Selection and characterization of mutant cotton (Gossypium hirsutum L.) cell lines resistant to sulfonylurea and imidazolinone herbicides

Rajasekaran, K.; Grula, John W.; Anderson, David M.

doi:10.1016/0168-9452(96)04444-5

Cited by 56 publications

(41 citation statements)

References 26 publications

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“…Selection experiments using cell cultures in the absence of mutator chemicals have observed resistance cell lines at frequencies of 10 −7 in cotton (Rajasekaran et al, 1996) and 5 × 10 −8 in tobacco (Harms and DiMaio, 1991). As cell cultures are grown over a limited number of generations, the frequencies measured are likely to be closer to the mutation frequency than to the equilibrium frequency of resistant alleles.…”

Section: Discussionmentioning

confidence: 99%

Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate synthase-inhibiting herbicides in Lolium rigidum

2002

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The frequency of individuals resistant to two acetolactate synthase (ALS)-inhibiting herbicides in three previously untreated populations of Lolium rigidum was determined. The frequency of individuals resistant to the sulfonylurea herbicide sulfometuron-methyl varied from 2.2 × 10 −5 to 1.2 × 10 −4 and the frequency of individuals resistant to the imidazolinone herbicide imazapyr varied from 1 × 10 −5 to 5.8 × 10 −5 depending on the population. Application of sulfometu-

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Section: Discussionmentioning

confidence: 99%

Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate synthase-inhibiting herbicides in Lolium rigidum

2002

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“…The plant ALS regulatory (small) subunit has been shown to enhance the catalytic activity of the large subunit and to confer sensitivity to feedback inhibition by branchedchain amino acids (Lee & Duggleby 2001). Plants and cultured plant cells resistant to SUand IM-type ALS-inhibiting herbicides have been generated using conventional mutation breeding methods and in vitro cell selection (Hart et al, 1993;Newhouse et al, 1991;Rajasekaran et al, 1996). ALS genes encoding catalytic subunits have been cloned from some Fig.…”

Section: Als and Als-inhibiting Herbicidesmentioning

confidence: 99%

Application of Mutated Acetolactate Synthase Genes for Herbicide Resistance to Crop Improvement

Shimizu¹,

Kawai²,

Kaku³

et al. 2011

Herbicides, Theory and Applications

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“…Amino acid substitutions at Ala122 and Ser653 confer high levels of resistance to imidazolinone herbicides (IMIs), whereas substitutions at Pro197 endow high levels of resistance against sulfonylureas and provide low-level resistance against IMIs and triazolopyrimidine herbicides (18)(19)(20)(21)(22). Substitutions at Trp574 endow high levels of resistance to imidazolinones, sulfonylureas, and triazolopyrimidines (19,(23)(24)(25)(26), whereas substitutions at Ala205 provide resistance against all AHAS-inhibiting herbicides (24,27).…”

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confidence: 99%

Single nucleotide mutation in the barley acetohydroxy acid synthase ( AHAS ) gene confers resistance to imidazolinone herbicides

Lee¹,

Rustgi²,

Kumar³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Induced mutagenesis can be an effective way to increase variability in self-pollinated crops for a wide variety of agronomically important traits. Crop resistance to a given herbicide can be of practical value to control weeds with efficient chemical use. In some crops (for example, wheat, maize, and canola), resistance to imidazolinone herbicides (IMIs) has been introduced through mutation breeding and is extensively used commercially. However, this production system imposes plant-back restrictions on rotational crops because of herbicide residuals in the soil. In the case of barley, a preferred rotational crop after wheat, a period of 9–18 mo is required. Thus, introduction of barley varieties showing resistance to IMIs will provide greater flexibility as a rotational crop. The objective of the research reported was to identify resistance in barley for IMIs through induced mutagenesis. To achieve this objective, a sodium azide-treated M 2 /M 3 population of barley cultivar Bob was screened for resistance against acetohydroxy acid synthase (AHAS)-inhibiting herbicides. The phenotypic screening allowed identification of a mutant line showing resistance against IMIs. Molecular analysis identified a single-point mutation leading to a serine 653 to asparagine amino acid substitution in the herbicide-binding site of the barley AHAS gene. The transcription pattern of the AHAS gene in the mutant (Ser653Asn) and WT has been analyzed, and greater than fourfold difference in transcript abundance was observed. Phenotypic characteristics of the mutant line are promising and provide the base for the release of IMI-resistant barley cultivar(s).

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Selection and characterization of mutant cotton (Gossypium hirsutum L.) cell lines resistant to sulfonylurea and imidazolinone herbicides

Cited by 56 publications

References 26 publications

Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate synthase-inhibiting herbicides in Lolium rigidum

Evolution of herbicide resistance in weeds: initial frequency of target site-based resistance to acetolactate synthase-inhibiting herbicides in Lolium rigidum

Application of Mutated Acetolactate Synthase Genes for Herbicide Resistance to Crop Improvement

Single nucleotide mutation in the barley acetohydroxy acid synthase ( AHAS ) gene confers resistance to imidazolinone herbicides

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