Selection and characterization of novel DNA aptamers specifically recognized by Singapore grouper iridovirus-infected fish cells

Li, Pengfei; Wei, Shina; Zhou, Lingli; Yang, Min; Yu, Yang; Wei, Jingguang; Jiang, Guangde; Qin, Qiwei

doi:10.1099/jgv.0.000270

Cited by 38 publications

(65 citation statements)

References 37 publications

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“…As the molecular characteristics of cells infected by virus are critical in understanding viral pathogenesis (Gerold & Pietschmann, ), cell lines isolated from fishes could also be applied in studying the mechanisms of viral infection. In this study, the previously selected aptamer (Q2) (Li et al ., 2015 a , b ), which was proved to specifically bound to SGIV‐infected GS cells, could also recognize different types of SGIV‐infected TOHK cells; therefore, it is suggested that SGIV could invade different cells through similar pathways, or that it causes similar modifications in the membranes of infected cells. More work should be done to identify the targets of aptamers in both SGIV‐infected TOHK cells and SGIV‐infected GS cells.…”

Section: Discussionmentioning

confidence: 99%

“…All experimental procedures on fish were carried out in accordance with the guidelines and were approved by the ethical committee of South China Sea Institute of Oceanology, Chinese Academy of Sciences, as described previously (Li et al ., 2015 a , b ). All sections of this report adheres to the ARRIVE guidelines for reporting animal research (http://www.nc3rs.org.uk).…”

Section: Methodsmentioning

confidence: 99%

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Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

Zhou

Wei

et al. 2017

Journal of Fish Biology

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A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28° C and the optimum foetal bovine serum concentration in L-15 medium was 10%. The TOHK cells have a diploid chromosome number of 2N = 54. The transfection efficiency of TOHK cells was 7·5% at the 15th passage and 72% at the 40th passage. The transfection efficiency in TOHK cells was high, so these cells are suitable for foreign gene expression. The cytotoxic effects of heavy metals and extracellular products from Vibrio anguillarum and Vibrio alginolyticus were demonstrated in TOHK cells, so this TOHK cell line could also be applied in environmental monitoring of heavy metals and pathogenic bacteria. TOHK cell line showed high virus susceptibility, such as grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). Then, TOHK cell line could be used for the study of viral pathogenesis and the development of antiviral strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

Zhou

Wei

et al. 2017

Journal of Fish Biology

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“…To date, the application of aptamers technology in infectious disease diagnosis of aquatic animal is mostly limited to viral infections. Several aptamers have been reported for viral detections (Aoki & Hirono, ; Li et al., ; Li, Zhou, et al., , Zhou et al., , ) and the detection of virus‐infected cells (Li, Wei, et al., ; Li et al., , ), and aptamers that manifesting antiviral property (Hwang et al., ; Li et al., ; Li, Zhou, et al., ; Zhou et al., ).…”

Section: Treatment and Preventionmentioning

confidence: 99%

Molecular biology of Macrobrachium rosenbergii nodavirus infection in giant freshwater prawn

Low

Yusoff²,

Kuppusamy³

et al. 2018

Journal of Fish Diseases

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Macrobrachium rosenbergii nodavirus (MrNV) has been threatening the giant freshwater prawn aquaculture since 1997, causing white tail disease in the prawn species that leads to 100% lethality of the infected postlarvae. Comprehension of the viral infectivity and pathogenesis at molecular biology level has recently resolved the viral capsid protein and evidenced the significant difference in the viral structural protein compared to other nodaviruses that infect fish and insect. Cumulative researches have remarked the proposal to assert MrNV as a member of new genus, gammanodavirus to the Nodaviridae family. The significance of molecular biology in MrNV infection is being highlighted in this current review, revolving the viral life cycle from virus binding and entry into host, virus replication in host cell, to virus assembly and release. The current review also highlights the emerging aptamers technology that is also known as synthetic antibody, its application in disease diagnosis, and its prophylactic and therapeutic properties. The future perspective of synthetic virology technology in understanding viral pathogenesis, as well as its potential in viral vaccine development, is also discussed.

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“…At present, aptamers have made great progress in the ichthyopathology field. The aptamers against some severe aquatic viruses, such as Singapore grouper iridovirus (Li et al, ; Li, Wei, et al, ; Li, Zhou, et al, ), red‐spotted grouper nervous necrosis virus (Zhou et al, ), Trachinotus ovatus NNV (Yu, Liu, Wei, et al, ), spring viraemia of carp virus (Yu et al, ), Hirame rhabdovirus virus (Hwang et al, ), viral haemorrhagic septicaemia virus (Punnarak et al, ) and soft‐shelled turtle iridovirus (Li, Wei, et al, ; Li, Zhou, et al, ), have been successfully generated. There are also kinds of aptamers generated against various bacterial targets, such as Lactobacillus acidophilus , V. parahaemolyticus and Salmonella typhimurium (Duan, Wu, Chen, Huang, & Wang, ; Duan et al, ), V. alginolyticus (Yu, Liu, Su, et al, ; Tang, Zheng, Yan, Li, & Li, ; Zheng et al, ), V. harveyi (Zheng, Hao, Song, & Liu, ) and Aeromonas hydrophila (Li, Wang, Chen, & Li, ).…”

Section: Discussionmentioning

confidence: 99%

“…There are also kinds of aptamers generated against various bacterial targets, such as Lactobacillus acidophilus , V. parahaemolyticus and Salmonella typhimurium (Duan, Wu, Chen, Huang, & Wang, ; Duan et al, ), V. alginolyticus (Yu, Liu, Su, et al, ; Tang, Zheng, Yan, Li, & Li, ; Zheng et al, ), V. harveyi (Zheng, Hao, Song, & Liu, ) and Aeromonas hydrophila (Li, Wang, Chen, & Li, ). Aptamers have been widely applied in different important research areas, including drug‐specific delivery (Loo et al, ; Zhao et al, ), pathogen diagnosis (Li et al, ; Li, Wei, et al, ; Li, Zhou, et al, ; Teng et al, ; Yu, Liu, Wei, et al, ; Zhou et al, ), toxin analysis (Huang et al, ; Yan et al, ) and disease pathogenesis research (Bunka & Stockley, ). The excellent characteristics of easy synthesis, low immunogenicity, innocuity and high specificity and stability make aptamers ideal alternatives to antibodies (Toh et al, ).…”

Section: Discussionmentioning

confidence: 99%

Development of novel aptamer‐based enzyme‐linked apta‐sorbent assay (ELASA) for rapid detection of mariculture pathogen Vibrio alginolyticus

Liu

Xiao

et al. 2019

Journal of Fish Diseases

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As the major opportunistic pathogen to both marine animals and humans, Vibrio alginolyticus (V. alginolyticus) has caused heavy economic losses to mariculture. ssDNA aptamer VA2 targeting live V. alginolyticus was generated by systematic evolution of ligands by exponential enrichment (SELEX) technology in our previous study. In this study, we first developed aptamer (VA2)‐based enzyme‐linked apta‐sorbent assay (VA2‐ELASA) for rapid detection of mariculture pathogen V. alginolyticus. The VA2‐ELASA could achieve the rapid detection for V. alginolyticus infection with high specificity and sensitivity. The VA2‐ELASA could specifically identify V. alginolyticus, but not other non‐target bacterial strains. VA2‐ELASA could detect V. alginolyticus at the concentration of 5 × 104/ml, the incubation time short to 1 min and the incubation temperature as high as 45°C, which proved sensitivity and stability of the novel VA2‐ELASA in this study. It took less than one hour to accomplish the detection process by VA2‐ELASA. The characteristics of specificity, sensitivity and easy operation make VA2‐ELASA a novel useful technology for the rapid diagnosis of pathogen V. alginolyticus in mariculture.

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Selection and characterization of novel DNA aptamers specifically recognized by Singapore grouper iridovirus-infected fish cells

Cited by 38 publications

References 37 publications

Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

Molecular biology of Macrobrachium rosenbergii nodavirus infection in giant freshwater prawn

Development of novel aptamer‐based enzyme‐linked apta‐sorbent assay (ELASA) for rapid detection of mariculture pathogen Vibrio alginolyticus

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