Selected metabolic biochemical and enzyme activities associated with Besnoitia besnoiti infection in dairy cattle

Alshehabat, Musa A.; Alekish, Myassar O.; Talafha, Abdelsalam Q.

doi:10.1007/s11250-016-1077-7

Cited by 4 publications

(4 citation statements)

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“…The search for serum biomarkers can help with early diagnosis and elucidate the clinical and molecular pathogenesis puzzle of bovine besnoitiosis [ 6 , 22 ]. Previous attempts have focused on haematological and biochemical parameters [ 23 , 24 ]. However, changes in a few biomarkers were observed when animals with well-defined acute, subacute and chronic besnoitiosis were studied compared to another study where only seropositive cattle were compared with seronegative cattle [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previous attempts have focused on haematological and biochemical parameters [ 23 , 24 ]. However, changes in a few biomarkers were observed when animals with well-defined acute, subacute and chronic besnoitiosis were studied compared to another study where only seropositive cattle were compared with seronegative cattle [ 23 , 24 ]. Therefore, a well-characterised panel of clinically affected animals is more convenient in the search for biomarkers, and acute-phase response markers could be of value in bovine besnoitiosis, where a systemic inflammatory reaction is especially intense during the acute phase and focused at the testicular level during acute and chronic besnoitiosis.…”

Section: Discussionmentioning

confidence: 99%

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Changes in serum biomarkers of inflammation in bovine besnoitiosis

et al. 2021

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Background Acute and chronic besnoitiosis in extensive natural-service herds can have relevant effects in the health of bulls and negative consequences in their productive performance. Recent progress has been made in order to elucidate the pathogenesis of this disease. In this context, the study of biomarkers of inflammation in serum would contribute to gaining knowledge about the physiopathology of bovine besnoitiosis. Serological biomarkers could help in early diagnosis and prognosis, as seropositive bulls may have mild or severe testicular lesions. Methods Herein, we have investigated the diagnostic and/or prognostic value of a panel of serum (serological) biomarkers related to inflammation, including total protein, globulin and albumin, haptoglobin (Hp), adenosine deaminase (ADA) paraoxonase-1 (PON-1) and acetylcholinesterase (AChE) in naturally and experimentally B. besnoiti-infected males classified according to different clinical phases of the disease (acute, chronic and subclinical besnoitiosis). Results Results showed a similar response pattern in these biomarkers for naturally and experimentally infected cattle, with a few relevant variations. Most significant changes occurred during the acute phase of infection, although significant changes in a few biomarkers were also observed during the chronic infection. Haptoglobin, albumin, PON-1 and ADA were identified as the biomarkers that showed changes of higher magnitude in the acute phase of the infection, whereas high total protein and globulin values were found in chronically infected cattle. We have described the changes of a panel of inflammatory biomarkers of acute and chronic bovine besnoitiosis. Conclusions In summary, several biomarkers with promising diagnostic value have been identified. The biomarkers associated with acute infection are related to previously reported molecular biomarkers in testicular parenchyma of infected bulls and could help in the diagnosis of early infections and complement results from specific immunoglobulin M (IgM) detection. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Changes in serum biomarkers of inflammation in bovine besnoitiosis

et al. 2021

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“…Plasma samples were stored at −80°C until analysis. The following four metabolic indicators of SK were tested in strict accordance with the kit instructions: i) The plasma GLU concentration (Changchun Huili Biotech Co. Ltd) was measured by means of the glucose oxidase-peroxidase method (Kong et al, 2015); ii) NEFA (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) was measured by the acyl-CoA synthetase/oxidase enzyme colorimetric method (Xia et al, 2009) with a commercially available kit; iii) The plasma BHBA (Bio Vision Incorporated, Milpitas CA) concentration was measured by using the enzyme colorimetric method (Alshehabat et al, 2016); and iv) GH (bovine GH kit, TWp024402j, TWreagent, Shanghai, China) was determined by competitive enzyme-linked immunosorbent assay (Chen et al, 2018). For the GH and GLU biochemical indices, the intra-and inter-assay coefficient of variation (CV%) was <5%.…”

Section: Determination Of Blood Plasma Concentrations Of Metabolic In...mentioning

confidence: 99%

Effects of higher plasma growth hormone levels on subclinical ketosis in postpartum Holstein cows

Mohsin

Zhou

et al. 2022

Annals of Animal Science

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Ketosis is a major metabolic disorder that can lead to huge economic losses in postpartum dairy cows by influencing milk production and reproduction performance. Therefore, it is very important to understand the characteristics and significance of plasma GH levels and dynamic changes in postpartum dairy cows for finding pathogenesis of subclinical ketosis (SK). The present study aimed to determine the role of growth hormone (GH) from the onset of SK to the fifth week postpartum and to explain the variations in GH, and metabolic markers namely, β-hydroxybutyric acid (BHBA), non-esterified fatty acid (NEFA) and glucose (GLU) at early and later SK stages in postpartum Holstein cows. A 5-wk test and an intraday 12-h test were conducted in postpartum Holstein cows. Both tests were carried out every three hours from 10:00–22:00 for 7–14 days postpartum (12-h test: n = 16) to determine plasma concentrations of GH, BHBA, NEFA and GLU. The 5-wk test results showed that GH, BHBA and NEFA concentrations were significantly higher in the SK group during the five-weeks postpartum (p < 0.01); GLU concentration was significantly lower in the SK group (p < 0.01). Intraday 12-h test results revealed that the feeding time affected the plasma concentrations of GH, BHBA, NEFA and GLU. After 1-h of feeding time, GH concentrations decreased, while BHBA, NEFA and GLU concentrations increased. After 4-h of feeding time GH, BHBA and NEFA had the highest plasma concentrations, and GLU the lowest. In both experiments, GH was positively correlated with BHBA, NEFA, and negatively correlated with GLU. It can be suggested that GH has a potential role in development and aetiology of subclinical ketosis.

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“…These parasites have been reported to cause alopecia, hyperkeratosis and tissue cysts in mucous membranes of the host animals (Gutiérrez-Expósito et al, 2017). Bovine besnoitiosis was recognized as an extremely important disease in several countries in Asia, Africa, Middle East, South America and Europe due to the major economic losses in the cattle industry in terms of low milk production, poor health, male sterility and death (Gazzonis et al, 2014;Waap et al, 2014;García-Lunar et al, 2015;Gollnick et al, 2015;Alshehabat et al, 2016;Verma et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Besnoitiosis: An Emerging Parasitic Disease in Yaks (Bos grunniens) and Tibetan Sheep (Ovies aries) on the Qinghai Tibetan Plateau, China

Li¹

2019

PVJ

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Besnoitiosis is an emerging disease in Tibetan sheep and yaks in Qinghai Tibetan plateau (QTP) of China. That is why a survey was conducted for the seroprevalence and detection of associated risk factors of this disease in Tibetan sheep and yaks in QTP of China. A total of 1092 and 1449 serum samples were collected from Tibetan sheep and yaks respectively and checked by employing commercial ELISA kits. The seroprevalence of besnoitiosis in Tibetan sheep was found to be 3.21% ranging from 0 to 5.6% in different counties. The male and female sheep were found positive with 3.15 and 3.25% cases respectively for this disease. A total of 16/1449 (1.10%) yaks were found to be positive for besnoitiosis ranging from 0-2.03% from different counties. In years of 2011-17, the seroprevalence has been ranged from 0-4.10%. Based on conditional stepwise logistic regression analysis, it can be speculated that the varying climatic changes in QTP region and age are the influencing risk factors in causing besnoitiosis in Tibetan sheep and yaks. The survey herein has highlighted the risk and rising potential for this parasitic disease in yaks and Tibetan sheep on the remote plateau. ©2019 PVJ. All rights reserved Key words: Apicomplexan parasite Besnoitia ELISA Tibet To Cite This Article: Li K, Zhang L, Luo H, Mehmood K, Shahzad M and Naseer MU, 2019. Besnoitiosis: an emerging parasitic disease in yaks (bos grunniens) and tibetan sheep (ovies aries) on the qinghai tibetan plateau, China. Pak Vet J, 39(3): 447-450. http://dx.

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Selected metabolic biochemical and enzyme activities associated with Besnoitia besnoiti infection in dairy cattle

Cited by 4 publications

References 8 publications

Changes in serum biomarkers of inflammation in bovine besnoitiosis

Changes in serum biomarkers of inflammation in bovine besnoitiosis

Effects of higher plasma growth hormone levels on subclinical ketosis in postpartum Holstein cows

Besnoitiosis: An Emerging Parasitic Disease in Yaks (Bos grunniens) and Tibetan Sheep (Ovies aries) on the Qinghai Tibetan Plateau, China

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