Seleção de marcadores ISSR e diversidade genética em uma população de Elaeis guineensis

Chagas, Kyvia Pontes Teixeira das; Sousa, Rodrigo Ferreira de; Fajardo, Cristiane Gouvêa; Vieira, Fábio de Almeida

doi:10.5039/agraria.v10i1a5133

Cited by 16 publications

(20 citation statements)

References 13 publications

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“…As a management strategy, these results may support the implementation of subsidies for the genetic conservation of the species in extractive communities. Chagas et al (2015) have suggested that the reduction of the population size of Elaeis guineensis Jacq. (Arecaceae) is due to a loss of habitat and increased agricultural activities at the study site.…”

Section: Genetic Bottlenecksmentioning

confidence: 99%

“…As such, molecular markers are fundamental in analyzing patterns of SGS, characterizing natural plant populations, and estimating gene diversity, inbreeding, gene flow, and degree of kinship (Vieira and Carvalho, 2008;Gonçalves et al, 2010). Of the molecular markers available, ISSR (inter-simple sequence repeat) markers are commonly used in population genetic studies due to their ease of handling, the production of significant polymorphism, short turnaround time for results, and lower cost when compared to other markers Chagas et al, 2015). As such, several studies have used ISSR markers to evaluate the SGS of plant populations (Melo Júnior et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Research Article Anthropization as a determinant factor in the genetic structure of Copernicia prunifera (Arecaceae).

et al. 2017

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ABSTRACT. This study uses ISSR molecular markers to characterize the demographic pattern, and spatial genetic structure (SGS) at different life stages of development (cohorts) in a natural population of Copernicia prunifera in the Rio Grande do Norte State, Brazil. All individuals were sampled and georeferenced in a 0.55-ha plot. The demographic analyses showed a clustered pattern in the first-distance classes and a random or segregated pattern at higher distance classes for all cohorts. Among the three studied life stages, juveniles showed the greatest value for Nei's genetic diversity index (H E = 0.369), while the lowest was found among reproductive adults (H E = 0.341). Greater genetic differentiation was found within life stages (98.61%) than among stages (1.39%). The total population (N = 161) showed positive and significant kinship in the first-distance class (12.3 m). The juveniles showed significant kinship up to 10.5 m. Non-reproductive adults had a positive kinship in the first-distance class (11.0 m) and a random distribution of genotypes in the remaining classes, while reproductive adults showed a random spatial distribution of genotypes. Tests for genetic bottleneck showed that the number of loci with excess observed heterozygosity was greater than expected. The SGS results reflected the restricted seed dispersion of the species, and the genetic bottleneck reflected the reduction of genotypes as a result of the anthropization of C. prunifera natural environments.

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Section: Genetic Bottlenecksmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Research Article Anthropization as a determinant factor in the genetic structure of Copernicia prunifera (Arecaceae).

et al. 2017

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“…Devido à herança dominante, a presença do fragmento (banda) pode representar o homozigoto dominante ou o heterozigoto, enquanto a ausência pode ser infligida o estado homozigoto recessivo, à ocorrência de inserções/deleções, ou à adição ou perda de um sítio de reconhecimento do primer (Zietkiewicz et al, 1994). Apesar do uso contínuo de marcadores ISSR (Brandão et al, 2011;Duarte et al, 2015;Melo Junior et al, 2015), ainda são escassos os estudos envolvendo a metodologia de seleção e indicação do número mínimo de locos para uso na genética de populações (Chagas et al, 2015;Fajardo et al, 2014;Mendonça et al, 2013).…”

Section: Introductionunclassified

Diversidade genética de Copernicia prunifera com o uso de marcadores moleculares ISSR

Vieira

Sousa

Silva

et al. 2015

Agraria

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RESUMOO objetivo desse estudo foi selecionar primers ISSR (Inter Simple Sequence Repeat) para estudos da estrutura genética, assim como quantificar a variabilidade genética de uma população natural da C. prunifera. (UBC 841). O número ótimo de locos para se estimar confiavelmente a diversidade genética para esse trabalho foi de 76 locos. Foi encontrada alta diversidade genética, com o número de alelos observados (na = 2,00), alelos efetivos (ne = 1,46), índice de diversidade de Nei (He = 0,28) e índice de Shannon (Ho = 0,44). O valor da coancestria se manteve dentro do intervalo de confiança (P > 0,05), indicando ausência de estrutura genética espacial. Os marcadores ISSR se mostraram eficientes na caracterização genotípica dos indivíduos de carnaúba, servindo como subsídio para planos de manejo e conservação da espécie.Palavras-chave: Arecaceae, carnaúba, nordeste do Brasil, variação genética Genetic diversity of Copernicia prunifera using ISSR molecular markers ABSTRACTThe aim of this study was to select primers ISSR (Inter Simple Sequence Repeat) for studies of the genetic structure, and to quantify the genetic variability in a natural population of C. prunifera. Thirty seven individuals were sampled in the municipality Macaíba, Rio Grande do Norte, Northeast Brazil. Seventeen ISSR primers were tested, of which 12 amplified DNA and of these, seven were selected to characterize the structure and genetic diversity of the population. The primers that had the highest percentage of polymorphic loci were UBC 841 (16.36%), UBC 842 (15.45%), UBC 857 (12.73%), UBC 859 (10.90%), UBC 840 (10.90%), UBC 813 (10%) and UBC 827 (8.18%), totaling 93 of the 110 loci generated. The polymorphic information content (PIC) of each selected primer ranged from 0.057 (UBC 859) and 0.444 (UBC 841). The optimal number of loci to reliably estimate the genetic diversity for this work was 76 loci, ie, when the correlation reached 0.998 and the stress was less than 0.05. High genetic diversity was found, with the number of observed alleles (na = 2.00), effective alleles (ne = 1.46), Nei's diversity index (He = 0.28) and Shannon index (Ho = 0.44). The value of the coancestry remained within the confidence interval (P > 0.05), indicating no spatial genetic structure, regardless of the distance between plants. ISSR markers were efficient in genetic characterization of individuals' carnauba serving with allowance for management and conservation of the species.

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“…Em análises de diversidade genética de Hancornia speciosa Gomes, foram utilizados seis primers, gerando 63 locos e apenas 30 (47,62%) apresentaram polimorfismo (Costa et al, 2015). Em estudos moleculares com Elaeis guineensis, Chagas et al (2015) também selecionaram seis primers, onde encontraram um total de 68 locos, sendo 34 polimórficos.…”

Section: /6unclassified

Validação do marcador molecular ISSR para detecção de diversidade genética em Plathymenia reticulata Benth.

Souza¹,

Aristil²,

Miranda³

et al. 2018

Agraria

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RESUMO:A espécie Plathymenia reticulata Benth., apresenta grande potencial ecológico sendo utilizada no reflorestamento de áreas degradas, além do alto valor econômico devido sua madeira de ótima qualidade. Uma técnica para fornecer subsídios para programas de conservação e manejo nesta espécie, além da utilização para estudos de diversidade genética é a seleção de marcadores moleculares. Assim, o objetivo deste estudo foi a seleção de primers ISSR (Inter Simple Sequence Repeat) para determinação da estrutura genética, além da quantificação da diversidade genética em uma população natural de P. reticulata, fornecendo dados para estudos de conservação e reflorestamento. A amostragem englobou 15 indivíduos em um fragmento florestal, no município de Guaçuí/ES. Foram selecionados cinco primers, os quais geraram um total de 85 bandas, das quais 56 eram polimórficas. Constatou-se alta diversidade genética fundamentada nos valores do índice de diversidade de Nei (H' = 0,383) e índice de Shannon (I = 0,550). O número de agrupamentos genéticos obtido no dendrograma pelo método UPGMA corroborou com a análise bayesiana revelando dois grupos. Os marcadores ISSR se mostraram eficientes e permitiram identificar a diversidade genética na população amostrada. Palavras-chave: conservação; reflorestamento; variabilidade genética; vinháticoValidation of molecular marker ISSR for detection of genetic diversity in Plathymenia reticulata Benth.ABSTRACT: The species Plathymenia reticulata Benth., has great ecological potential being used for the reforestation of degraded areas, and the high economic value due to its wood of excellent quality. A technique to provide subsidies for conservation and management programs in this species, besides the use for studies of genetic diversity is the selection of molecular markers. Thus, the purpose of this study was the selection of primers ISSR (Inter Simple Sequence Repeat) to determine the genetic structure, as well as quantification of genetic diversity in a natural population of P. reticulata providing data for conservation and reforestation studies. The sample comprised 15 individuals in a forest fragment in the municipality of Guaçuí/ES, Brazil. Five primers were selected, which generated a total of 85 bands, of which 56 were polymorphic. It found high genetic diversity based on the values of Nei's diversity index (H' = 0.383) and Shannon index (I = 0.550). The number of genetic clusters obtained in the dendrogram by UPGMA corroborated with Bayesian's analysis revealed two groups. ISSR were efficient and have identified the genetic diversity in the population sampled.

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Seleção de marcadores ISSR e diversidade genética em uma população de Elaeis guineensis

Cited by 16 publications

References 13 publications

Research Article Anthropization as a determinant factor in the genetic structure of Copernicia prunifera (Arecaceae).

Research Article Anthropization as a determinant factor in the genetic structure of Copernicia prunifera (Arecaceae).

Diversidade genética de Copernicia prunifera com o uso de marcadores moleculares ISSR

Validação do marcador molecular ISSR para detecção de diversidade genética em Plathymenia reticulata Benth.

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