Selection for high and low oxygen consumption‐induced differences in maintenance energy requirements of mice

Abstract

Maintenance energy requirements (MER) of mice selected for high (H) or low (L) oxygen consumption (OC) were compared. Forty-four mice from H and L OC lines were weaned at 3 weeks and divided into four experimental groups: group A were sacrificed at 4 weeks; group B were fed ad libitum, and groups C and D were fed 2.8 and 2.4 g/day, respectively, from 4 to 8 weeks of age. Groups B-D were sacrificed at 8 weeks. Chemical components were estimated for all groups. MER was estimated using a model that partitioned metabolizable energy intake into that used for maintenance, and protein and fat deposition. The feed conversion ratio for the B group was significantly higher in the H than in the L line. Feed intake for metabolic energy content per metabolic body size was significantly also higher in the H line, whereas accumulated energy content per metabolic body size was significantly higher in the L line. MER of the H line was greater than that of the L line (P < 0.10). These results suggest that selection for H or L OC produced differences in chemical components, feed efficiency, and MER between the H and L lines.

Correlations between mitochondrial respiration activity and residual feed intake after divergent genetic selection for high‐ and low‐ oxygen consumption in mice

“…Significant differences in FCR (Hong et al, ,) and MER (Darhan et al, ) between the H and L mouse lines were previously reported. In the present study, RFI was calculated from FI measurements in 37 mice of the 16th generation.…”

“…It has been reported that the proton leak is associated with MER (rat: Rolfe & Brand, 1996;Rolfe & Brown, 1997;poultry: Luiting, Schrama, Hel, & Urff, 1991). We reported previously that a high-OC mouse line had significantly higher MER than a low-OC mouse line (Darhan et al, 2017). As the liver mitochondrial proton leak was higher in the H than in the L line, the basal metabolic rate clearly differed between them.…”

“…Therefore, we selected for both high and low OC and constructed genetically different OC in mice (Hong, Komaki, Shimazu, & Suzuki, 2013). The high and low-OC mice differed in terms of FCR, MER, hepatic mitochondrial respiration activity, and proton leak (Darhan et al, 2017;Hong et al, 2015Hong et al, , 2013. However, the correlations between mitochondrial respiration activity and feed efficiency were not estimated because both parameters must be measured in the same individual.…”

Genetic and genomic analysis of oxygen consumption in mice

“…Genetics Selection Evolution, 24, 487-509. https://doi.org/10.1186/1297-9686-24-6-487 Meyer, K., & Tier, B. (2012 (Darhan et al, 2017(Darhan et al, , 2019Hong et al, 2013Hong et al, , 2015. OCMBW: OC per metabolic body weight; AgH and AgL: high and low aggressive lines established by divergent selection by Kawamoto et al (1993).…”

Candidate Gene Identification of Feed Efficiency and Coat Color Traits in a C57BL/6J × Kunming F2 Mice Population Using Genome-Wide Association Study

“…Recent study showed that oxygen consumption was negatively associated with FE of mice. Also, the higher oxygen consumption mice have higher mitochondrial activity and energy expenditure than lower oxygen consumption mice [ 9 , 10 ]. These results indicated that energy metabolism was negatively related to FE in mice.…”