Seleção de genótipos de aceroleira assistida por marcadores isoenzimáticos visando à resistência a Meloidogyne incognita raça 2

Rossiter, Jackeline Gadé de Araujo; Musser, Rosimar dos Santos; Martins, Luíza Suely Semen; Pedrosa, Elvira M. R.; Medeiros, Jeane Emilli de

doi:10.1590/s0100-29452008000400036

Cited by 5 publications

(6 citation statements)

References 13 publications

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“…Souza et al (2004) relataram aumentos no conteúdo de prolina e açúcares solúveis totais em plantas de caupi em condições de estresse hídrico. Além disto, a atividade enzimática pode ser reduzida ou estimulada com estresse ambiental e algumas delas, como a peroxidase, podem ser mecanismos de defesa dos hospedeiros a patógenos (Rossiter et al, 2008). A exemplo, Oliveira et al (2012) observaram que a atividade da enzima catálase foi alterada quando a variedade CE-109 estava parasitada com M. incognita.…”

Section: Resultsunclassified

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Sousa¹,

Pedrosa

Rolim

et al. 2015

Rev. bras. eng. agríc. ambient.

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R E S U M OConsiderando a importância do feijoeiro caupi no Nordeste brasileiro, o presente estudo teve, por objetivo, avaliar o crescimento e os aspectos bioquímicos do cultivar EPACE-10 submetido a estresse hídrico e parasitismo de Meloidogyne incognita em condições de casa de vegetação. O delineamento adotado foi de blocos ao acaso em esquema fatorial 2 (com e sem nematoide) × 2 (com e sem estresse hídrico), com seis repetições. O nível de estresse hídrico aplicado (50% da capacidade do campo) diminuiu a reprodução do nematoide; entretanto, o desenvolvimento das plantas aferido pelas biomassas frescas e secas do caule e folhas, número de folhas, índice de área foliar, altura da planta e pela biomassa fresca da raiz, não foi afetado pelo estresse hídrico aplicado. As respostas das enzimas oxidativas ao estresse hídrico e parasitismo de M. incognita foram variáveis. A peroxidase não foi afetada, a catálase foi diminuída pelo parasitismo do nematoide, a ascorbato peroxidade diminuída pelo estresse hídrico e a polifenoloxidase diminuída pelo estresse hídrico e pelo parasitismo de M. incognita. Growth and enzymatic responses of cowpea under water stress and root-knot nematode A B S T R A C TConsidering the importance of cowpea beans in the Northeast of Brazil, the present study aimed to evaluate growing and biochemical aspects of cultivar EPACE-10 subjected to water stress and parasitism of Meloidogyne incognita under greenhouse conditions. The design was a randomized block design in a factorial 2 (with and without nematode) × 2 (with and without water stress), with six replications. The level of applied water stress (50% of the field capacity) reduced the nematode reproduction. However, plant growth measured by fresh and dry biomass of stems and leaves, leaf number, leaf area index, plant height and fresh root biomass was not affected by water stress applied. The responses to water stress and oxidative enzymes and parasitism of M. incognita were variable. Peroxidase was not affected; catalase was decreased by nematode parasitism, ascorbate peroxidase decreased by water stress and polyphenoloxidase decreased by water stress and parasitism of M. incognita. Palavras-chave:Vigna unguiculata Meloidogyne incognita enzimas oxidativas

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Section: Resultsunclassified

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Sousa¹,

Pedrosa

Rolim

et al. 2015

Rev. bras. eng. agríc. ambient.

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“…For the SDB variable, the highest values were observed for genotypes 017-CMF, 036-CMF and 041-CMF, which were classified little or moderately resistant to M. enterolobii. Considering another species of the Meloidogyne genus, [10] did not find a significant difference for the parameters RWR and RWS, in M. emarginata UFRPE AGB genotypes parasited by M. incognita. The evaluation of RWS and RWR may contribute to the selection of tolerant genotypes, to rootknot nematodes, since the absorption and distribution of the nutrients are highly related to the growth rate of the plants, and may be impaired by parasites in the root system [25], but only the observation of developmental characteristics are not sufficient to the determination of resistance or long term tolerance to these parasites.…”

Section: Discussionmentioning

confidence: 58%

“…A survey carried out in several irrigated perimeters in the São Francisco Valley Region, in northwestern Brazil, revealed a high percentage of infected M. emarginata trees, raising the concern that this phytonematode may turn the cultivation unfeasible, as it has happened to guava orchards in that same region [7][8][9]. Genotypes with resistance or tolerance to phytonematodes may be used as rootstocks, as a low cost and sustainable alternative to chemical control methods and, can easily be adopted by growers without environmental and sanitary risks [9][10][11]. The use of resistant rootstocks could provide effective control and significantly reduce the damages caused by M. enterolobii, allowing the recovery of infested areas [12].…”

Section: Introductionmentioning

confidence: 99%

Resistance of Malpighia emarginata DC. Genotypes to Meloidogyne enterolobii Parasitism

Silva

Musser

Martins

et al. 2019

JEAI

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Aims: Considering the inexistence of M. emarginata cultivars resistant to M. enterolobii available for cultivation, and the scarcity of information about the severity of its parasitism in M. emarginata, the present study investigated the response of genotypes from the active germplasm bank of Universidade Federal Rural de Pernambuco to M. enterolobii parasitism, aiming the selection of resistant genotypes for use as rootstocks for commercial varieties. Study Design: The experimental design was completely randomized, with 21 genotypes and one independent matrix (control), with six replicates each. The experimental unit was represented by one plant per plot. Place and Duration of Study: Department of Agronomy, Universidade Federal Rural de Pernambuco – UFRPE - Brazil between June 2013 and July 2014. Methods: In the experiment, completely randomized design was adopted, with 21 genotypes from the AGB and one as a control for susceptibility. The M. emarginata cuttings were inoculated with 10,000 nematode eggs, and after 150 days, they were evaluated for the following parameters: Egg mass index, gall index, reproduction factor, number of eggs per gram of root, number of eggs per root system. Results: Twenty out of the twenty-two genotypes analyzed were susceptible. The genotypes 021-CMF and 037-CMF were considered resistant. To our knowledge, this is the first identification of M. emarginata genotypes resistant to M. enterolobii. Conclusions: These results are of great importance for the breeding and cultivation of the species since these two genotypes can be indicated for use as rootstocks and for breeding programs aimed at transferring resistance to other cultivars with desirable production characteristics that are susceptible to the phytonematode.

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“…isto devido à existência de massas de ovos internas no sistema radicular. a suscetibilidade da aceroleira a M. enterolobii também foi relatada em pesquisas anteriores, demonstrando a dificuldade no encontro de plantas desta espécie resistentes a este nematoide, assim como a outras espécies de nematoide das galhas (SilVa; KRaSUSKi, 2012;RoSSiTeR et al, 2008;GoMeS et al, 2000). na Venezuela, no entanto, castellano et al (2011) relataram a resistência da cultivar cm2 a M.enterolobii , com FR igual a 0,4; e reação de tolerância na cultivar cs1 (FR=5,6), entre outras sete cultivares de aceroleira estudadas.…”

Section: Reaction In Barbados Cherry (Malpighia Emarginata Dc)unclassified

Reação de aceroleira (Malpighia emarginata D.C.) à Meloidogyne enterolobii

Cavichioli¹,

Garcia²,

Brida

et al. 2014

Rev. Bras. Frutic.

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resumo-o Brasil é o maior produtor, consumidor e exportador de acerola (Malpighia emarginata d.c.) do mundo. Por conter altos teores de vitamina c, tornou-se uma fruta altamente requisitada no mercado mundial para o preparo de sucos e no consumo in natura. nos últimos anos, as lavouras desta fruta vêm apresentando um decréscimo nas produções em razão da ocorrência de nematoides de galhas (Meloidogyne spp.), um dos principais problemas que afetam a cultura. o objetivo deste trabalho foi avaliar a resistência de genótipos de aceroleira frente à Meloidogyne enterolobii. o experimento foi conduzido em casa de vegetação do departamento de Proteção Vegetal, da Faculdade de ciências agronômicas/UneSP -câmpus de Botucatu (SP). Foram utilizados cinco clones: cereja-Brs-236; Fruta cor-Brs-238; Roxinha-Brs-237; Mirandópolis; Japi, e três variedades: okinawa; olivier e Waldy-caTi. cada planta foi inoculada com 2.500 ovos e eventuais juvenis de segundo estádio (Pi) de M. enterolobii. após 60 dias, a parte área de cada planta foi descartada, e o sistema radicular lavado, submetido à coloração com floxina-B e examinado para a obtenção dos índices de galhas (iG) e massa de ovos (iMo), e processados pelo método de trituração em liquidificador, peneiramento e centrifugação com sacarose para a obtenção do número total de ovos (Pf), que foi utilizado para o cálculo do fator de reprodução (Pf/Pi). Todos os clones e as variedades foram considerados suscetíveis à Meloidogyne enterolobii apresentando os fatores de reprodução variando de 4,1 a 18,3. termos de indexação: acerola, nematoide de galha, resistência. reactIon In BarBados cherry (Malpighia emarginata d.c.)to Meloidogyne enterolobii aBstract-Brazil is the biggest producer, consumer and exporter of acerola (Malpighia emarginata d.c.). it has become a highly requested fruit in the world market for preparing juices and consumption in nature because it contains high levels of vitamin c. nowadays brazilian acerola orchards have shown decrease in production due to the occurrence of root-knot nematodes, one of the main problem affecting the culture. The aim of this study was to evaluate the resistance of acerola genotypes to Meloidogyne enterolobii. The experiment was conducted in greenhouse of the department of Plant Protection of agronomic Science college -Fca/UneSP -Botucatu, SP. Five clones, cherry-Brs-236; Fruit color-Brs-238; Roxinha-Brs-237; Mirandópolis; Japi, and three varieties, okinawa; olivier and Waldy-caTi, were studied. each plant was inoculated with 2,500 M. enterolobii eggs and second stage juveniles (Pi). after 60 days the roots of each plant was washed, staining with phloxine-B and examined for obtaining gall and egg mass indices (Gi; eMi), and processed by blender, sieving and centrifugation method to obtain the total number of eggs (Pf), which was used to calculate the reproduction factor (Pf / Pi). all acerola clones and varieties were considered susceptible to Meloidogyne enterolobii, with RF ranging from 4.1 to 18.3. Index terms: acerola, root-knot nematode, re...

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Seleção de genótipos de aceroleira assistida por marcadores isoenzimáticos visando à resistência a Meloidogyne incognita raça 2

Cited by 5 publications

References 13 publications

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Crescimento e respostas enzimáticas do feijoeiro caupi sob estresse hídrico e nematoide de galhas

Resistance of Malpighia emarginata DC. Genotypes to Meloidogyne enterolobii Parasitism

Reação de aceroleira (Malpighia emarginata D.C.) à Meloidogyne enterolobii

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