2018
DOI: 10.1139/gen-2017-0176
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Selecting and validating reference genes for quantitative real-time PCR in Plutella xylostella (L.)

Abstract: Gene expression analysis provides important clues regarding gene functions, and quantitative real-time PCR (qRT-PCR) is a widely-used method in gene expression studies. Reference genes are essential for normalizing and accurately assessing gene expression. In the present study, 16 candidate reference genes (ACTB, CyPA,

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Cited by 11 publications
(7 citation statements)
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“…ACTB is widely used as a reference gene in qPCR analysis [2325]. However, in this study, the expression of ACTB was significantly down-regulated with a 0.63-fold change in Hcy-treated HUVECs (Fig 2B).…”
Section: Resultsmentioning
confidence: 61%
“…ACTB is widely used as a reference gene in qPCR analysis [2325]. However, in this study, the expression of ACTB was significantly down-regulated with a 0.63-fold change in Hcy-treated HUVECs (Fig 2B).…”
Section: Resultsmentioning
confidence: 61%
“…Therefore, a stable endogenous reference gene is essential for relative quantification in gene expression analyses. Currently, many reference genes have been recommended in different insect species, including sweet potato whitefly Bemisia tabaci (Gennadius) [ 32 34 ], brown planthopper Nilaparvata lugens (Stål) [ 35 , 36 ], diamondback moth Plutella xylostella (Linnaeus) [ 15 , 17 ], fruit fly Drosophila melanogaster (Meigen) [ 37 ], pea aphid Acyrthosiphon pisum (Harris) [ 38 ] and peach aphid M . persicae (Sülzer) [ 19 ].…”
Section: Discussionmentioning
confidence: 99%
“…The use of unsuitable reference genes in RT-qPCR has been shown to lead to false results and then to erroneous interpretations [ 10 14 ]. Choosing the most stable reference gene in a specific tissue and treatment is significantly important to precisely clarify the expression level of the target gene [ 14 , 15 ]. To date, more than one reference gene is required for accurate normalization in RT-qPCR analysis [ 8 , 9 ].…”
Section: Introductionmentioning
confidence: 99%
“…P. xylostella ribosomal protein S4 (rpS4, XM_011555372) was amplified and used as internal reference for the quantitative PCR analysis of different insect growth stages. Ribosomal protein L8 (rpL8, NM_001305488) was amplified and used as internal reference for quantitative PCR analyses of different tissues [38,39]. Elongation factor 1 (ef1, EF417849) was also amplified and used to verify the quantitative results (primers shown in Table 1).…”
Section: Quantitative Real-time Pcrmentioning
confidence: 99%