Seleção de isolados de Metarhizium anisopliae (Metsch.) sorok. contra a cigarrinha da raiz da cana-de-açúcar Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) em laboratório

Abstract

Screening of Metarhizium Anisopliae (Metsch.) Sorok. Strains against the Sugarcane Root Spittlebug Mahanarva Fimbriolata (Stål) (Hemiptera: Cercopidae) in Laboratory ABSTRACT-The accumulated mulch on the soil resultant from the mechanized cutting of sugarcane promotes favorable temperature and moist to Mahanarva fimbriolata (Stål), a pest that causes serious problems due to its widespread occurrence and high populations. Seventy nine isolates of Metarhizium anisopliae (Metsch.) Sorok. from different hosts and regions were tested for their pathogenicity. Ten spitlebugs were put in petri dishes (9 cm of diameter) containing sugarcane leaves sprayed with 1 ml of a suspension containing 5 x 10 7 conidia/ml. Five replicates were used per treatment. The petri dishes were kept at 25 ± 1°C, 70 ± 10% RH and 12h photophase. Insects were monitored daily to calculate total cumulative mortality, as well as corrected and confirmed mortality for each treatment. The best tested isolates were: IBCB 348, IBCB 351, IBCB 363, IBCB 408, IBCB 410, IBCB 418, IBCB 425 and IBCB 482, which showed confirmed mortality equal or higher than 70%, six days after spraying.

Characterization and activity of entomopathogenic fungi isolates against “paraguay tea ampul” (Gyropsylla spegazziniana) (Lizer & Trelles) (Hemiptera: Psyllidae)

“…The ability of the fungi to develop the complete cycle is different for each insect, and low percentages of confirmed mortality have frequently been observed, as in studies evaluating the activity of different entomopathogenic fungi against Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) and Collaria scenica (Hemiptera: Miridae) (LOUREIRO et al, 2005;BARBOZA et al, 2011), whereas in a study with Tetraleurodes acaciae (Quaintance) (Insecta, Hemiptera: Aleyrodidae) have found no signs of death caused by fungi in the targeted insects (VILLACARLOS et al, 2003).…”

Control of Mahanarva fimbriolata (Stl) (Hemiptera: Cercopidae) with entomopathogenic fungus and insecticides using two sampling methods on sugarcane fields

“…Coastal sprayers calibrated for a flow rate of 150 L ha −1 (Mendonça, 2005) were used and the insecticide was directed at the stump bases so that 30% of the spray volume reached the stems and 70% reached the sugarcane plant roots (Loureiro et al, 2005). The surfactant Tween ® (0.01% polysorbate 80) was used to treat the fungal suspensions.…”

“…This indicates that the entomopathogenic fungi and insecticides may have a protracted effect in the field (Dinardo-Miranda et al, 2004a;Loureiro et al, 2005;Tiago et al, 2012). However, although generalizations cannot be made, the cumulative effect is controlled by the weather, the active constituent of the insecticide, the chemical mode of action, and the M. anisopliae isolated to control M. fimbriolata (Dinardo-Miranda et al, 2004a;Loureiro et al, 2005). On other hand, the genetic constitution of the pest population (Quinelato et al, 2012), adaptations and mechanisms of insect resistance (Dubovskiy et al, 2013) may influence the efficiency of the control techniques.…”

“…In order to reduce the M. fimbriolata populations (Dinardo-Miranda et al, 2004a, b;Loureiro et al, 2005;Cuarán et al, 2012) insecticides and entomopathogenic fungi like Metarhizium anisopliae (Metschnikoff, 1879;Sorokin, 1883) (Hypocreales: Clavicipitaceae) are used to protect the sugarcane plantations. Control of the spittlebug begins with monitoring the pest, immediately after the first spring rains (Almeida et al, 2007).…”

Alteração no método de amostragem de Mahanarva fimbriolata (Stål, 1854) (Hem.: Cercopidae) e avaliação da eficiência de Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1883 (Hyp.: Clavicipitaceae)

“…Como a maioria das variedades de interesse comercial são suscetíveis às injúrias de ninfas e adultos de M. fimbriolata, pesquisas vêm sendo conduzidas com intuito de avaliar a eficiência de inseticidas e isolados do fungo Metarhizium anisopliae (Metschnikoff, 1879) Sorokin, 1883 (Hypocreales: Clavicipitaceae) para o controle deste inseto (alMeiDa et al, 2003;loureiro et al, 2005;MenDonça, 2005;alMeiDa et al, 2007). Os estudos contribuíram para a implementação de um programa de controle da M. fimbriolata utilizando M. anisopliae e produtos fitossanitários químicos (DinarDo-MiranDa et al, 2004).…”