Brassinosteroids (BRs) act as immune-modulators for generating plant growth and development. It regulated either activation or suppression of various key enzymatic reactions, activation of synthesis of protein, and generation of various defense modulating compounds for the plant kingdom. BRs play a vital role in regulating cellular differentiation, pollen development, fruit ripening, and quality seed formation. BRs regulates the various physiological process including root growth during nutrient deficiency such as nitrogen, phosphorus, boron and tends to signal the nutrient distribution in the rhizosphere level for better growth and high yield in crop plants. This review highlighted the role of BRs in plant growth and development and stress response, understanding the BR pathway, the molecular mechanism of BR signaling in various tissues, crosstalk between BRs and other phytohormones, gene involves in the brassinosteroids signaling pathway, biosynthesis and role of BRs in biomass production and crop yield.