Selection and micropropagation of high artemisinin producing clones of <i>Artemisia annua</i> L.

Elhag, Hamid; El-Domiaty, Maher; El‐Feraly, Farouk S.; Mossa, Jaber S.; El-Olemy, M. M.

doi:10.1002/ptr.2650060106

Cited by 35 publications

(22 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Under these conditions the build up of localized humidity could induce the infection (Laughlin and Munro, 1983). In Saudi Arabia, Orobanche cernua was identified as a root parasite of A. annua with the potential to cause losses in yield (Elhag et al, 1997).…”

Section: Insects and Pathogensmentioning

confidence: 99%

Cultivation and genetics ofArtemisia annuaL. for increased production of the antimalarial artemisinin

Ferreira¹,

Laughlin²,

Delabays

et al. 2005

Plant Genet. Resour.

111

View full text Add to dashboard Cite

Malaria has been treated for over 350 years with quinine and quinine-derived drugs. However, in several areas of the world, some strains of the malarial parasite Plasmodium falciparum have developed resistance against these drugs. Recently, the World Health Organization (WHO) recommended the use of artemisinin-combination treatments (ACT) as the first-line treatment for multidrug-resistant falciparum malaria. The WHO estimates that current supplies of artemisinin are sufficient for only 30 million ACT, and is foreseeing the need for 130-220 million ACT in 2005 (WHO, 2004. Current research on the production of synthetic artemisinin-like compounds by the Roll Back Malaria project, pharmaceutical companies and academia resulted in a promising synthetic artemisinin-like compound (OZ277) which is currently undergoing phase I clinical trials. In about 5 years this drug is expected to be approved and made available to the public, however, meeting current global demands for ACT depends on the immediate availability of affordable artemisinin-derived drugs. This will involve expansion of the area under cultivation of Artemisia annua and improved methods of cultivation and processing of raw material, associated with more efficient methods for extraction and purification of artemisinin from plant material. This review addresses the agricultural, environmental and genetic aspects that may be useful in the successful large-scale cultivation of A. annua and for producing the antimalarial artemisinin in areas where it is urgently needed today. It also includes geographic aspects (latitude and altitude), which will help make decisions about crop establishment in tropical countries, and includes a list of Good Agricultural and Collection Practices for A. annua.

show abstract

Section: Insects and Pathogensmentioning

confidence: 99%

Cultivation and genetics ofArtemisia annuaL. for increased production of the antimalarial artemisinin

Ferreira¹,

Laughlin²,

Delabays

et al. 2005

Plant Genet. Resour.

111

View full text Add to dashboard Cite

show abstract

“…Therefore, the plant remains the only valid source of artemisinin. Next to selection procedures of A. annua plants (Singh et al 1988;EISohly et al 1990;Simon et al 1990;Elhag et al 1992), a biotechnological approach has been considered to be an alternative for the production of artemisinin. However, results from experiments with undifferentiated callus and cell suspension cultures of A.…”

Section: Introductionmentioning

confidence: 99%

Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L.

Woerdenbag

Lüers

Udem

et al. 1993

Plant Cell Tiss Organ Cult

108

View full text Add to dashboard Cite

From aseptically grown Artembia annua plantlets, shoot cultures were initiated. Using different concentrations of auxine, cytokinine and sucrose, a suitable culture medium was developed, with respect to the growth of the shoots and their artemisinin accumulation. Nitrate concentration and conductivity appeared to be suitable growth parameters. The artemisinin content was measured gas chromatographically. The shoot cultures were maintained in the developed standard medium, consisting of a half concentration of MS-salts with vitamins, 0.2 mg 1 1 BAP, 0.05 mg 1-1 NAA and 1% sucrose. The growth of the shoots and the artemisinin content remained stable for a longer period. They showed considerable photosynthetic activity and generally contained ca. 0.08% artemisinin on a dry weight basis. The highest artemisinin content found was 0.16% in the above mentioned standard medium, but also on the same medium with 0.5% sucrose. Attempts were made to further improve the artemisinin production by varying the medium composition through addition of gibberellic acid or casein hydrolysate; by omitting plant growth regulators; by precursor feeding, i.e. mevalonic acid; by influencing the biosynthesis routing through inhibition of the sterol synthesis by miconazole, naftifine or terbinafine; by changing gene expression with 5-azacytidine or colchicine; and by elicitation, using cellulase, chitosan, glutathione or nigeran. Enhanced artemisinin production was found with 10 mg 1-1 gibberellic acid, 0.5 g 1 -~ casein hydrolysate, 10 mg 1-1 or 20 mg 1-1 naftifine. Relative increases of 154%, 169%, 140% and 120% were found, respectively. Other additions caused the growth to cease and the artemisinin contents to drop.

show abstract

“…However, Khanam et al (1999) reported that successful rooting was obtained from shoot bunches of jojoba on MS supplemented with 0.2 -2.0 mg/l BA, 2.0 mg IBA mg/l and 5000 mg/l activated charcoal. In this context, attempts were made to root micropropagated shoots of jojoba on various media (half strength of MS or MS with or without IAA, NAA and IBA) (Elhag et al 1998). The highest rate of rooting occurred on the MS containing 3 mg/l IBA.…”

Section: No Nodes/ Primary Shootsmentioning

confidence: 99%

“…In vitro rooting percentage ranged from 20 and 95% depending on the clone and the specific trial. The highest rate of rooting occurred on the medium containing IBA (Elhag et al 1998). Acclimation of in vitro produced micropropagated jojoba plantlets for transfer to field has been difficult, since the necessary high humidity environment can result in lack of aeration and death of the root system (Chaturvedi and Sharma 1989), or because the leaves produced in the culture are of reduced size (Lee 1988).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Bekheet

Gabr

Reda

et al. 2016

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

This study is aimed at developing an efficient method for micropropagation of true-to-type jojoba plants. Nodal segments were used for in vitro shoots proliferation. Among three concentrations of BA used for multiplication, 1 mg/l BA gave the highest number of shoots. To enhance growth of shoots, combinations of BA and Kn were investigated. The greater value of shoot length and the maximum number of nodes were observed in the medium containing 1 mg/l BA + 1.5 mg/l Kn. Among different medium used to increase the rate of multiplication, the maximum number of shoots was recorded at ½ MS + Gamborg B5 (B5) vitamins + 2 mg/l Kn + 10 mg/l adenine sulphate (AS). Rooting was obtained upon supplementation of ½ Woody Plant Medium (WPM) with 1 mg/l IBA. Acclimation was achieved by transplanting rooted plantlets into pots containing peat moss and vermiculite. Start codon targeted (SCoT) and intersimple sequence repeat (ISSR) analyses were carried out to assess the genetic fidelity of micropropagated plantlets. All banding profiles of the two analyses from micropropagated plants were monomorphic and similar to the mother plant. Hence, this protocol can be employed for commercial micropropagation of jojoba without the risk of losing genetic stability.

show abstract

Selection and micropropagation of high artemisinin producing clones of Artemisia annua L.

Cited by 35 publications

References 14 publications

Cultivation and genetics ofArtemisia annuaL. for increased production of the antimalarial artemisinin

Cultivation and genetics ofArtemisia annuaL. for increased production of the antimalarial artemisinin

Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L.

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Contact Info

Product

Resources

About