The shear behavior of joints in precast concrete columns with a new type of column-to-column connection was investigated in this research. Shear tests were performed with 12 precast concrete columns. The influences of the shear key width (0, 70 mm, 100 mm), the diameter of the reinforcement across the joint (0, 16 mm) and the axial compression load ratio (0.3, 0.4, 0.5) on the shear performance of the joint were studied. An expression to give the prediction of the shear capacity of such joints is proposed. The data show that: (1) shear keys have a huge influence on the shear capacity of precast column joints, but the increment of shear capacity due to such influence is limited over a range of shear key widths from 70 to 100 mm; (2) when the axial compression load ratio does not exceed 0.5, a joint’s shear capacity enhances gradually with the load ratio; (3) the expression developed in these experiments outperforms those recommended by four widely-used codes in giving the prediction of the shear capacity of column-to-column connections with shear keys, reinforcement across the joint and grouting.