Selecting Lactic Acid Bacteria for Their Safety and Functionality by Use of a Mouse Colitis Model

Daniel, Catherine; Poiret, Sabine; Goudercourt, Denise; Dennin, Véronique; Leyer, Gregory; Pot, Bruno

doi:10.1128/aem.00109-06

Cited by 118 publications

(81 citation statements)

References 48 publications

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“…Based on the same mechanism as lactulose, pH modulation was observed with all tested L. plantarum strains into the colon, but not into the ileum, probably due the poor survival of L. plantarum during the passage through the stomach and the duodenum. 40 Experiments performed in our laboratory where treatment was administered orally, intrarectally, or by both routes showed that only the last two conditions produced a beneficial effect on blood ammonia levels (data not shown), suggesting that in loco colonic administration is sufficient to reduce ammonia. Enumeration of Lactobacillus confirmed a very low number of CFU (Ͻ10 3 CFU/mL) inside the stomach and the small intestine after oral administration, maybe due to strong acidic conditions and presence of pepsin and lysozyme.…”

Section: Discussionmentioning

confidence: 99%

Control of acute, chronic, and constitutive hyperammonemia by wild‐type and genetically engineered Lactobacillus plantarum in rodents†

et al. 2008

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Hyperammonemia is a common complication of acute and chronic liver diseases. Often accompanied with side effects, therapeutic interventions such as antibiotics or lactulose are generally targeted to decrease the intestinal production and absorption of ammonia. In this study, we aimed to modulate hyperammonemia in three rodent models by administration of wild-type Lactobacillus plantarum, a genetically engineered ammonia hyperconsuming strain, and a strain deficient for the ammonia transporter. Wild-type and metabolically engineered L. plantarum strains were administered in ornithine transcarbamoylase-deficient Sparse-fur mice, a model of constitutive hyperammonemia, in a carbon tetrachloride rat model of chronic liver insufficiency and in a thioacetamide-induced acute liver failure mice model. Constitutive hyperammonemia in Sparse-fur mice and hyperammonemia in a rat model of chronic hepatic insufficiency were efficiently decreased by Lactobacillus administration. In a murine thioacetamide-induced model of acute liver failure, administration of probiotics significantly increased survival and decreased blood and fecal ammonia. The ammonia hyperconsuming strain exhibited a beneficial effect at a lower dose than its wild-type counterpart. Improved survival in the acute liver failure mice model was associated with lower blood ammonia levels but also with a decrease of astrocyte swelling in the brain cortex. Modulation of ammonia was abolished after administration of the strain deficient in the ammonium transporter. Intestinal pH was clearly lowered for all strains and no changes in gut flora were observed. Conclusion: Hyperammonemia in constitutive model or after acute or chronic induced liver failure can be controlled by the administration of L. plantarum with a significant effect on survival. The mechanism involved in this ammonia decrease implicates direct ammonia consumption in the gut. (HEPATOLOGY 2008;48:1184-1192 H yperammonemia (HA) is a well-known complication of acute and chronic liver diseases and plays a central role in the pathogenesis of hepatic encephalopathy (HE). [1][2][3][4][5] This neurological dysfunction results, at least in part, from an increase in plasma ammonia level and the severity of the symptoms correlates with blood ammonia level. 6-9 Animal models used in studying hyperammonemic disorders are multiple: fulminant hepatic failure, 10 chronic liver failure, 11 or urea cycle deficiency. 12 Ammonia is produced by many tissues but its major external source results from deaminase and urease activities of the gut flora. Although its absorption occurs through the intestinal epithelium, ammonia is carried by the portal vein into the liver where it is metabolized into urea. 13 Classical treatments of HE, except through liver transplantation and liver replacement therapies, consist in decreasing the ammonia production of urease-positive bacteria by antibiotics or decreasing the ammonia absorption into the gut by acidifying the colon content with nonabsorbable sugars such as lactulose. 4,[13][14][1...

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Section: Discussionmentioning

confidence: 99%

Control of acute, chronic, and constitutive hyperammonemia by wild‐type and genetically engineered Lactobacillus plantarum in rodents†

et al. 2008

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“…qPCRs were conducted as previously described [10,11] . For characterization of the fecal microbiota PCR primers were designed targeting B. lactis according to Gueimonde and co-workers [17] .…”

Section: Analysis Of Microbiota By Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

“…Thus we chose to study the effects of selected probiotic bacteria in allergic rhinitis in children during the birch pollen season. The specific strains used in this study were selected based on their anti-inflammatory properties and an expected effect in promoting a Th1 type of immune response [11,12] . The study population was chosen to cover subjects with confirmed birch pollen allergy.…”

Section: Introductionmentioning

confidence: 99%

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Specific probiotics alleviate allergic rhinitis during the birch pollen season

Ouwehand¹,

Nermes²,

Collado³

et al. 2009

WJG

114

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AIM:To investigate whether birch pollen allergy symptoms are linked with gut microbiota changes and whether probiotics have an effect on these. METHODS:Forty seven children with confirmed birch pollen allergy were randomized to receive either a probiotic combination of Lactobacillus acidophilus (L . a c i d o p h i l u s ) N C F M T M ( ATC C 7 0 0 3 9 6 ) a n dBifidobacterium lactis (B. lactis ) Bl-04 (ATCC SD5219) or placebo in a double-blind manner for 4 mo, starting prior to onset of the birch pollen season. Symptoms were recorded in a diary. Blood samples were taken for analysis of cytokines and eosinophils. Fecal samples were analysed for microbiota components, calprotectin and IgA. Nasal swabs were taken for analysis of eosinophils. RESULTS:The pollen season induced a reduction in Bifidobacterium , Clostridium and Bacteroides which could not be prevented by the probiotic intervention. During the intervention, significantly higher numbers of B. lactis 11.2 × 10 7 ± 4.2 × 10 7 vs 0.1 × 10 7 ± 0.1 × 10 7 bacteria/g feces (P < 0.0001) and L. acidophilus NCFM TM 3.5 × 10 6 ± 1.3 × 10 6 vs 0.2 × 10 6 ± 0.1 × 10 6 bacteria/g feces (P < 0.0001) were observed in the probiotic group compared to the placebo group. During May, there was a tendency for fewer subjects, (76.2% vs 95.2%, P = 0.078) to report runny nose, while during June, fewer subjects, 11.1% vs 33.3%, reported nasal blocking in the probiotics group (P = 0.101). Concomitantly, fewer subjects in the probiotic group had infiltration of eosinophils in the nasal mucosa compared to the placebo group, 57.1% vs 95% (P = 0.013). Eye symptoms tended to be slightly more frequent in the probiotic group, 12.5 d[interquartile range (IQR) 6-18] vs 7.5 d (IQR 0-11.5) (P = 0.066) during May. Fecal IgA was increased in the placebo group during the pollen season; this increase was prevented by the probiotics (P = 0.028). CONCLUSION:Birch pollen allergy was shown to be associated with changes in fecal microbiota composition. The specific combination of probiotics used was shown to prevent the pollen-induced infiltration of eosinophils into the nasal mucosa, and indicated a trend for reduced nasal symptoms.

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“…[1][2][3][4][5][6] LAB have a positive stimulus on ingestion and the immune system. [7] LAB are reported for non-specific activation of the host's defense system.…”

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confidence: 99%

Screening of lactic acid bacteria with immune modulating property, and the production of lactic acid bacteria mediated fermented soymilk

Sirilun¹,

Chaiyasut²,

Kesika³

et al. 2017

Natl J Physiol Pharm Pharmacol

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Background: Lactic acid bacteria (LAB) are reported to have immune activating property. The genetically modified LAB strains producing interleukin (IL) -10 were under consideration for the treatment of inflammatory bowel diseases (IBD). Soybean is rich in necessary nutrients, and the fermented soybean products are known for its health benefits. Aims and Objectives: The current study was aimed to screen LAB with the immune activating property, and production and evaluation of LAB mediated fermented soymilk product. Materials and Methods: Screening of LAB from the fermented foods collected in Chiang Mai. To select a potent LAB strain, the LAB isolates were scrutinized for acid tolerant, bile tolerant, growth rate, antimicrobial ability, adhesion property, and induction of IL-10 production. The physical properties of fermented soymilk (FSM) were evaluated, and in vitro activation of IL-10 by FSM was assessed using Caco-2 cell line. Results: About 742 LAB strains were isolated from 335 fermented food samples. Based on the tested properties, LAB33 was selected as a potent LAB strain, and used for the fermentation of soymilk for 30 days. The LAB33 FSM was superior in quality regarding probiotic nature and microbiological safety. Conclusion: The results of the study suggested that LAB33 is the potent probiotic strain for the production of soymilk-based fermented functional food supplements for the betterment of IBD patients. Further, strain identification is required to register the strain in a public repository, and sustainable utilization for the benefit of mankind.

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Selecting Lactic Acid Bacteria for Their Safety and Functionality by Use of a Mouse Colitis Model

Cited by 118 publications

References 48 publications

Control of acute, chronic, and constitutive hyperammonemia by wild‐type and genetically engineered Lactobacillus plantarum in rodents†

Control of acute, chronic, and constitutive hyperammonemia by wild‐type and genetically engineered Lactobacillus plantarum in rodents†

Specific probiotics alleviate allergic rhinitis during the birch pollen season

Screening of lactic acid bacteria with immune modulating property, and the production of lactic acid bacteria mediated fermented soymilk

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