Selection and molecular identification of fungal isolates that produce xylanolytic enzymes

Alvarez-Navarrete, Mariana; López, G E Reyna; Flores-García, Alberto; Gomez, Ruben; Martínez-Pacheco, Mauro M.

doi:10.4238/2015.july.17.19

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…Nine fungal strains of 103 strains screened for xylanase activity were identified based on sequencing ITS ribosomal gene region using ITS and ITS4 as primers. These identified fungi were species of Fusarium genus as compared to previous identification [35]. In addition, 16 yeast strains from 390 that showing cellulase as primary screened on solid medium.…”

Section: Isolation and Identification Of Fungal Strainsmentioning

confidence: 88%

Evaluation of Antioxidants, Total Phenolics And Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

Shawky¹,

Nagah²,

Ghareeb³

et al. 2019

Journal of Renewable Materials

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Rice straw (RS) collected from Egyptian agricultural environment (Al-Masied village-Alsharqia Governorate-Egypt) was used as substrate for three fungal isolates namely: 4b, 1Asp, Pleurotus ostreatus NRRL 3501 and mixed culture of (4b, 1Asp). Isolates number 4b and 1Asp were identified using the molecular technique (18S rRNA). Isolate 4b and 1Asp, were identified as Trichoderma saturnisporum MN1-EGY and Aspergillus niger MN2-EGY, respectively. Trichoderma saturnisporum MN1-EGY extract exhibited the highest antimicrobial activity compared to the other fungal extracts. Total phenolic, antimicrobial and antioxidant activities were also determined from the ethyl acetate extract of rice straw inoculated by these fungi. The antioxidant scavenging concentration(SC50) values of the tested samples were 88.84, 91.45, 94.65 and 100.0µg/ml, respectively for Pleurotus sp., Aspergiluus niger, Aspergiluus niger + Trichoderma sp., and Trichoderma sp., after 15 days incubation, compared to ascorbic acid as positive control with SC50 value equal to 8.0 µg/ml. Total phenolic contents (TPCs) were maximum in Pleurotus sp. extract and found to be the most polyphenolic enriched sample (380.64 mg GAE/g dry extract) compared to extracts of Aspergiluusniger, Aspergiluus niger + Trichoderma sp., and Trichoderma sp. (261.89, 198.52, and 119.80 mg GAE/g dry extract), respectively. The GC-MS analysis has been also performed for the promising extracts.

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Section: Isolation and Identification Of Fungal Strainsmentioning

confidence: 88%

Evaluation of Antioxidants, Total Phenolics And Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

Shawky¹,

Nagah²,

Ghareeb³

et al. 2019

Journal of Renewable Materials

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“…In recent years, the phytopathogenic fungi have been used as a study model for the production of xylanase with possible industrial applications 30 37 . Also, already they have been purified and characterized xylanases from the phytopathogenic S. sclerotiorum, F. oxysporum, M. grisea, M. oryzae, C. carbonum, S. maydis, S. reilianum, produced in different culture media using tissue of their hosts, identifying different isoenzymes, which may present a role during the life cycle of these fungi 17 18 20 21 22 27 .…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of β-xylanase SRXL1 of Sporisorium reilianum and its relationship with families (GH10 and GH11) of Ascomycetes and Basidiomycetes

Álvarez-Cervantes

Díaz‐Godínez

Mercado-Flores

et al. 2016

Sci Rep

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In this paper, the amino acid sequence of the β-xylanase SRXL1 of Sporisorium reilianum, which is a pathogenic fungus of maize was used as a model protein to find its phylogenetic relationship with other xylanases of Ascomycetes and Basidiomycetes and the information obtained allowed to establish a hypothesis of monophyly and of biological role. 84 amino acid sequences of β-xylanase obtained from the GenBank database was used. Groupings analysis of higher-level in the Pfam database allowed to determine that the proteins under study were classified into the GH10 and GH11 families, based on the regions of highly conserved amino acids, 233–318 and 180–193 respectively, where glutamate residues are responsible for the catalysis.

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“…Fusarium solani (accession number KP137441, GenBank (NCBI)) was isolated from horticultural field crops (22 • 35 ′ 00 ′′ N, 102 • 15 ′ 00 ′′ W) [17]. The Aspergillus sp.…”

Section: Biological Materialsmentioning

confidence: 99%

“…There have been few reports on the efficient production of xylanases and exploratory studies about its xylanolytic activity [14] and optimization of enzyme production [15,16]. In a previous study, Fusarium solani was isolated from bean crops, identified, and selected from a group of one hundred and three fungal isolates for its notable ability to produce extracellular xylanases [17]. Based on the third strategy to reduce the cost of enzymes, corn stover is a potentially profitable source for the fermentative production of fungal xylanases.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Extracellular Holocellulolytic Activity of Fusarium solani and Aspergillus sp. in Corn Stover

Alvarez-Navarrete,

Alonso-Hurtado,

Flores-García

et al. 2024

Fermentation

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Fungal holocellulases are interesting for their possible applications in the bioconversion of corn crop residues into molecules with technological significance. Holocellulase (xylanases and cellulases) production from Fusarium solani and Aspergillus sp. with corn stover as a carbon source was compared using a Box–Wilson design. The fungal holocellulase production was different in both fungi. For F. solani, the maximum endoxylanase and β-xylosidase activities were 14.15 U/mg and 0.75 U/mg at 84 h of fermentation on 350 g/L corn stover, while Aspergillus sp. was 5.90 U/mg and 0.03 U/mg, respectively, at 156 h and 1000 g/L corn stover. The production of holocellulases in both fungi was reduced with increasing carbon sources. The nitrogen source induced the holocellulases in Aspergillus sp., but not in F. solani. Interestingly, when verifying the optimal culture conditions, the production of endoxylanases by F. solani was higher when compared to the predicted value. With regard to the endoxylanase and β-xylosidase activities of Aspergillus sp., these were close to the predicted values. Based on the optimization model, F. solani and Aspergillus sp. produce an interesting holocellulolytic activity in a growth medium with corn stover as the only carbon source. The fermentation time and the amount of corn stover required to obtain maximum holocellulase production are possible advantages for Fusarium solani and Aspergillus sp., respectively.

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Selection and molecular identification of fungal isolates that produce xylanolytic enzymes

Cited by 8 publications

References 34 publications

Evaluation of Antioxidants, Total Phenolics And Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

Evaluation of Antioxidants, Total Phenolics And Antimicrobial Activities of Ethyl Acetate Extracts From Fungi Grown on Rice Straw

Phylogenetic analysis of β-xylanase SRXL1 of Sporisorium reilianum and its relationship with families (GH10 and GH11) of Ascomycetes and Basidiomycetes

Comparative Study of the Extracellular Holocellulolytic Activity of Fusarium solani and Aspergillus sp. in Corn Stover

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