Selection, identification and application of a DNA aptamer against Staphylococcus aureus enterotoxin A

A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins

“…With the superior optical features of the UCNPs, the high affinity and specificity of SEB aptamers, and the introduction of exonuclease I into the analyte recycling system, excellent sensitivity (LOD of 0.3 pg/mL) was obtained using this protocol. Moreover, the Wang group enlarged the use of aptamer-based bioassay application in SE detection by selecting aptamers targeted to SEA and SEC 1 , based on magnetic separation technology [ 121 , 122 ]. Combining the SEB aptamer selected by DeGrasse, the Wang group established the simultaneous detection of multiplex SEs (SEA, SEB and SEC1) using multicolor lanthanide-doped TRF labeled aptamers as bioprobes and graphene oxide (GO) as a resonance energy acceptor [ 123 ].…”

Efficient and selective DNA modification on bacterial membranes

“…3,24 For example, a number of DNA aptamer molecules have been identified to selectively recognize specific bacterial membrane targets and further used for the pathogen detection. 25–28 However, the membrane modification efficiency and affinity of these aptamers are often limited. In addition, the identification of aptamers for many bacterial strains is still challenging with the current time-consuming and labor-intensive screening process.…”

Aptasensors for Staphylococcus aureus Risk Assessment in Food

“…The concept of aptamer was first proposed by Ellington and Szostak (1990) . So far, many aptamers have been developed for S. aureus and its toxins ( DeGrasse, 2012 ; Baumstummler et al, 2014 ; Huang et al, 2014 , 2015 ).…”