Selected Biological Medicinal Products and Their Veterinary Use

Zygmuntowicz, Aleksandra; Burmańczuk, Artur; Markiewicz, W.

doi:10.3390/ani10122343

Cited by 8 publications

(8 citation statements)

References 41 publications

(51 reference statements)

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“…Despite these concerns, it should be clearly stated that there are both human and equine MSC clinical products produced in FBS-media that have received market approval in Europe, Canada, New Zealand, Japan, and South Korea ( 46 , 47 ). The two veterinary stem cell products currently approved by the European Medicines Agency, HorStem (EquiCord) and Arti-Cell Forte (Boehringer Ingelheim), are both composed of allogeneic equine MSCs grown in FBS.…”

Section: Fbs In Msc Culturementioning

confidence: 99%

A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine

et al. 2022

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Fetal bovine serum (FBS) remains widely used as a supplement in cell culture media used in the isolation and expansion of mesenchymal stromal cells (MSC) despite longstanding practical, clinical, and ethical concerns over its use. As a result, research on alternative culture media supplement solutions that conserve crucial MSC characteristics has become increasingly relevant. Species-specific supplements and serum-free media such as platelet lysate or chemically defined media have been assessed for their effect in MSC cultures regarding proliferation, differentiation, and immunomodulatory capacity. While none of the alternatives offer a complete solution in replacing traditional FBS supplemented media for culturing MSCs for all species, short-term or transitional use of FBS-free media can perform equally well and could address some of the concerns over the use of FBS.

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Section: Fbs In Msc Culturementioning

confidence: 99%

A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine

et al. 2022

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“…The residue was dried under reduced pressure over phosphorous pentoxide. 2 J HH = 13.7), 7.12 (d, 2H, Ar-H, 4 J HH = 2.3), 7.61 (d, 2H, Ar-H, 4 J HH = 2.3), 7.66 (s, 2H, Ar-H), 7.84 (s, 2H, Ar-H). 13 C NMR (CD 3 OD, δ, ppm): 27.82, 27.85, 28.00, 28.08, 28.11, 28.27, 28.37, 28.43, 30.52, 30.65, 30.68, 31.69, 31.78, 31.87, 34.48, 35.15, 35.19, 35.28, 39.97, 40.34, 40.40, 42.…”

Section: Generalmentioning

confidence: 99%

“…In recent years, research aimed at developing DNA vaccines has shown great potential [ 1 ]. These medicinal products are already successfully used in veterinary medicine [ 2 ]. Recently, Inovio Pharmaceuticals Corporation (Plymouth Meeting, PA) proposed a DNA vaccine for the prevention of SARS COVID-19 [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

PAMAM-calix-dendrimers: Synthesis and Thiacalixarene Conformation Effect on DNA Binding

Mostovaya

Padnya

Shiabiev

et al. 2021

IJMS

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A convenient method for the synthesis of the first generation PAMAM dendrimers based on the thiacalix[4]arene has been developed for the first time. Three new PAMAM-calix-dendrimers with the macrocyclic core in cone, partial cone, and 1,3-alternate conformations were obtained with high yields. The interaction of the obtained compounds with salmon sperm DNA resulted in the formation of the associates of the size up to 200 nm, as shown by the UV-Vis spectroscopy, DLS, and TEM. It was demonstrated by the CD method that the structure of the DNA did not undergo significant changes upon binding. The PAMAM-calix-dendrimer based on the macrocycle in cone conformation stabilized DNA and prevented its degradation.

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“…While at-arrival management practices are somewhat dependent upon anticipated risk of BRD development, both inter- and intra-herd level disease prevalence is highly variable [5, 51]. To counter this variability, beef production systems will often administer antimicrobials and/or immunostimulants at arrival to reduce the risk of clinical BRD development and associated production losses [52, 53]. However, immunostimulant administration alone as a metaphylactic protocol for controlling BRD appears to have minimal impact on rates of morbidity [54,55,56].…”

Section: Discussionmentioning

confidence: 99%

“…To counter this variability, beef production systems will often administer antimicrobials and/or immunostimulants at arrival to reduce the risk of clinical BRD development and associated production losses [52,53]. However, immunostimulant administration alone as a metaphylactic protocol for controlling BRD appears to have minimal impact on rates of morbidity [54,55,56].…”

Section: Discussionmentioning

confidence: 99%

Hematological and gene co-expression network analyses of high-risk beef cattle defines immunological mechanisms and biological complexes involved in bovine respiratory disease and weight gain

Scott

Woolums

Swiderski

et al. 2022

Preprint

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Bovine respiratory disease (BRD), the leading disease complex in beef cattle production systems, remains highly elusive regarding diagnostics and disease prediction. Previous research has employed cellular and molecular techniques to describe hematological and gene expression variation that coincides with BRD development. Here, we utilized weighted gene co-expression network analysis (WGCNA) to leverage total gene expression patterns from cattle at arrival and generate hematological and clinical trait associations to describe mechanisms that may predict BRD development. Gene expression counts of previously published RNA-Seq data from 23 cattle (2017; n=11 Healthy, n=12 BRD) were used to construct gene co-expression modules and correlation patterns with complete blood count (CBC) and clinical datasets. Modules were further evaluated for cross-populational preservation of expression with RNA-Seq data from 24 cattle in an independent population (2019; n=12 Healthy, n=12 BRD). Genes within well-preserved modules were subject to functional enrichment analysis for significant Gene Ontology terms and pathways. Genes which possessed high module membership and association with BRD development, regardless of module preservation (“hub genes”), were utilized for protein-protein physical interaction network and clustering analyses. Five well-preserved modules of co-expressed genes were identified. One module (“steelblue”), involved in alpha-beta T-cell complexes and Th2-type immunity, possessed significant correlation with increased erythrocytes, platelets, and BRD development. One module (“purple”), involved in mitochondrial metabolism and rRNA maturation, possessed significant correlation with increased eosinophils, fecal egg count per gram, and weight gain over time. Fifty-two interacting hub genes, stratified into 11 clusters, may possess transient function involved in BRD development not previously described in literature. This study identifies co-expressed genes and coordinated mechanisms associated with BRD, which necessitates further investigation in BRD-prediction research.

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Selected Biological Medicinal Products and Their Veterinary Use

Cited by 8 publications

References 41 publications

A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine

A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine

PAMAM-calix-dendrimers: Synthesis and Thiacalixarene Conformation Effect on DNA Binding

Hematological and gene co-expression network analyses of high-risk beef cattle defines immunological mechanisms and biological complexes involved in bovine respiratory disease and weight gain

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