Selection assisted by a <i>BoLA-DR/DQ</i> haplotype against
susceptibility to bovine dermatophilosis

Maillard, Jean-Charles; Berthier, David; Chantal, Isabelle; Thévenon, Sophie; Sidibé, Issa; Stachurski, Frédéric; Belemsaga, Désiré; Razafindraïbé, Hanta; Elsen, Jean-Michel

doi:10.1051/gse:2003027

Cited by 15 publications

(12 citation statements)

References 5 publications

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“…Mentioned similar percentages associated with an outbreak in Argentina where animals with severe signs were systemically affected and had consequently secondary complications that led to the death of some of them. Maillard et al [19] mention that bovine dermatophilosis in tropical areas, may cause severe productive losses, and reach a mortality of 15%.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of Dermatophilus congolensis in Grazing Beef Cattle in Northeastern Mexico: First Report

Covarrubias¹

2015

J Veterinar Sci Technol

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Dermatophilus congolensis is a bacterium that causes exudative dermatitis with scab formation in bovines. Humidity and ticks are predisposing factors. This study describes skin lesions in 27 bovines from a Simbrah herd of grazing livestock of 180 females (15%), aged 18 to 30 months old, in Aldama, Tamaulipas, Mexico. Lesions were distributed in the lower extremities, the belly and the neck and consisted of thick grayish to dark brown scabs with exudates and blood. Scabs were collected from four of the most severely affected animals, placed in sterile tubes and then transported in refrigeration to the laboratory. Samples were processed for isolate D. congolensis. The four samples revealed long branched filaments made up of coccoid cells arranged in parallel rows, one after the other, which is a characteristic of D. congolensis. In this outbreak, morbidity was 15% (27/180) and mortality was 22% (6/27). The PCR using ESP1 and ESP2 primers amplified a product between 400 and 500 bp, and the 16 s sequence was 100% identical to D. congolensis gene for 16S rRNA, strain: NBRC 105199 Sequence ID: dbj|AB550800.1|. The observation of lesions, clinical signs, identification and sequence led to this first report of bovine dermatophilosis in Mexico.

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Section: Discussionmentioning

confidence: 99%

Outbreak of Dermatophilus congolensis in Grazing Beef Cattle in Northeastern Mexico: First Report

Covarrubias¹

2015

J Veterinar Sci Technol

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“…Genes play one of the major roles in disease resistance in bovine species [3][4][5][6][7][8][9][10]. Candidate genes such as BoLA-DRB3 [11,12], FEZ [13], TLR4 [14,15], CARD 15 [16,17], cluster of differentiation 14 (CD14) [18][19][20] and many more genes are associated with mastitis resistance/susceptibility in different cattle breeds.…”

Section: Introductionmentioning

confidence: 99%

Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan fries cattle

Selvan¹,

Gupta²,

Chaudhari³

et al. 2014

Vet World

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Aim: The present study was undertaken with the objectives to characterize, identify DNA polymorphism in cluster of differentiation 14 (CD14) gene in Karan Fries (KF) cattle and to analyze association between genetic variants with incidence of clinical mastitis in National Dairy Research Institute (NDRI) herd, Karnal. Materials and Methods:Genomic DNA was extracted using blood of randomly selected hundred KF lactating cattle by phenol-chloroform method. After checking its quality and quantity, polymerase chain reaction (PCR) was carried out using reported primers to amplify 832 base pair region covering nucleotide base position number 1012 to 1843 (part of promoter, 5'UTR, exon 1, intron 1 and part of exon 2) of bovine CD14 gene. The PCR amplified target product was purified, sequenced and further ClustalW analysis was done to align edited sequence with reported Bos taurus sequence (EU148610.1). The restriction fragment length polymorphism (RFLP) analysis was performed for each KF cow using HinfI restriction enzyme (RE). Cows were assigned genotypes obtained by PCR-RFLP analysis and association study was done using Chi-square (χ 2 ) test.Results: After PCR amplification, DNA sequencing of amplicon confirmed the 832 bases covering 1012 to 1843 nucleotide base position of bovine CD14 gene. ClustalW multiple sequence alignment program for DNA revealed six nucleotide changes in KF cows at positions T1117D, T1239G, T1291C, G1359C, G1361A, and G1811A. Cows were also screened using PCR-RFLP with HinfI RE, which revealed three genotypes CC, CD and DD that differed significantly regarding mastitis incidence. Within CC genotype, 72.73% of cows were in a mastitis non-affected group whereas, those in CD and DD genotypes 69.44% and 60.38% respectively were mastitis affected. Conclusion:KF cows with allele C of CD14 gene were less susceptibility to mastitis compared with D allele.

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“…3,4,6,8 Host age, genetic factors, and immunosuppression related to tick infestation have been associated with increased susceptibility to dermatophilosis in ruminants. 1,5,7 The filly in the current report had no indications of immunocompromise, and she was not an Arabian, which is the horse breed associated with severe combined immunodeficiency. However, no specific testing was performed to evaluate immune function.…”

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confidence: 99%

Atypical Dermatophilus Congolensis Infection in a Three-Year-Old Pony

et al. 2010

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Abstract. Dermatophilosis caused by Dermatophilus congolensis causes exudative dermatitis in a variety of species. The infection is generally limited to the cutaneous tissues, where infection is acquired from the environment and bacteria penetrate into keratinized epithelium through epithelial disruption. A 3-year-old pony filly was examined for enlarging mandibular lymph nodes during the preceding 10 months. Biopsy of the node revealed mixed and granulomatous inflammation and thick, filamentous Gram-positive bacteria. Dermatophilus congolensis, confirmed by biochemical testing and sequencing of the ribosomal RNA gene, was cultured from a sample aspirated from the infected site. Dermatophilus congolensis should be considered as a possible etiologic agent associated with lymphadenopathy and granulomatous inflammation in the horse.Key words: Dermatophilosis; Dermatophilus congolensis; horses; lymphadenopathy.Dermatophilus congolensis is a Gram-positive coccobacillary actinomycete that causes an exudative dermatitis in a variety of species, most notably in ruminants and horses, although rare infections occur in cats, dogs, and humans.1,2,12 Infection is generally restricted to the skin and subcutaneous tissue, although uncommon involvement of lymphoid tissue in ruminants and cats has been described. 3,4,12A 3-year-old female palomino pony was referred to the William R. Pritchard Veterinary Teaching Hospital at the University of California, Davis (Davis, CA) with the primary complaint of progressively enlarging bilateral mandibular lymphadenopathy during the previous 10 months. Intermandibular swelling was noticed the winter of the previous year (10 months before referral), and a biopsy of the affected area revealed inflamed lymphoid tissue. Prior to referral, the first premolar teeth had erupted and were removed; significant hooks on cheek teeth were treated with dental floatation. The pony was also reported by the owner to be lethargic and appeared pruritic, as evidenced by biting at or rubbing her sides. The filly had been regularly vaccinated and had most recently been dewormed with pyrantel pamoate 3 weeks prior to referral.The filly was slightly depressed on examination. Her temperature and heart rate were within normal limits. A slightly elevated respiratory rate of 25 breaths per minute was detected, although there were no abnormal lung sounds on thoracic auscultation. Large, firm, nonpainful multilobulated masses in the intermandibular space were palpable. The only other abnormalities on physical examination were small areas of superficial cutaneous excoriations over the flanks, thorax, and gluteal musculature. Dark, loose fecal material was observed after normal defecation.A complete blood count was unremarkable, and plasma fibrinogen was 300 mg/dl. A serum biochemistry panel was within normal limits, with the exceptions of slightly elevated alkaline phosphatase ( A lymph node aspirate and biopsy were performed on the affected tissue; a small amount of inspissated pus was observed during the biopsy procedu...

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Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

Cited by 15 publications

References 5 publications

Outbreak of Dermatophilus congolensis in Grazing Beef Cattle in Northeastern Mexico: First Report

Outbreak of Dermatophilus congolensis in Grazing Beef Cattle in Northeastern Mexico: First Report

Cluster of differentiation 14 gene polymorphism and its association with incidence of clinical mastitis in Karan fries cattle

Atypical Dermatophilus Congolensis Infection in a Three-Year-Old Pony

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