The effects of the liquid pig manure (LM) used in organic farming on the natural abundance of 15 N and 13 C signatures in plant tissues have not been studied. We hypothesized that application of LM will (1) increase d 15 N of plant tissues due to the high d 15 N of N in LM as compared with soil N or inorganic fertilizer N, and (2) increase d 13 C of plant tissues as a result of high salt concentration in LM that decreases stomatal conductance of plants. To test these hypotheses, variations in the d 15 N and d 13 C of Chinese cabbage (Brassica campestris L.) and chrysanthemum (Chrysanthemum morifolium Ramatuelle) with two different LMs (with d 15 N of +15.6 and +18.2%) applied at two rates (323 and 646 kg N ha -1 for cabbage and 150 and 300 kg N ha -1 for chrysanthemum), or urea (d 15 N = -2.7%) applied at the lower rate above for the respective species, in addition to the control (no N input) were investigated through a 60-day pot experiment. Application of LM significantly increased plant tissue d 15 N (range +9.4 to +14.9%) over the urea (+3.2 to +3.3%) or control (+6.8 to 7.7%) treatments regardless of plant species, strongly reflecting the d 15 N of the N source. Plant tissue d 13 C were not affected by the treatments for cabbage (range À30.8 to À30.2%) or chrysanthemum (À27.3 to À26.8%). However, cabbage dry matter production decreased while its d 13 C increased with increasing rate of LM application or increasing soil salinity (P < 0.05), suggesting that salinity stress caused by high rate of LM application likely decreased stomatal conductance and limited growth of cabbage. Our study expanded the use of the d 15 N technique in N source (organic vs. synthetic fertilizer) identification and suggested that plant tissue d 13 C maybe a sensitive indicator of plant response to salinity stress caused by high LM application rates.Keywords d 13 C Á d 15 N Á Liquid livestock manure Á Organic input Á Salinity Á Synthetic fertilizer
Abbreviations
LMLiquid manure SM Solid manure