moderate earthquakes are embedded in a hyper-asperity that serves as the source region of megathrust earthquakes. The simulation shows that moderate earthquakes, caused by ruptures of a smaller asperity, occur as a result of shrinkage of strongly coupled areas in the hyper-asperity. This result is consistent with the observation that the hypocenter of the 2016 earthquake was located at the edge of a strongly coupled region along the plate boundary. The simulation also reproduced postseismic slip (afterslip/slow slip) along the plate boundary updip of the hyper-asperity, which is consistent with the observations of slow slip events and very-low-frequency earthquakes after the 2016 earthquake. Thus, the occurrence of the moderate earthquake offshore southeastern Mie Prefecture in the middle of the megathrust earthquake cycle implies that the shrinkage of the strongly coupled area along the plate boundary is occurring as a preparatory process for the next megathrust earthquake in the region.