Selection and identification of oleaginous yeast isolated from soil, animal feed and ruminal fluid for use as feed supplement in dairy cattle

Paserakung, A.; Pattarajinda, V.; Vichitphan, Kanit; Froetschel, M. A.

doi:10.1111/lam.12475

Cited by 17 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another molasses utilizing novel isolate from soil Trichosporon asahii have also been reported as oleaginous, which is new addition in already reported oleaginous genera of yeasts Trichosporon (Paserakung et al . ). There are several other recognized oleaginous species showing lipid accumulation on synthetic carbon substrates, especially glucose, some of which have been listed in Table .…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Comprehending the influence of critical cultivation parameters on the oleaginous behaviour of potent rotten fruit yeast isolates

Diwan

Gupta

2018

J Appl Microbiol

View full text Add to dashboard Cite

Two potent oleaginous yeast isolates were studied, and this is the only successive report describing oleaginous behaviour of P. kudriavzevii. The cultivation parameters such as C source, concentration and C/N ratio which critically influence the oleaginous behaviour, lipid content, yield and composition have been accessed so as to provide comprehensive understanding on single cell oil production from these isolates. Study progressively contributes to current and upcoming researches in microbial oils and the characteristic fatty acid, and TAG profile provides important leads for their putative applicabilities.

show abstract

Section: Discussionmentioning

confidence: 97%

“…), organic wastes (Kitcha and Cheirsilp ; Paserakung et al . ) and even from rotten fruits, and the only study reporting P. kudriavzevii as oleaginous species also isolated this yeast from rotten fruits (Sankh et al . ).…”

Section: Discussionmentioning

confidence: 99%

Comprehending the influence of critical cultivation parameters on the oleaginous behaviour of potent rotten fruit yeast isolates

Diwan

Gupta

2018

J Appl Microbiol

View full text Add to dashboard Cite

show abstract

“…To increase the biomass of yeast, a substrate such as soluble carbohydrate and nitrogen must provide sufficient supplies for the growth of yeast cells [27]. Paserakung et al [6] reported that increasing molasses concentration from 8% to 16% resulted in the greatest biomass production of 25.9% obtained from Trichosporon asahii. In addition, Johnson et al [13], who studied the effects of different single-substrate carbon sources such as molasses, glucose, and sucrose with limited nitrogen sources in media solutions on the biomass production of Rhodotorula glutinis IIP-30, found that biomass production increased by 87.8%…”

Section: The Biomass Production and Cellulase Enzyme Activity Of Isolmentioning

confidence: 99%

“…Sirisan et al [5] found that isolated Kodamaea ohmeri, yeast from rumen fluids can convert lactic acid to energy, which affects optimum ruminal pH and decreases acidosis in dairy cattle. Moreover, Paserakung et al [6] revealed that Tricosporon asahii GSY10, a yeast isolated from the environment, was a potential alternative fat source in ruminant diets. A recent study by Intanoo et al [7] reported that isolated Kluyveromyces marxianus yeast from rumen fluids showed potential as an aflatoxin-detoxifying agent in dairy cattle.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Novel Yeasts from Rumen Fluids for Potential Use as Additives for Ruminant Feeding

Suntara¹,

Cherdthong²,

Wanapat³

et al. 2020

Preprint

View full text Add to dashboard Cite

We hypothesized that rumen fluid with yeast producing cellulase enzyme can occur and also produces a high biomass compared to S. cerevisiae. Therefore, the aim of this study was to screen and isolate yeast from rumen fluids with an experimental design method. We optimized a fermentation medium containing sugarcane molasses as a carbon source and urea as a nitrogen source to measure the efficiency of biomass production and cellulase activity. Two fistulated-crossbred Holstein Friesian steers, averaging 350 ± 20 kg body weight, were used to screen and isolate ruminal yeast. The two experiments were designed. A 12 × 3 × 3 factorial was used in a completely randomized design to determine biomass and carboxymethyl cellulase activity. Factor A was isolated yeasts and S. cerevisiae. Factor B was sugarcane molasses (M) concentration. Factor C was urea (U) concentration. Potential yeast was selected for identified and analyzed as a 4 × 3 factorial use in a completely randomized design including. Factor A was incubation times. Factor B was isolated yeast strains including code H-KKU20 (as P. kudriavzevii-KKU20), I-KKU20 (C. tropicalis-KKU20), and C-KKU20 (as Galactomyces sp.-KKU20). Isolation was under aerobic conditions, resulting in a total of 11 different colonies. We noted two appearances of colonies including, asymmetric colonies of isolated yeast (indicated as A, B, C, E, and J) and ovoid colonies (coded as D, F, G, H, I, and K). The highest biomass was observed in three yeasts including codes H, I, and C-KKU20 when inoculated in 25% molasses with 1% urea (M25+U1) (p <0.01). The highest CMCase activity was observed in yeast code H-KKU20 when inoculated in all media solutions (p <0.01). Ruminal yeasts strains H-KKU20, I-KKU20, and C-KKU20 were selected for their ability to produce biomass and their CMCase enzyme synthesis. Identification of isolates H-KKU20 and I-KKU20 revealed that those isolates belonged to Pichia kudriavzevii-KKU20 and Candida tropicalis-KKU20, while C-KKU20 was identified as Galactomyces sp.-KKU20. Two strains provided maximum cell growth: P. kudriavzevii-KKU20 (9.78 and 10.02 Log cell/ml) and C. tropicalis-KKU20 (9.53 and 9.6 Log cells/ml) at 60 and 72 h of incubation time, respectively. The highest ethanol production was observed in S. cerevisiae: 76.4, 77.8, 78.5, and 78.6 g/L at 36, 48, 60, and 72 h of incubation time, respectively (p <0.01). The P. kudriavzevii-KKU20 yielded the least reducing sugar about 30.6 and 29.8 g/L at 60 and 72 h of incubation time, respectively. It could be concluded that screening and isolating yeast from rumen fluids resulted in 11 different characteristics of yeasts. The first novel yeasts discovered in the rumen fluid of cattle were Pichia kudriavzevii-KKU20, Candida tropicalis-KKU20, and Galactomyces sp.- KKU20. P. kudriavzevii-KKU20 had higher results than the other yeasts in terms of biomass production, cellulase enzyme activity, and cell number.

show abstract

“…Moreover, Paserakung et al 5 revealed that Tricosporon asahii GSY10, a yeast isolated from the environment, was a potential alternative fat source in ruminant diets. A recent study by Intanoo, et al 6 reported that isolated Kluyveromyces marxianus yeast from rumen fluid showed potential as an aflatoxin-detoxifying agent in dairy cattle.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Novel Yeasts From Dairy Cattle Rumen for Potential Use as Feed Additives 

Suntara

Cherdthong

Wanapat

et al. 2020

Preprint

View full text Add to dashboard Cite

The aims of this study were to screen and isolate yeast which is producing high biomass and cellulase enzyme from rumen fluid. Two fistulated Thai crossbred Holstein Friesian steers, averaging 350 ± 20 kg body weight, were used. The experiments were designed by 12 × 3 × 3 factorial arrangement in a completely randomized (CRD). Factor A was isolated yeasts (11 difference characteristics) and S. cerevisiae. Factor B was sugarcane molasses (M) concentration (50, 150, and 250 g/L). Factor C was urea (U) concentration (10, 30, and 50 g/L) (experiment 1). To assess biochemical properties, the potential yeast were selected for identified and analyzed. The highest yeast biomass was observed in three yeasts including codes H-KKU20, I-KKU20, and C-KKU20. The highest CMCase activity was observed in yeast code H-KKU20. Identification of isolates H-KKU20 and I-KKU20 revealed that those isolates belonged to Pichia kudriavzevii-KKU20 and Candida tropicalis-KKU20, while C-KKU20 was identified as Galactomyces sp.-KKU20. The P. kudriavzevii-KKU20 and C. tropicalis-KKU20 provided maximum cell growth. The highest ethanol production was observed in S. cerevisiae. The P. kudriavzevii-KKU20 yielded the least reducing sugar. P. kudriavzevii-KKU20 had higher results than the other yeasts in terms of yeast biomass production, cellulase enzyme activity, and cell number. This species may lead to higher production of yeast cell wall than the traditional species in yeast industry (such as S. cerevisiae) or in other applications as ruminant feed additives.

show abstract

Selection and identification of oleaginous yeast isolated from soil, animal feed and ruminal fluid for use as feed supplement in dairy cattle

Abstract: This study illustrated the ability of T. asahii GSY10 to utilize molasses and (NH4 )2 SO4 for synthesizing and accumulating cellular lipid of which oleic acid (C18:1 ) was predominant. This yeast would be used for microbial lipid production used as feed supplement in dairy cattle.

Cited by 17 publications

References 27 publications

Comprehending the influence of critical cultivation parameters on the oleaginous behaviour of potent rotten fruit yeast isolates

Comprehending the influence of critical cultivation parameters on the oleaginous behaviour of potent rotten fruit yeast isolates

Isolation and Characterization of Novel Yeasts from Rumen Fluids for Potential Use as Additives for Ruminant Feeding

Isolation and Characterization of Novel Yeasts From Dairy Cattle Rumen for Potential Use as Feed Additives

Contact Info

Product

Resources

About

Selection and identification of oleaginous yeast isolated from soil, animal feed and ruminal fluid for use as feed supplement in dairy cattle

Abstract: This study illustrated the ability of T. asahii GSY10 to utilize molasses and (NH4 )2 SO4 for synthesizing and accumulating cellular lipid of which oleic acid (C18:1 ) was predominant. This yeast would be used for microbial lipid production used as feed supplement in dairy cattle.

Cited by 17 publications

References 27 publications

Comprehending the influence of critical cultivation parameters on the oleaginous behaviour of potent rotten fruit yeast isolates

Comprehending the influence of critical cultivation parameters on the oleaginous behaviour of potent rotten fruit yeast isolates

Isolation and Characterization of Novel Yeasts from Rumen Fluids for Potential Use as Additives for Ruminant Feeding

Isolation and Characterization of Novel Yeasts From Dairy Cattle Rumen for Potential Use as Feed Additives&nbsp;

Contact Info

Product

Resources

About

Isolation and Characterization of Novel Yeasts From Dairy Cattle Rumen for Potential Use as Feed Additives