Selecting the most competent sperm for assisted reproductive technologies

Jeyendran, Rajasingam S.; Caroppo, Ettore; Rouen, Alexandre; Anderson, Andrew; Puscheck, Elizabeth E.

doi:10.1016/j.fertnstert.2019.03.024

Cited by 39 publications

(31 citation statements)

References 117 publications

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“…Samples with higher volumes were separated in different gradients. The resultant pellet, containing the spermatozoa fraction with higher percentage of motility and viability [ 22 , 23 ], was collected and washed 2 times in sterile PBS followed by a centrifugation of 500× g for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

et al. 2020

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Spermatozoa capacitation is a complex process that requires specific ionic and energetic conditions to support biochemical alterations leading to motility hyperactivation. However, human sperm capacitation is still poorly understood. Herein, we studied the effects of glucose on human sperm capacitation. Healthy men seminal samples (n = 55) were submitted to a density gradient centrifugation and incubated in capacitating conditions in the absence or presence of increasing glucose concentrations (0, 5.5, 11, and 22 mM). Viability and total motility were accessed. Phosphotyrosine levels were measured. Mitochondrial activity and endogenous ROS production were evaluated. Oxidative stress-induced damage was analyzed. Culture media was collected and analyzed by 1H-NMR. Our results show that glucose is essential for human sperm capacitation and motility. Notably, we observed that mitochondrial activity increased even in the absence of glucose. This increased mitochondrial activity was followed by a ROS overproduction, although no oxidative stress-induced damage was detected. Our results show that glucose is essential for capacitation but mitochondrial activation is independent from its stimuli. ROS overproduction may take part on a finely regulated signaling pathway that modulates or even activates capacitation. Taken together, our results constitute a paradigm shift on human sperm capacitation physiology.

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Section: Methodsmentioning

confidence: 99%

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

et al. 2020

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“…Spermatozoa are complex cells influenced by a myriad of internal and external stimuli (such as pH, ionic concentration or temperature), with several factors that may compromise its fertilising potential still remaining elusive to identify. In fact, new devices for diagnosis are developed based on the novel data produced by the scientific community, such as computer-assisted semen analysis (CASA) systems, analysis of the DNA damage, accessing sperm telomere length or the adoption of stricter criteria for sperm morphology evaluation (Jeyendran, Caroppo, Rouen, Anderson, & Puscheck, 2019;Lopes, Oliveira, & Sousa, 2019). In particular, assays for analysing sperm DNA integrity have been extensively investigated over the past de-cade (Majzoub, Arafa, Elbardisi, & Agarwal, 2020;Panner Selvam, Sengupta, & Agarwal, 2020).…”

Section: S Tandard S Emen Analys Ismentioning

confidence: 99%

“…In hypoosmotic conditions, the tail of sperm cells with a normal functional membrane swell or curl due to water influx, which could be used as an indicative of sperm potential (Jeyendran, Van der Ven, Perez-Pelaez, Crabo, & Zaneveld, 1984). The HOST has already been established as a diagnostic test and is currently used as a reliable indicator of the sperm quality in clinical practice (Jeyendran et al, 2019). However, the possibility that the HOST could be applied as a sperm selection method for the ICSI procedure was first introduced in 1994.…”

Section: Hypo-osmotic Swelling Testmentioning

confidence: 99%

Sperm selection strategies and their impact on assisted reproductive technology outcomes

et al. 2020

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Assisted reproductive technologies (ART) revolutionised human fertility by providing treatment to patients previously considered incapable for reproductive purposes (Henkel & Schill, 2003). In 2017, it was estimated that approximately 6 million infants were born through ART since 1978, when the first baby produced by in vitro fertilisation (IVF) was born. It has been reported that about half of these births were in the past decade, highlighting the increasing use of these technologies, (Hunter, 2017). Initially, ART research was mainly focused on comprehending the factors that increase the likelihood of a successful pregnancy and

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“…Sperm selection is a key step in ART that influences both the treatment success rate and offspring health. 74 Standard methods of sperm selection in ART treatment include density gradient centrifugation or swim-up. Both techniques isolate a population of highly motile, morphologically normal sperm and result in similar ART outcomes.…”

Section: New Developments In Sperm Selection In Artmentioning

confidence: 99%

An update on the management of male infertility

Karavolos

Panagiotopoulou²,

AlAhwany

et al. 2020

The Obstetric & Gynaecologis

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Male infertility underlies or contributes to up to 50% of infertility cases; current therapeutic interventions rely on assisted reproductive technology (ART), as medical or surgical treatments have limited value in enhancing semen quality or parameters. Lifestyle factors that affect male fertility could offer a therapeutic opportunity; however, their modification seems to be of variable benefit. In the quest for sperm functional assessment and selection tests, there is controversy over which patients, if any, should be tested for sperm DNA fragmentation, as well as which test to perform. Sperm selection techniques for intracytoplasmic sperm injection do not appear to significantly improve treatment outcomes or live birth rates. Routinely performed genetic tests are effective in determining aetiology in approximately 20% of infertile men; however, newer genetic tests could enhance diagnosis and change the future management of male infertility. Learning objectives To summarise the key lifestyle factors that affect male fertility. To appraise the currently available investigations for sperm testing and selection. To describe the genetic tests currently available to identify the aetiology of male infertility, including emerging technologies in the field of genetics and personalised genomics. Ethical issues How to deal with couples' requests for unproven medical interventions to manage male infertility?

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Selecting the most competent sperm for assisted reproductive technologies

Cited by 39 publications

References 117 publications

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

Sperm selection strategies and their impact on assisted reproductive technology outcomes

An update on the management of male infertility

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