Selection Criteria of Cord Blood Units for Platelet Gel Production: Proposed Directions from Hellenic Cord Blood Bank. Comment on Mallis et al. Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study. Bioengineering 2019, 6, 66

Mallis, Panagiotis; Michalopoulos, Efstathios; Panagouli, Effrosyni; Dimou, Zetta; Sarri, Eirini Faidra; Georgiou, Eleni; Gkioka, Vasiliki; Stavropoulos-Giokas, Catherine

doi:10.3390/bioengineering8050053

Cited by 4 publications

(2 citation statements)

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“…Good results have also been accomplished for spinal reflex recovery in animal models, wound healing, and reconstruction of deep surgical sites after cardiothoracic surgery. Considering the huge amount of cord blood discarded from most cord blood banks (more than 80%) due to a low stem cell count, and the similar quantity of platelets with no distinction regarding their function contained in both cord blood units and adult peripheral blood samples, cord blood platelet gel production should be considered when possible [97][98][99]. Moreover, superior therapeutic effects for cord blood PRP over adult peripheral blood PRP have been suggested due to a more anti-inflammatory molecule profile for cord blood PRP than adult peripheral blood PRP [100].…”

Section: Discussion and Future Directionsmentioning

confidence: 99%

Platelet-Rich Plasma (PRP) in Dermatology: Cellular and Molecular Mechanisms of Action

Vladulescu,

Scurtu,

Simionescu

et al. 2023

Biomedicines

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Platelet-rich plasma (PRP) therapy has gained attention in the scientific field due to its potential regenerative effects and great benefit–risk ratio. This review extensively explores the most studied mechanisms of this therapy according to the etiopathogenesis of skin diseases: cellular proliferation, matrix formation, regulation of inflammation, angiogenesis, collagen synthesis, and the remodeling of new tissue. Moreover, it draws on newly reported and lesser-known effects of PRP: its anti-apoptotic effects, immunological suppression, decrease in melanin synthesis, anti-microbial effects, overexpression of miR-155, antioxidant effects, and their involved pathways. This work aims to provide a complete update for understanding PRP’s benefits and clinical relevance in wound healing, alopecia, pigmentary disorders, scars, rejuvenation, lichen sclerosus, and other inflammatory dermatoses, based on the current evidence. Furthermore, recent reports with novel indications for PRP therapy are highlighted, and new potential pathways correlated with the pathogenesis of skin diseases are explored.

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Section: Discussion and Future Directionsmentioning

confidence: 99%

Platelet-Rich Plasma (PRP) in Dermatology: Cellular and Molecular Mechanisms of Action

Vladulescu,

Scurtu,

Simionescu

et al. 2023

Biomedicines

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show abstract

“…The concentration and the combination of the above proteins have been shown to present tissue remodeling properties and also favor cell adhesion, proliferation and differentiation [ 34 ]. Currently, CBPL has been used widely in personalized regenerative medicine applications including wound and burn healing, and the regeneration of long-term skin ulcers in diabetic patients, while the standardization criteria for its proper production have also been described and published [ 35 , 36 , 37 ]. Therefore, the utilization of CBPL may improve the repopulation efficacy of the decellularized hUAs.…”

Section: Introductionmentioning

confidence: 99%

Improved Repopulation Efficacy of Decellularized Small Diameter Vascular Grafts Utilizing the Cord Blood Platelet Lysate

et al. 2021

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Background: The development of functional bioengineered small-diameter vascular grafts (SDVGs), represents a major challenge of tissue engineering. This study aimed to evaluate the repopulation efficacy of biological vessels, utilizing the cord blood platelet lysate (CBPL). Methods: Human umbilical arteries (hUAs, n = 10) were submitted to decellularization. Then, an evaluation of decellularized hUAs, involving histological, biochemical and biomechanical analysis, was performed. Wharton’s Jelly (WJ) Mesenchymal Stromal Cells (MSCs) were isolated and characterized for their properties. Then, WJ-MSCs (1.5 × 106 cells) were seeded on decellularized hUAs (n = 5) and cultivated with (Group A) or without the presence of the CBPL, (Group B) for 30 days. Histological analysis involving immunohistochemistry (against Ki67, for determination of cell proliferation) and indirect immunofluorescence (against activated MAP kinase, additional marker for cell growth and proliferation) was performed. Results: The decellularized hUAs retained their initial vessel’s properties, in terms of key-specific proteins, the biochemical and biomechanical characteristics were preserved. The evaluation of the repopulation process indicated a more uniform distribution of WJ-MSCs in group A compared to group B. The repopulated vascular grafts of group B were characterized by greater Ki67 and MAP kinase expression compared to group A. Conclusion: The results of this study indicated that the CBPL may improve the repopulation efficacy, thus bringing the biological SDVGs one step closer to clinical application.

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Investigating the production of platelet lysate obtained from low volume Cord Blood Units: Focus on growth factor content and regenerative potential

Mallis¹,

Michalopoulos²,

Balampanis³

et al. 2022

Transfusion and Apheresis Science

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Selection Criteria of Cord Blood Units for Platelet Gel Production: Proposed Directions from Hellenic Cord Blood Bank. Comment on Mallis et al. Short Term Results of Fibrin Gel Obtained from Cord Blood Units: A Preliminary in Vitro Study. Bioengineering 2019, 6, 66

Cited by 4 publications

References 13 publications

Platelet-Rich Plasma (PRP) in Dermatology: Cellular and Molecular Mechanisms of Action

Platelet-Rich Plasma (PRP) in Dermatology: Cellular and Molecular Mechanisms of Action

Improved Repopulation Efficacy of Decellularized Small Diameter Vascular Grafts Utilizing the Cord Blood Platelet Lysate

Investigating the production of platelet lysate obtained from low volume Cord Blood Units: Focus on growth factor content and regenerative potential

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